Label-free high-speed microscopic imaging method and device

Abstract

The invention discloses a label-free high-speed microscopic imaging method and device. The method comprises the steps of generating ultrashort pulse laser by using an ultrashort pulse laser light source; converting a beam of the ultrashort pulse laser into a Bessel beam with an extended axial focal depth, exciting a sample by using the Bessel beam and generating a harmonic signal with an axial focal depth in the sample through a nonlinear optical effect along a first preset direction; controlling the Bessel beam to carry out reciprocating motion at a preset horizontal scanning deflection anglein a second preset direction and controlling the sample to carry out longitudinal movement at a preset speed in a third preset direction to scan the Bessel beam in a sample plane and to excite the sample to generate the harmonic signal; and carrying out data reconstruction according to the harmonic signal to obtain a label-free high-speed and high-flux harmonic microscopic image. The method is capable of significantly improving the imaging speed and the data flux, can be applied to fast imaging of biological samples, such as pathological sections, and has a broad biomedical application prospect.

Description

technical field [0001] The invention relates to the field of optical microscopy technology, in particular to a label-free high-speed microscopic imaging method and device. Background technique [0002] Three-dimensional imaging of biological samples has important applications in many biomedical researches. As a routine method of clinical examination, pathological biopsy plays an important role in clinical diagnosis. At present, traditional hematoxylin-eosin staining (H&E staining) is usually used to stain biopsy sections in pathological biopsy, which is regarded as the "gold standard" for pathological detection. However, the H&E staining method requires complex pretreatment processes such as fixation, dehydration, wax immersion, dewaxing, sectioning, and staining, and then placing the sample under a microscope for observation and imaging, which cannot achieve real-time observation and diagnosis. In order to improve the efficiency of pathological detection and avoid the abo...

AI Technical Summary

Problems solved by technology

The lateral movement of the sample usually has a slow response speed, which wastes a lot of time, further reducing the imaging speed and data throughput of pathology slides

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