High performance liquid chromatography (HPLC) wavelength switching technology-based method for simultaneously measuring content of various constituents in san huang tablet
A technology of wavelength switching and Sanhuang tablets, which is applied in the field of simultaneous determination of the content of various components in Sanhuang tablets based on HPLC wavelength switching technology, which can solve the problems of poor operability, less determination components, and long analysis time, and improve detection sensitivity. , Simple operation, accurate measurement results
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Embodiment 1
[0027] 1. Materials
[0028] 1.1 Instruments: LC-20AT high performance liquid chromatography (Shimadzu Corporation), including: quaternary gradient pump, online degasser, DAD detector, Chromeleon chromatography workstation, temperature-controlled autosampler; XS205 Mettler Electronic analytical balance (Mettler, Switzerland); KQ-500B ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); Milli-Q ultrapure water instrument (Millipore, USA); Millipore 0.45 μm membrane and filter ( American Millipore Company).
[0029] 1.2 Reagents
[0030] Reference substances baicalin (batch number: 110715-201720, purity: 98.5%), berberine hydrochloride (batch number: 110713-201613, purity: 86.8%), aloe-emodin (batch number: 110795-201710, purity: 98.3%), Rhein (batch number: 110757-201607, purity: 99.3%), emodin (batch number: 110756-201512, purity: 98.7%), chrysophanol (batch number: 110796-201621, purity: 99.2%), emodin methyl ether ( Batch number: 110758-201616, purity: 99.0%) wer...
Embodiment 2
[0045] Methodological investigation
[0046] 1. System suitability test
[0047] According to 2.3 chromatographic conditions in Example 1, take 10 μL of mixed reference substance solution and need testing solution respectively, and record the chromatogram. the result shows figure 1 The number of theoretical plates of the chromatographic peaks of the upper reference substance is greater than 5000, and the tailing factors are all between 0.95 and 1.05. figure 2 Above, the resolution of the chromatographic peak corresponding to the reference substance and its adjacent peaks is greater than 1.5. The chromatograms of mixed reference substance solution and need testing solution are shown in figure 1 with figure 2 .
[0048] 2. Linear relationship, limit of quantification and limit of detection
[0049] Accurately draw the mixed reference solution 1, 2, 4, 6, 8, and 10mL prepared in Example 1 respectively, put them in a 10mL volumetric flask, add methanol to constant volume, ...
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