Extraction and purification method of hemerocallis fulva polyphenols

A purification method and technology of Hemerocallis flower, applied in the field of extraction and purification of Hemerocallis flower polyphenols, can solve problems such as the lack of extraction, separation and purification of Hemerocallis flower polyphenols, and achieve the effects of reducing extraction cost, avoiding damage, and improving extraction efficiency

Inactive Publication Date: 2019-12-20
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still a lack of relevant research on the extraction, separation and purification of polyphenols from Hemerocallis flower

Method used

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  • Extraction and purification method of hemerocallis fulva polyphenols
  • Extraction and purification method of hemerocallis fulva polyphenols

Examples

Experimental program
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Effect test

Embodiment 1

[0029] This embodiment provides a method for extracting and purifying polyphenols from Hemerocallis flower, the specific steps are as follows:

[0030] Step 1: Raw material crushing: wash the fresh Hemerocallis flowers and put them into a constant temperature blast dryer to dry for 36 hours at a temperature of 50°C. After drying, crush them and pass through a 100-mesh sieve to obtain Hemerocallis flowers powder, which is set aside;

[0031] Step 2: Ultrasonic extraction: use distilled water as the extraction agent, add Hemerocallis flower powder into distilled water, the mass ratio of solid to liquid is 1:20, the ultrasonic working frequency is 40kHz, the extraction temperature is 30°C, and the extraction time is 50min, and the Hemerocallis is obtained by extraction Flower polyphenols crude extract;

[0032] Step 3: Separation and purification of macroporous resin: put D101 macroporous adsorption resin into a 1.8×40cm chromatography column by wet method, and the filling height...

Embodiment 2

[0036] This embodiment provides a method for extracting and purifying polyphenols from Hemerocallis flower, the specific steps are as follows:

[0037] Step 1: Raw material crushing: wash the fresh Hemerocallis flowers and put them into a constant temperature blast dryer to dry for 48 hours at a temperature of 65°C. After drying, crush them and pass through a 100-mesh sieve to obtain Hemerocallis flowers powder, which is set aside;

[0038] Step 2: Ultrasonic extraction: use distilled water as the extraction agent, add Hemerocallis flower powder into distilled water, the mass ratio of solid to liquid is 1:25, the ultrasonic working frequency is 40kHz, the extraction temperature is 45°C, the extraction time is 65min, and the Hemerocallis is extracted Flower polyphenols crude extract;

[0039] Step 3: Separation and purification of macroporous resin: AB-8 macroporous adsorption resin was wet-packed into a 1.8×40cm chromatography column with a filling height of 30cm to obtain a m...

Embodiment 3

[0043] This embodiment provides a method for extracting and purifying polyphenols from Hemerocallis flower, the specific steps are as follows:

[0044] Step 1: Raw material crushing: wash the fresh Hemerocallis flowers, put them into a constant temperature blast dryer, dry them for 60 hours at a temperature of 80°C, crush them after drying, and pass through a 100-mesh sieve to obtain Hemerocallis flower powder, which is set aside;

[0045] Step 2: Ultrasonic extraction: use distilled water as the extraction agent, add Hemerocallis flower powder into distilled water, the mass ratio of solid to liquid is 1:30, the ultrasonic working frequency is 40kHz, the extraction temperature is 60°C, the extraction time is 80min, and the Hemerocallis is extracted Flower polyphenols crude extract;

[0046]Step 3: Separation and purification of macroporous resin: put HPD600 macroporous adsorption resin macroporous resin into a 1.8×40cm chromatography column by wet method, and the filling heigh...

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Abstract

The invention relates to an extraction and purification method of hemerocallis fulva polyphenols, and aims to provide an efficient extraction, separation and purification method from hemerocallis fulva, which is simple and easy to operate. The extraction and purification method of the polyphenols from the hemerocallis fulva comprises the steps of (1) pretreating the hemerocallis fulva; (2) performing ultrasonic auxiliary extraction for preparation of the hemerocallis fulva polyphenols; (3) performing separation and purification with macroporous resins to obtain an extracting solution; (4) concentrating the extracting solution; and (5) performing freeze drying to obtain hemerocallis fulva polyphenols purification substances.

Description

technical field [0001] The invention belongs to a method for extracting and purifying polyphenols from daylily flowers, in particular to a method for extracting and purifying polyphenols from daylily flowers. Background technique [0002] Hemerocallis fulva (scientific name: Hemerocallis fulva), also known as forget worry grass, is a perennial herb of Liliaceae, belonging to the order Lilium. Daylily flowers generally germinate earlier in spring, and there are many varieties, and the colors of the petals are also colorful. As the "mother flower", Hemerocallis not only has high ornamental value, but also has rich nutritional and medicinal value, rich in a variety of biologically active substances, such as polyphenols, flavonoids, lactams, carotenoids, alkaloids And steroids, etc., is a kind of food and medicine with great development prospects. However, there is still a lack of relevant research on the extraction, separation and purification of polyphenols from Hemerocallis...

Claims

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Application Information

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IPC IPC(8): A61K36/896
CPCA61K36/896A61K2236/15A61K2236/17A61K2236/331A61K2236/51A61K2236/55
Inventor 于海燕石其璇陈臣田怀香
Owner SHANGHAI INST OF TECH
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