Thermophilic facultative anaerobic microbial strain and application thereof

A facultative anaerobic and microbial agent technology, which is applied in the field of microbial enhanced oil recovery, can solve the problems of poor effect, lower crude oil viscosity and freezing point, low range of high pour point oil recovery, etc., to achieve enhanced oil recovery , enhance the effect of mobility

Active Publication Date: 2020-01-07
东方金道(北京)科技有限公司
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] For this reason, the embodiments of the present invention provide a novel thermophilic facultative anaerobic microbial strain and its application to solve the problem that the strains in the prior art a

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Thermophilic facultative anaerobic microbial strain and application thereof
  • Thermophilic facultative anaerobic microbial strain and application thereof
  • Thermophilic facultative anaerobic microbial strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1, the screening of thermophilic facultative anaerobic strain 64-1

[0037] Take 5 mL of oil well output fluid from a block in Liaohe Oilfield and inoculate it into a 200 mL Erlenmeyer flask containing 100 mL of enrichment medium, wherein the components of the enrichment medium include: corn steep liquor dry powder 0.4%, diammonium hydrogen phosphate 0.2 %, sodium nitrate 0.2%, yeast powder 0.05%, 150rpm, 70 ℃ constant temperature shaker culture for 7 days, take 10ml culture solution and put it into a 200mL Erlenmeyer flask with a capacity of 100mL enrichment medium for cultivation, repeat 5 Second-rate.

[0038]The obtained enrichment was serially diluted, and 0.1 mL was spread on a solid enrichment medium (containing 1% agar) plate medium, and cultured at 70°C. Then, typical colonies were picked and streaked 3 to 4 times repeatedly to obtain colonies of a single form.

Embodiment 2

[0039] Example 2, identification of thermophilic facultative anaerobic strain 64-1

[0040] A single colony on the agar solid medium in Example 1 was picked with a toothpick, and the 16S rDNA gene of the strain was amplified, using universal primers 27F and 1492R, as shown in Table 1. The reaction system is 50 μL: 2×Taq PCR mix 25 μL, 27F 1.0 μL, 1492R 1.0 μL, ddH 2 O 23.0 μL. The PCR reaction program was: denaturation at 94°C for 5min, 94°C for 30S, 57°C for 30S, 72°C for 1min and 30S, 30 cycles, extension at 72°C for 10min, and storage at 4°C.

[0041] Use 0.8% agarose gel electrophoresis to detect the PCR amplification product, sample: 3 μL PCR product + 1 μL Gel-Red, the electrophoresis condition is 150V 15min. Samples with positive test results were sent to BGI for sequencing.

[0042] Table 1

[0043]

[0044] The full-length sequence of the 16S rDNA gene of strain 64-1 was 1453 bps. According to the comparison results of GenBank, the similarity between the 16S rD...

Embodiment 3

[0045] Example 3, Morphological characteristics, physiological and biochemical experiments of thermophilic facultative anaerobic strain 64-1

[0046] 1. Test strains

[0047] The thermophilic facultative anaerobic strain 64-164-1CGMCC 1.16798 isolated in Example 1 of the present invention.

[0048] 2. Experimental method

[0049] Referring to the experimental method of "Bergey's Manual of Systematic Bacteriology", the Gram staining, cell size, shape, growth temperature, pH and sodium chloride tolerance range of the tested strains were detected, and the anaerobic characteristics of the strains were detected by the puncture test.

[0050] 3. Experimental results

[0051] Morphological characteristics of the tested strains: Gram staining was negative. Such as figure 2 As shown, the cells under the scanning electron microscope are short rod-shaped, without flagella, and the cell size is 0.6-0.8 μm×1.0-1.2 μm.

[0052] Such as image 3 As shown, the physiological and biochem...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Surface tensionaaaaaaaaaa
Login to view more

Abstract

The embodiments of the invention disclose a thermophilic anaerobic microbial strain 64-1 and an application thereof. The strain has a Latin name of Phaeospirillum sp., is collected in General Microorganisms Center of China Committee for Culture Collection of Microorganisms on August 6, 2019, has a collection number of CGMCC 1. 16798 and is collected at 3#, courtyard 1#, west Beichen road, Chaoyangdistrict, Beijing, China. A 16S rDNA gene of the strain 64-1 has a full-length sequence of 1453bps; and shown by carrying out an analysis according to a comparison result of GenBank, a 16S rDNA genesequence of the strain and a 16S rDNA gene sequence of Telmatospirillum siberiense 26-4b1(T) have the degree of similarity of 92.47%. The thermophilic facultative anaerobic strain 64-1 CGMCC 1. 16798disclosed by the embodiments of the invention has a very good degradation action on high pour point oil, the surface tension of fermentation broth can be lowered by a biological surfactant generated by the strain, and thus, the strain has very good emulsifying and dispersing capability to the high pour point oil. The strain and cultures thereof can be used for increasing the recovery ratio of thehigh pour point oil.

Description

[0001] This application claims the priority of the Chinese patent application submitted to the China Patent Office on July 08, 2019, with the application number 201910611014.X, and the application name is a strain of thermophilic facultative anaerobic microorganisms and its application. References are incorporated in this application. technical field [0002] The embodiment of the present invention relates to the application of a novel thermophilic facultative anaerobic microbial strain in high pour point oil recovery, which belongs to the technical category of microbial enhanced crude oil recovery. The embodiment of the present invention relates to using the microbial strain and bacterial agent to act on high pour point oil, by changing the physical and chemical properties of crude oil, enhancing the fluidity and displaceability of crude oil, and improving the recovery rate of crude oil. The bacterial strains and bacterial agents disclosed in the embodiments of the present in...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C09K8/582C12R1/01
CPCC09K8/582C12N1/20C12N1/205C12R2001/01
Inventor 王小通
Owner 东方金道(北京)科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap