Application of pearl oyster small molecule peptide in skin wound repair
A technology of small molecular peptides and wound repair, applied in the field of biomedicine, can solve the problems of underutilized shellfish meat, and achieve the effect of facilitating proliferation and repair, good biological characteristics, and good affinity
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[0059] Example 1
[0060] An application of pearl oyster small molecule peptide in wound repair, characterized in that the pearl oyster small molecule peptide has a new purpose for repairing skin wounds;
[0061] The preparation method of the pearl oyster small molecule peptide includes the following steps:
[0062] S1. Preparation of pearl oyster hydrolysate;
[0063] The pearl oyster mantle is separated by pretreatment; the protease selected for the enzymatic hydrolysis of the pearl oyster mantle is animal protease, the added amount of protease is 4000 U / g, the pH value is 7.5, the enzymolysis temperature is 50°C, the enzymolysis time is 4 hours, 100°C After inactivating the enzyme for 10 minutes, centrifuge to collect the supernatant;
[0064] S2. The supernatant obtained in step S1 is selected with a ceramic membrane with a pore size of 0.05-0.5um, and after sufficient filtration, it is separated by ultrafiltration membranes with different molecular weight cut-offs to collect pepti...
Example Embodiment
[0086] Example 2
[0087] An application of pearl oyster small molecule peptide in wound repair, characterized in that the pearl oyster small molecule peptide has a new purpose for repairing skin wounds;
[0088] The preparation method of the pearl oyster small molecule peptide includes the following steps:
[0089] S1. Preparation of pearl oyster hydrolysate;
[0090] The pearl oyster mantle is separated by pretreatment; the protease selected for the enzymatic hydrolysis of the pearl oyster mantle is a neutral protease, the amount of protease added is 3000 U / g, the pH value is 6.5, the enzymolysis temperature is 45°C, the enzymolysis time is 3 hours, 100 After inactivating the enzyme at ℃ for 10 minutes, centrifuge to get the supernatant;
[0091] S2. The supernatant obtained in step S1 is selected with a ceramic membrane with a pore size of 0.05-0.5um, and after sufficient filtration, it is separated by ultrafiltration membranes with different molecular weight cut-offs to collect pep...
Example Embodiment
[0108] Example 3
[0109] An application of pearl oyster small molecule peptide in wound repair, characterized in that the pearl oyster small molecule peptide has a new purpose for repairing skin wounds;
[0110] The preparation method of the pearl oyster small molecule peptide includes the following steps:
[0111] S1. Preparation of pearl oyster hydrolysate;
[0112] The pearl oyster mantle is separated by pretreatment; the protease selected for the enzymatic hydrolysis of the pearl oyster mantle is alkaline protease, the added amount of protease is 5000 U / g, the pH value is 10, the enzymolysis temperature is 55°C, the enzymolysis time is 5 hours, 100 After inactivating the enzyme at ℃ for 10 minutes, centrifuge to get the supernatant;
[0113] S2. The supernatant obtained in step S1 is selected with a ceramic membrane with a pore size of 0.05-0.5um, and after sufficient filtration, it is separated by ultrafiltration membranes with different molecular weight cut-offs to collect pepti...
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