High-performance small and dense low-density lipoprotein cholesterol detection kit

A technology for low-density lipoprotein and detection kits, which can be used in the determination/inspection of microorganisms, biochemical equipment and methods, etc., and can solve problems such as transformation

Pending Publication Date: 2019-12-13
WUHAN HANHAI NEW ENZYMES BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the present invention provides a high-performance small and dense low-density lipoprotein cholesterol detection kit, which solves the problem of the existing small and dense low-density l

Method used

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  • High-performance small and dense low-density lipoprotein cholesterol detection kit

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Embodiment 1

[0023] The raw materials of a high-performance small and dense low-density lipoprotein cholesterol detection kit include by weight: Reagent A: Good's buffer 90mmol / L, cholesterol esterase 1ku / L, cholesterol oxidase 1ku / L, phospholipase 0.7ku / L, catalase 400ku / L and N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline sodium salt (TOOS) 1mmol / L; Reagent B: Good's buffer 90mmol / L, peroxidase 2ku / L, 4-aminoantipyrine 3mmol / L and sodium azide 0.04%, the pH value of Good's buffer is 7.0, the phospholipase is sphingomyelinase, the peroxidase The pH is 6.8.

[0024] In the present invention, the detection method of the high-performance small and dense low-density lipoprotein cholesterol detection kit specifically comprises the following steps:

[0025] S1. Elimination of non-sdLDL-C: take serum or plasma samples, add reagent A to the serum or plasma samples, and remove non-sdLDL-C components first under the action of cholesterol lipase, cholesterol oxidase, phospholipase and catalas...

Embodiment 2

[0028] The raw materials of a high-performance small and dense low-density lipoprotein cholesterol detection kit include: Reagent A: Good's buffer 100mmol / L, cholesterol esterase 2ku / L, cholesterol oxidase 1.5ku / L, phospholipase 0.8 ku / L, catalase 500ku / L and N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline sodium salt (TOOS) 2mmol / L; Reagent B: Good's buffer solution 100mmol / L, peroxidase 2.5ku / L, 4-aminoantipyrine 4mmol / L and sodium azide 0.05%, the pH value of Good's buffer is 7.2, phospholipase is sphingomyelinase, peroxide The pH of the enzyme is 7.0.

[0029] In the present invention, the detection method of the high-performance small and dense low-density lipoprotein cholesterol detection kit specifically comprises the following steps:

[0030]S1. Removal of non-sdLDL-C: Take serum or plasma samples, add reagent A to the serum or plasma samples, and remove non-sdLDL-C components first under the action of cholesterol lipase, cholesterol oxidase, phospholipase and cat...

Embodiment 3

[0033] The raw materials of a high-performance small and dense low-density lipoprotein cholesterol detection kit include by weight: Reagent A: Good's buffer 110mmol / L, cholesterol esterase 3ku / L, cholesterol oxidase 2ku / L, phospholipase 0.9ku / L, catalase 600ku / L and N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline sodium salt (TOOS) 3mmol / L; Reagent B: Good's buffer 110mmol / L, peroxidase 3ku / L, 4-aminoantipyrine 5mmol / L and sodium azide 0.06%, the pH value of Good's buffer is 7.4, the phospholipase is sphingomyelinase, the peroxidase The pH value is 7.2.

[0034] In the present invention, the detection method of the high-performance small and dense low-density lipoprotein cholesterol detection kit specifically comprises the following steps:

[0035] S1. Removal of non-sdLDL-C: Take serum or plasma samples, add reagent A to the serum or plasma samples, and remove non-sdLDL-C components first under the action of cholesterol lipase, cholesterol oxidase, phospholipase and cat...

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Abstract

The invention discloses a high-performance small and dense low-density lipoprotein cholesterol detection kit. The kit comprises the following raw materials in parts by weight; a reagent A: 90 to 110 mmol/L of a Good's buffer solution; 1-3 ku/L of cholesterol esterase, 1-2 ku/L of cholesterol oxidase, 0.7-0.9 ku/L of phospholipase, 400-600 ku/L of catalase, and 1-3 mmol/L N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline sodium salt (TOOS); and a reagent B: 90-110 mmol/L of the Good's buffer solution, 2-3 ku/L of peroxidase, 3-5 mmol/L of 4-aminoantipyrine and 0.04-0.06% of sodium azide. Theinvention relates to the technical field of lipoprotein cholesterol detection, and discloses the high-performance small and dense low-density lipoprotein cholesterol detection kit. The non-sdLDL-C components are removed through cholesterol lipase, cholesterol oxidase, phospholipase and catalase; the interference of other lipoprotein cholesterol on the detection process is avoided, the capacity ofthe small and dense low-density lipoprotein cholesterol in serum can be directly determined by using a full-automatic biochemical analyzer, and the kit is suitable for the requirements of clinical andlaboratory on the determination of the content of the small and dense low-density lipoprotein cholesterol.

Description

technical field [0001] The invention relates to the technical field of lipoprotein cholesterol detection, in particular to a high-performance small and dense low-density lipoprotein cholesterol detection kit. Background technique [0002] Sterols often exist in the form of lipoproteins in the blood, and low-density lipoproteins in plasma are the main carrier for transporting endogenous cholesterol, which are degraded and transformed by binding to low-density lipoprotein receptors on the cell membrane. Low-density lipoprotein cholesterol is composed of LDL-C, which is the main lipoprotein in fasting plasma, accounting for about 2 / 3 of plasma lipoproteins, and is the main vehicle for transporting cholesterol to extrahepatic tissues; LDL-R dysfunction can cause plasma LDL The scavenging ability of -C is reduced, which eventually leads to the formation of atherosclerotic plaque in the arterial intima. Therefore, the content of LDL-C is related to the incidence of cardiovascular...

Claims

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Application Information

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IPC IPC(8): C12Q1/60C12Q1/44C12Q1/30C12Q1/26
CPCC12Q1/60C12Q1/44C12Q1/30C12Q1/26C12Q2326/96
Inventor 杨广宇谢渊王存理
Owner WUHAN HANHAI NEW ENZYMES BIOLOGICAL TECH CO LTD
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