A kind of extraction method of high-purity mogroside V

Abstract

The invention provides a method for extracting high-purity mogroside V, which comprises the following steps: freezing high-temperature pretreatment, normal temperature and ultra-high pressure extraction, ZTC-II clarifier clarification, 10 micron C 18 Preparative column purification, concentration and drying etc. The invention reduces the damage to the natural active ingredients of mogroside V through freezing high temperature treatment and normal temperature ultra-high pressure treatment, and extracts mogroside V with a purity of up to 99.9%. At the same time, compared with the traditional heating and reflux extraction method, the extraction efficiency is significantly improved and the extraction time is shortened. Time, but also save energy, to achieve continuous large-scale production.

Description

technical field

[0001] The invention relates to the field of natural plant extraction, in particular to a method for extracting high-purity mogroside V. Background technique

[0002] Luo Han Guo is one of the famous specialties in Guangxi, it is the ripe fruit of the genus Siraitia grosvenorii (Swingle) C. Jeffery of Cucrubitaceae. There are large areas of cultivation in Yongfu, Lingui, Longsheng and other counties in Guangxi, and the yield is considerable. It is one of the main sources of supply for foreign trade of Luo Han Guo. Monk fruit has a long history of medicinal use. In addition to being used as a formula, it is often used as a raw material to produce Chinese patent medicine products. It is one of the traditional Chinese medicinal materials exported in my country. Luo Han Guo contains 16 kinds of essential trace elements and a wide range of elements, rich in vitamin C, vitamin E, essential fatty acids such as linoleic acid, oleic acid, palmitic acid and other esse...

Examples

Embodiment 1

[0028] 1) Pretreatment: After freezing the raw material Luo Han Guo at -70°C for 10 minutes, it was immediately heated at 95°C for 10 seconds.

[0029] 2) Extraction: take distilled water as the extraction solvent, the ratio of material to liquid is 1:10g / mL, crush, soak after crushing, the condition is 25 ℃, soak for 5h, transfer into polyethylene plastic bag after stirring, soak under the pressure of 250KPa After 5 min of extraction, the supernatant was collected by centrifugation to obtain the extract.

[0030] 3) Clarification:

[0031] Preparation of ZTC-II natural clarifying agent clarification solution: 1 g of component A, firstly stir into a paste with a small amount of distilled water, then add the required amount of deionized water, swell for 24 hours, stir to make a 1% solution; 1 g of component B , dissolve with a small amount of 1% acetic acid solution, and stir into a paste, add a sufficient amount of 1% acetic acid solution, swell for 24h, stir to make a 1% sol...

Embodiment 2

[0036] 1) Pretreatment: After freezing the raw material Luo Han Guo at -50°C for 30 minutes, immediately heated at 100°C for 30 seconds;

[0037] 2) Extraction: take distilled water as the extraction solvent, the ratio of material to liquid is 1:30g / mL, crush, soak after crushing, the condition is 30°C, soak for 8h, stir and transfer into a polyethylene plastic bag, soak under the pressure of 400KPa After 10 min of extraction, the supernatant was collected by centrifugation to obtain the extract.

[0038] 3) Clarification:

[0039] Preparation of ZTC-II natural clarifying agent clarification solution: 1 g of component A, firstly stir into a paste with a small amount of distilled water, then add the required amount of deionized water, swell for 24 hours, stir to make a 1% solution; 1 g of component B , dissolve with a small amount of 1% acetic acid solution, and stir into a paste, add a sufficient amount of 1% acetic acid solution, swell for 24h, stir to make a 1% solution.

...

Embodiment 3

[0044] 1) Pretreatment: After freezing the raw material Luo Han Guo at -60°C for 20 minutes, it was immediately heated at 98°C for 20 seconds.

[0045] 2) Extraction: take distilled water as the extraction solvent, the ratio of material to liquid is 1:18g / mL, crush, soak after crushing, the condition is 28°C, soak for 6h, transfer into polyethylene plastic bag after stirring, and soak under the pressure of 350KPa After 8 min of extraction, the supernatant was collected by centrifugation to obtain the extract.

[0046] 3) Clarification:

[0047] Preparation of ZTC-II natural clarifying agent clarification solution: 1 g of component A, firstly stir into a paste with a small amount of distilled water, then add the required amount of deionized water, swell for 24 hours, stir to make a 1% solution; 1 g of component B , dissolve with a small amount of 1% acetic acid solution, and stir into a paste, add a sufficient amount of 1% acetic acid solution, swell for 24h, stir to make a 1%...

technical field

[0001] The invention relates to the field of natural plant extraction, in particular to a method for extracting high-purity mogroside V. Background technique

[0002] Luo Han Guo is one of the famous specialties in Guangxi, it is the ripe fruit of the genus Siraitia grosvenorii (Swingle) C. Jeffery of Cucrubitaceae. There are large areas of cultivation in Yongfu, Lingui, Longsheng and other counties in Guangxi, and the yield is considerable. It is one of the main sources of supply for foreign trade of Luo Han Guo. Monk fruit has a long history of medicinal use. In addition to being used as a formula, it is often used as a raw material to produce Chinese patent medicine products. It is one of the traditional Chinese medicinal materials exported in my country. Luo Han Guo contains 16 kinds of essential trace elements and a wide range of elements, rich in vitamin C, vitamin E, essential fatty acids such as linoleic acid, oleic acid, palmitic acid and other esse...

Examples

Embodiment 1

[0028] 1) Pretreatment: After freezing the raw material Luo Han Guo at -70°C for 10 minutes, it was immediately heated at 95°C for 10 seconds.

[0029] 2) Extraction: take distilled water as the extraction solvent, the ratio of material to liquid is 1:10g / mL, crush, soak after crushing, the condition is 25 ℃, soak for 5h, transfer into polyethylene plastic bag after stirring, soak under the pressure of 250KPa After 5 min of extraction, the supernatant was collected by centrifugation to obtain the extract.

[0030] 3) Clarification:

[0031] Preparation of ZTC-II natural clarifying agent clarification solution: 1 g of component A, firstly stir into a paste with a small amount of distilled water, then add the required amount of deionized water, swell for 24 hours, stir to make a 1% solution; 1 g of component B , dissolve with a small amount of 1% acetic acid solution, and stir into a paste, add a sufficient amount of 1% acetic acid solution, swell for 24h, stir to make a 1% sol...

Embodiment 2

[0036] 1) Pretreatment: After freezing the raw material Luo Han Guo at -50°C for 30 minutes, immediately heated at 100°C for 30 seconds;

[0037] 2) Extraction: take distilled water as the extraction solvent, the ratio of material to liquid is 1:30g / mL, crush, soak after crushing, the condition is 30°C, soak for 8h, stir and transfer into a polyethylene plastic bag, soak under the pressure of 400KPa After 10 min of extraction, the supernatant was collected by centrifugation to obtain the extract.

[0038] 3) Clarification:

[0039] Preparation of ZTC-II natural clarifying agent clarification solution: 1 g of component A, firstly stir into a paste with a small amount of distilled water, then add the required amount of deionized water, swell for 24 hours, stir to make a 1% solution; 1 g of component B , dissolve with a small amount of 1% acetic acid solution, and stir into a paste, add a sufficient amount of 1% acetic acid solution, swell for 24h, stir to make a 1% solution.

...

Embodiment 3

[0044] 1) Pretreatment: After freezing the raw material Luo Han Guo at -60°C for 20 minutes, it was immediately heated at 98°C for 20 seconds.

[0045] 2) Extraction: take distilled water as the extraction solvent, the ratio of material to liquid is 1:18g / mL, crush, soak after crushing, the condition is 28°C, soak for 6h, transfer into polyethylene plastic bag after stirring, and soak under the pressure of 350KPa After 8 min of extraction, the supernatant was collected by centrifugation to obtain the extract.

[0046] 3) Clarification:

[0047] Preparation of ZTC-II natural clarifying agent clarification solution: 1 g of component A, firstly stir into a paste with a small amount of distilled water, then add the required amount of deionized water, swell for 24 hours, stir to make a 1% solution; 1 g of component B , dissolve with a small amount of 1% acetic acid solution, and stir into a paste, add a sufficient amount of 1% acetic acid solution, swell for 24h, stir to make a 1%...