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Special device for tumor transplantation of zebrafish larvae, and working method of special device

A technology of tumor transplantation and special device, which is applied in veterinary instruments, medical science, etc., can solve the problems of inability to accurately calculate the actual dose of transplanted tumor, inability to achieve accurate quantification of injection volume, inaccurate dose of exogenous tumor transplantation, etc., and achieve the effect. Good, the effect of ensuring the accuracy of the experiment and saving the experiment time

Pending Publication Date: 2019-12-06
BIOLOGY INST OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the length and thickness of the injection needle seriously affect the injection volume, under the same injection pressure and injection time, if the injection needle is replaced, the injection volume is likely to change, and the corresponding dose of the transplanted tumor will also change.
When the injection pressure and time are constant, the volume of the injection solution in the juvenile fish can only be observed with the naked eye and with the help of an indicator, so that the accurate quantification of the injection volume cannot be achieved, and the actual dose of the transplanted tumor cannot be accurately calculated.
[0005] In view of the defects of the existing culture dish for tumor transplantation, it is urgent to design a special device for zebrafish juvenile tumor transplantation to solve the problem of inaccurate exogenous tumor transplantation dose in the existing transplantation process

Method used

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  • Special device for tumor transplantation of zebrafish larvae, and working method of special device
  • Special device for tumor transplantation of zebrafish larvae, and working method of special device
  • Special device for tumor transplantation of zebrafish larvae, and working method of special device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] A dedicated device for tumor transplantation in juvenile zebrafish, such as Figure 1-5 As shown, it includes a transparent box body 1 and a graft body 2, and the box body 1 and the graft body 2 are combined into a whole. The material of the box body 1 is transparent glass or plastic sheet; the shape of the box body 1 is rectangular. The four sides of the rectangular box body 1 are provided with baffles 101, 102, and the material of the baffles 101, 102 is glass or plastic. Whole box body 1 is surrounded by the baffle plate of four weeks and forms a rectangular concave body. The material of the transplant body 2 is transparent glass or plastic sheet; the shape of the transplant body 2 is rectangular, and the four sides of the rectangular transplant body 2 are provided with baffle plates 201, 202, and the material of the baffle plates 201, 202 is glass or plastic. The whole transplant body 2 is surrounded by the surrounding baffles to form a rectangular concave body. ...

Embodiment 2

[0069] A special device for tumor transplantation in juvenile zebrafish, the structure of which is as described in Example 1, the difference is that it includes a transparent box body and a transplant body, and the box body and the transplant body are combined as a whole. The box body is 70mm long, 50mm wide, and 1.5mm thick, and the surrounding baffles are 10mm high and 1.5mm thick. The transplant body is 50mm long, 45mm wide, and 2mm thick, and the surrounding baffles are 10mm high and 0.5mm thick. The horizontal handles on both sides are 45mm long, 5mm wide and 5mm thick. A circular groove with a length of 45mm, a depth of 4mm, and a wide mouth length of 6mm is arranged on the horizontal handle.

[0070] Four rectangular parallelepiped columns with a length of 2 mm, a width of 1 mm, and a height of 2 mm were set at the four corners of the lower bottom of the implant; four rectangular grooves with a distance of 7 mm, a length of 40 mm, a width of 5 mm, and a depth of 1.5 mm...

Embodiment 3

[0072] A special device for zebrafish juvenile tumor transplantation, its structure is as described in Example 1, the difference is that the box body is 110 mm long, 70 mm wide, and 2.5 mm thick, and the surrounding baffles are 30 mm high and 2.5 mm thick. The transplant body is 100mm long, 60mm wide, and 6mm thick, and the surrounding baffles are 30mm high and 2.5mm thick. The horizontal handles on both sides are 60mm long, 20mm wide and 13mm thick. An arc-shaped groove with a length of 60mm, a depth of 8mm, and a wide mouth length of 15mm is set on the horizontal handle.

[0073] Four rectangular parallelepiped columns with a length of 4 mm, a width of 3 mm, and a height of 5 mm were set at the four corners of the lower bottom of the implant, and six rectangular grooves with a distance of 3 mm, a length of 50 mm, a width of 15 mm, and a depth of 4.7 mm were set on the inner bottom. The inner bottom surface of each rectangular groove is provided with a scale and 60 circular ...

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Abstract

The invention relates to a special device for tumor transplantation of zebrafish larvae, and a working method of the special device. The special device comprises a box body and a transplantation body,the box body and the transplantation body are each of a rectangular concave body with baffles being arranged around, and the transplantation body and the box body are combined into a whole when the transplantation body is inserted into the box body. Four cuboid vertical columns are arranged at the four corners of the lower bottom face of the transplantation body correspondingly, and a plurality of grooves are formed in the inner bottom face of the transplantation body; and a plurality of drainage holes and a scale are arranged on the inner bottom face of each groove, and a plurality of sets of scale marks are arranged on the left inner side wall and the right inner side wall of each groove. According to the special device, the position of the larvae is fixed during tumor transplantation advantageously, and operation is convenient; and the dosage of transplanted tumor cells can also be quantified accurately. In addition, through the special device, the situation that the larvae are sucked mistakenly in the process of sucking excess liquid before tumor transplantation, and consequently, the larvae are damaged can further be effectively avoided; the transplantation time is saved advantageously, and the situation that the operation time is excessively long, consequently, the larvae die due to water shortage is avoided; and the culture device is ingenious in design concept, convenient to use, obvious in effect and good in effect, experiment accuracy is ensured, and the working efficiency is improved.

Description

technical field [0001] The invention relates to a special device and working method for zebrafish juvenile tumor transplantation, and belongs to the technical field of experimental animal research devices. Background technique [0002] Model organism tumor transplantation is an important method in the study of tumor biology. Tumor transplantation is particularly important for the analysis and study of cell malignancy and migration ability, and is the key to identifying potential cancer stem cells. In addition, model biological tumor transplantation also plays an important role in drug research and can be used to screen drugs with tumor-suppressing activity or optimize tumor treatment regimens. [0003] Zebrafish (Danio rerio) is one of the three commonly used model organisms in the field of life sciences. It not only has the characteristics of high egg production, low feeding cost, short reproductive cycle, in vitro fertilization, in vitro development, and transparent embr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D7/00
CPCA61D7/00
Inventor 孙晨刘可春何秋霞韩利文王雪张云王希敏王荣春李晓彬陈锡强郭敬兰
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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