Variable frequency laser-based scanning imaging system and method

A technology of scanning imaging and laser scanning device, which is applied in the field of scanning imaging system and scanning imaging system based on frequency conversion laser, can solve the problem that the resolution of laser confocal microscope does not meet the final needs of scientific research workers, and achieve the goal of improving spatial resolution Effect

Active Publication Date: 2019-12-20
杭州科洛码光电科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the confocal laser microscopy system, the improvement of resolution is an important issue. At present, the spatial resolution of laser confocal scanning has been improved by using double-beam dissipation excitation and spatial frequency modulation excitation. However, even with the introduction of various innovative methods, the resolution of laser confocal microscopy still does not meet the ultimate needs of scientific researchers

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  • Variable frequency laser-based scanning imaging system and method

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Embodiment 1

[0033] Such as figure 1 As shown, this embodiment discloses a scanning imaging system based on variable frequency laser, including: a digital signal generator 1, a laser 2, a laser scanning device 3, a microscope module 4, a confocal detection module 5 and a digital acquisition card 6; wherein:

[0034] The laser scanning device 3 includes two sets of scanning modules and a reflecting mirror. The two sets of scanning modules are respectively an X-axis scanning module and a Y-axis scanning module. Each scanning module includes a scanning vibrating mirror 31, a scanning lens 32 and a tube lens 33; For ease of distinction, the components in the X-axis scanning module are named respectively: the first scanning galvanometer 31a, the first scanning lens 32a and the first tube lens 33a, and the components in the Y-axis scanning module are respectively named They are: the second scanning galvanometer 31b, the second scanning lens 32b and the second tube lens 33b; the reflection mirror...

Embodiment 2

[0046] Such as Figure 1-3 As shown, this embodiment discloses a scanning imaging method based on frequency conversion laser, which adopts the scanning imaging system based on frequency conversion laser described in Embodiment 1, including the following steps:

[0047] S1: In the digital signal generator, set three analog signals and one digital pulse signal, the three analog signals are one frequency conversion signal and two sawtooth wave signals;

[0048] The frequency conversion signal drives the laser to generate frequency conversion intensity laser. The frequency conversion signal is defined as F(i)=sin[ω(i)×i+ψ], where in Indicates taking an integer, i and k are integers greater than 0, Feq and coe are rational numbers greater than 0, and ψ is a rational number greater than 0;

[0049] The two-way sawtooth wave signals are used as the scanning signals of the X axis and the Y axis to drive the two vibrating mirrors in the laser scanning device, and the sawtooth wave si...

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Abstract

The invention discloses a variable frequency laser-based scanning imaging system and method. The variable frequency laser-based scanning imaging system comprises a digital signal generator (1), a laser (2), a laser scanning device (3), a microscope module (4), a confocal detection module (5) and a digital acquisition card (6). Meanwhile, the invention also discloses variable frequency laser-basedscanning imaging method. By the variable frequency laser-based scanning imaging system, the space resolution of a fluorescent image can be improved, meanwhile, the fluorescent lifetime data of an imaging sample can be acquired, and a time-dimensional signal is provided for analysis of substance type.

Description

technical field [0001] The invention relates to a scanning imaging system, in particular to a scanning imaging system based on frequency conversion laser and a method thereof, belonging to the technical field of optical imaging. Background technique [0002] Optical imaging tools have gained prominence in scientific research in recent years. In order to obtain higher resolution, a large number of novel microscopic imaging methods have emerged. Among them, the laser confocal microscope system has become the first choice of scientific researchers due to its good imaging resolution, three-dimensional imaging capabilities and outstanding imaging mode expansion capabilities. [0003] In the confocal laser microscopy system, the improvement of resolution is an important issue. At present, the spatial resolution of laser confocal scanning has been improved by using double-beam dissipation excitation and spatial frequency modulation excitation. However, even with the introduction ...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N21/01
CPCG01N21/6402G01N21/01
Inventor赵磊
Owner杭州科洛码光电科技有限公司