High performance liquid chromatography (HPLC) wavelength switching technology-based method for simultaneously measuring content of various constituents in san huang tablet

A technology of wavelength switching and Sanhuang tablets, which is applied in the field of simultaneous determination of the content of various components in Sanhuang tablets based on HPLC wavelength switching technology, which can solve the problems of poor operability, less determination components, and long analysis time, and improve detection sensitivity. , Simple operation, accurate measurement results

Inactive Publication Date: 2020-01-03
陕西起源农业科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the contents of emodin, chrysophanol, berberine hydrochloride, and baicalin are separately determined by high performance liquid chromatography (HPLC). The determination components are few, and the sample pretreatment is cumbersome, the analysis time is long, and the operability is not strong.
HPLC has the advantages of convenience and rapidity for the determination of chemical components in medicines. It has been reported that HPLC is used to simultaneously determine the active ingredients of three traditional Chinese medicines in Sanhuang Tablets, but there is no simultaneous quantitative analysis of the seven active ingredients in Sanhuang Tablets by HPLC wavelength conversion method. method of composition

Method used

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  • High performance liquid chromatography (HPLC) wavelength switching technology-based method for simultaneously measuring content of various constituents in san huang tablet
  • High performance liquid chromatography (HPLC) wavelength switching technology-based method for simultaneously measuring content of various constituents in san huang tablet
  • High performance liquid chromatography (HPLC) wavelength switching technology-based method for simultaneously measuring content of various constituents in san huang tablet

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Materials

[0028] 1.1 Instruments: LC-20AT high performance liquid chromatography (Shimadzu Corporation), including: quaternary gradient pump, online degasser, DAD detector, Chromeleon chromatography workstation, temperature-controlled autosampler; XS205 Mettler Electronic analytical balance (Mettler, Switzerland); KQ-500B ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); Milli-Q ultrapure water instrument (Millipore, USA); Millipore 0.45 μm membrane and filter ( American Millipore Company).

[0029] 1.2 Reagents

[0030] Reference substances baicalin (batch number: 110715-201720, purity: 98.5%), berberine hydrochloride (batch number: 110713-201613, purity: 86.8%), aloe-emodin (batch number: 110795-201710, purity: 98.3%), Rhein (batch number: 110757-201607, purity: 99.3%), emodin (batch number: 110756-201512, purity: 98.7%), chrysophanol (batch number: 110796-201621, purity: 99.2%), emodin methyl ether ( Batch number: 110758-201616, purity: 99.0%) wer...

Embodiment 2

[0045] Methodological investigation

[0046] 1. System suitability test

[0047] According to 2.3 chromatographic conditions in Example 1, take 10 μL of mixed reference substance solution and need testing solution respectively, and record the chromatogram. the result shows figure 1 The number of theoretical plates of the chromatographic peaks of the upper reference substance is greater than 5000, and the tailing factors are all between 0.95 and 1.05. figure 2 Above, the resolution of the chromatographic peak corresponding to the reference substance and its adjacent peaks is greater than 1.5. The chromatograms of mixed reference substance solution and need testing solution are shown in figure 1 with figure 2 .

[0048] 2. Linear relationship, limit of quantification and limit of detection

[0049] Accurately draw the mixed reference solution 1, 2, 4, 6, 8, and 10mL prepared in Example 1 respectively, put them in a 10mL volumetric flask, add methanol to constant volume, ...

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Abstract

The invention discloses a high performance liquid chromatography (HPLC) wavelength switching technology-based method for simultaneously measuring content of various constituents in san huang tablet, and relates to the technical field of drum detection method. The HPLC wavelength switching technology-based method comprises the following steps of simultaneously performing qualitative and quantitative analysis on seven types of constituents comprising baicalin, berberine hydrochloride, aloe-emodin, rhein, emodin and physcion; preparing a mixed contrast solution; preparing a sample solution; performing HPLC separation and detection; and performing qualitative and quantitative analysis on a sample. An HPLC wavelength switching technology is employed, detection is performed when baicalin wavelength is switched at 280 nanometers, detection is performed when berberine hydrochloride wavelength is switched at 264 nanometers, detection is performed when wavelengths of the aloe-emodin, the rhein,the emodin and the physcion are switched at 254 nanometers, an elution condition is in a way that mobility-phase A acetonitrile and mobility-phase B phosphate solution and is as follows: 15% of A and85% are contained of B when 0-10 minutes are passed, 25% of A and 75% of B are contained when 20 minutes are passed, 40% of A and 60% of B are contained when 30 minutes as passed, 55% of A and 45% B are contained when 42 minutes are passed, and 80% of A and 20% of B are contained when 50-60 minutes are passed. The HPLC wavelength switching technology-based method is simple to operate and is high in detection sensitivity and more accurate in detection result.

Description

technical field [0001] The invention relates to the technical field of detection methods for medicines, in particular to a method for simultaneously determining the contents of multiple components in Sanhuang Tablets based on HPLC wavelength switching technology. Background technique [0002] Sanhuang Tablets, made of rhubarb, berberine hydrochloride, and baicalin extract, has the functions of clearing away heat and detoxifying, purging fire and laxative. Indications for excessive heat in the Sanjiao, red eyes and swelling, sores in the mouth and nose, sore throat, vexation and thirst, yellow urine and constipation. Sanhuang Tablet is a classic Chinese patent medicine, which is very popular among the common people because of its definite curative effect, high quality and low price. In order to improve the quality of Sanhuang Tablets sold in the market, the State Food and Drug Administration has revised the drug several times, formulated supplementary inspection methods and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/74
CPCG01N30/02G01N30/06G01N30/74
Inventor 李浪阎岩林亮杜宏涛白朕卿毛仁俊舒志强李秀秀惠乐杨艳菠
Owner 陕西起源农业科技有限责任公司
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