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Application of egflam autoantibody detection reagent in the preparation of lung cancer screening kit

A technology of autoantibodies and kits, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of no EGFLAM protein, no lung cancer, etc., and achieve good application prospects, effective screening, and low patient harm.

Active Publication Date: 2020-06-02
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no relevant reports on EGFLAM protein autoantibodies, and there is no prior art related to lung cancer

Method used

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  • Application of egflam autoantibody detection reagent in the preparation of lung cancer screening kit
  • Application of egflam autoantibody detection reagent in the preparation of lung cancer screening kit
  • Application of egflam autoantibody detection reagent in the preparation of lung cancer screening kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Relationship between EGFLAM autoantibodies in plasma and lung cancer

[0027] 1. Clinical data

[0028] 30 lung cancer patients and 29 healthy controls were selected. The basic information is as follows:

[0029] Basic Information lung cancer patients healthy control number of people 30 29 age 49.5±5.7 42.0±8.9 male ratio 20(66.7％) 13(44.8％)

[0030] 2. Detection principle

[0031] HuProt TM EGFLAM protein is immobilized on the human protein custom chip (the EGFLAM protein used is a full-length protein, and the protein sequence number is Ensembl: ENSP00000423228). After incubation with serum, EGFLAM autoantibodies (mainly including IgG and IgM antibodies, and some Other types of antibodies) will bind to it, wash to remove unbound antibodies and other proteins, and then detect with anti-human IgM fluorescently labeled secondary antibody (cy5-labeled, red) and anti-human IgG fluorescent secondary antibody (cy3-labeled, g...

Embodiment 2

[0048] Embodiment 2 The composition of detection kit of the present invention and its application method

[0049] 1. Kit composition

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Abstract

The invention relates to the field of in vitro diagnostic reagents, in particular to application of an EGFLAM autoantibody detection reagent in preparation of a lung cancer screening kit. The invention, for the first time, finds that the autoantibody level of EGFLAM protein in the serum of lung cancer patients is significantly higher than those of healthy patients, and can realize effective screening of lung cancer by using the reagent of detecting the autoantibody level of EGFLAM protein to prepare the lung cancer screening kit.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic reagents, in particular to the use of EGFLAM autoantibody detection reagents in the preparation of lung cancer screening kits. Background technique [0002] Lung cancer is one of the most common malignant tumors in the world. Its morbidity and mortality are increasing year by year. Currently, the incidence rate ranks first in the world, which seriously threatens human health and life. [0003] Lung cancer is a disease that is good at concealment. It often shows clinical symptoms when the disease develops to an advanced stage. 70-80% of lung cancer patients are already in the middle or late stage when they are diagnosed with lung cancer symptoms. The best cure time, five-year survival rate is low. For patients with early lung cancer, timely treatment can greatly improve the 5-year survival rate and quality of life of the patients. Therefore, early diagnosis and effective screening of lung cance...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574
CPCG01N33/57423
Inventor 张立李为民
Owner WEST CHINA HOSPITAL SICHUAN UNIV