Preparation method and application of diagnostic antigens secreted by Fasciola grandis excretory products
A technology of flukes and flakes, applied in the field of preparation of diagnostic antigens secreted and excreted by flukes, can solve the problems of insufficient effect and achieve the effects of easy judgment, fast detection, and simple operation
Active Publication Date: 2022-08-05
GUANGXI UNIV
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Problems solved by technology
FgESP as a diagnostic antigen is usually limited to laboratory research at present, and its effect in early diagnosis is not significant enough, and its effective components need to be further explored to optimize its diagnostic effect
Method used
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Embodiment Construction
[0043] In order to make the purpose, technical solutions and advantages of the present invention more clear and clear, the following combination of embodiments to further explain the present invention in detail. It should be understood that the specific embodiments described here are only used to explain the invention and do not limit the present invention.
[0044] experiment:
[0045] 1.1 Fasciola Gigantica Excretory-SECRETORYPRODUcts, FGESP)
[0046] 1. Equipment preparation: Put the equipment in advance, put the equipment in advance, put it in the drying box to dry, and put the drying equipment in the ultraviolet sterilization of ultraviolet for 30 minutes before use. temperate box.
[0047] 2. Cultivate worm bodies: Collection from the slaughterhouse around Guangxi University in Nanning, collect large -shaped buffalo gallbladder, collect large -shaped insects in the gallbladder, carefully select the complete and non -damaged insects in the flat dish with a brush, and use PBS ...
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Abstract
The invention provides a preparation method and application of a diagnostic antigen secreted by Fasciola fasciola excretion products, and a method for preparing an active ingredient of a diagnostic antigen secreted by Fasciola fasciola excretions, which is characterized by comprising the following steps: (1) Fasciola maxima secreted and excretory products Preparation; (2) Collection of chromatographic components; (3) Screening of chromatographic components. The present invention also provides a kit for detecting / assisting Fascioliasis, the kit contains the F22 chromatography component of claim 1. The invention also relates to the application of containing the diagnostic antigen in detecting / assisting the early diagnosis of fascioliasis in buffalo. The F22 chromatographic component screened out by the invention has the advantages of rapid detection, simple operation, easy determination and the like as a diagnostic antigen of Fasciola maxima secreted and excretory products, and can provide a practical and effective method for the detection of Fasciola maxima.
Description
Technical field [0001] The present invention involves a diagnostic antigen, which specifically involves a large-shaped insect-absorbent secretion of excretion products (FGESP) to diagnose the preparation method and application of the antigen containing the antigen containing the antigen. Background technique [0002] Fasciola SPP. The genus Fasciola Hepatica and large -shaped insects are the most common. [0003] Large -shaped insect -absorbing bamboo leaves, not obvious shoulders, dull at the end. The body length is 25 to 50mm, the body length and body width are 3: 1, and the abdominal suction cup is about 1.5 times the oral suction cup. The testicular branch is complicated, accounting for 1 / 2, and the ovarian branch is large. The structure of the adults of the large -shaped insects is observed by the structure. The body surface has a body spinal, and the worm can be divided into body quilts, muscle layers, and cellular layers. The history of the life -shaped insect -absorbing l...
Claims
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IPC IPC(8): G01N33/569G01N33/535G01N33/68G01N33/543G01N33/96C07K1/16C07K1/14C12N9/64C07K14/78C07K14/435C12N9/08
CPCG01N33/56905G01N33/535G01N33/6854G01N33/543G01N33/96C07K1/16C07K1/145C12N9/641C12Y304/22015C12N9/0065C12Y111/01015C07K14/78C07K14/43559
Inventor 张为宇侯林静吴文德靳纬坤吴正姣
Owner GUANGXI UNIV