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Degradable anti-tumor nano-medicine based on limotherapy, as well as preparation method and application thereof

A nano-drug, starvation therapy technology, applied in anti-tumor drugs, biochemical equipment and methods, drug combinations, etc., can solve problems such as poor tumor accumulation and retention, affecting treatment effects, etc. Metabolism, the effect of fast degradation rates

Active Publication Date: 2020-01-14
YANSHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Smaller nanoparticles can achieve rapid renal clearance, but their tumor accumulation and retention are poor, thereby affecting the therapeutic effect

Method used

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  • Degradable anti-tumor nano-medicine based on limotherapy, as well as preparation method and application thereof
  • Degradable anti-tumor nano-medicine based on limotherapy, as well as preparation method and application thereof
  • Degradable anti-tumor nano-medicine based on limotherapy, as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Dissolve octreotide acetate (Shanghai Taishi Biotechnology Co., Ltd.) in hydrochloric acid (Yongfei Chemical Factory) solution to prepare octreotide acetate hydrochloric acid solution with a concentration of 0.3mM and a pH value of 1, and prepare AgNO with a concentration of 0.9mM respectively. 3 (Beijing Beihua Fine Chemicals Co., Ltd.) solution and ascorbic acid (Beijing Baierdi Biotechnology Co., Ltd.) solution with a mass concentration of 3%, they were mixed with a volume ratio of 1:1, ultrasonic treatment was performed for 30s, and ultrasonic power was 90W. A silver colloid solution was obtained. Configure AgNO at a concentration of 7mM 3 (Beijing Beihua Fine Chemicals Co., Ltd.) solution, mixed with the above-mentioned octreotide acetate solution and silver colloid solution in a volume ratio of 1:1:3, placed in a digital display double-layer air bath constant temperature oscillator, at 200rpm, 20 Incubate at ℃ for 20 h. NaBH with a concentration of 80mM 4 (Beij...

Embodiment 2

[0042] Dissolve octreotide acetate (Shanghai Taishi Biotechnology Co., Ltd.) in hydrochloric acid (Yongfei Chemical Factory) solution to prepare octreotide acetate hydrochloric acid solution with a concentration of 0.3mM and a pH value of 1, and prepare AgNO with a concentration of 0.9mM respectively. 3 (Beijing Beihua Fine Chemicals Co., Ltd.) solution and ascorbic acid (Beijing Baierdi Biotechnology Co., Ltd.) solution with a mass concentration of 3%, they were mixed with a volume ratio of 1:1, ultrasonic treatment was performed for 30s, and ultrasonic power was 90W. A silver colloid solution was obtained. Configure AgNO at a concentration of 7mM 3 (Beijing Beihua Fine Chemicals Co., Ltd.) solution, mixed with the above-mentioned octreotide acetate solution and silver colloid solution in a volume ratio of 1:1:3, placed in a digital display double-layer air bath constant temperature oscillator, at 200rpm, 20 Incubate at ℃ for 20 h. A NaBH4 (Beijing Zhongsheng Huateng Techno...

Embodiment 3

[0046] Dissolve octreotide acetate (Shanghai Taishi Biotechnology Co., Ltd.) in hydrochloric acid (Yongfei Chemical Factory) solution to prepare octreotide acetate hydrochloric acid solution with a concentration of 0.3mM and a pH value of 1, and prepare AgNO with a concentration of 0.9mM respectively. 3 (Beijing Beihua Fine Chemicals Co., Ltd.) solution and ascorbic acid (Beijing Baierdi Biotechnology Co., Ltd.) solution with a mass concentration of 3%, they were mixed with a volume ratio of 1:1, ultrasonic treatment was performed for 30s, and ultrasonic power was 90W. A silver colloid solution was obtained. Configure AgNO at a concentration of 7mM 3 (Beijing Beihua Fine Chemicals Co., Ltd.) solution, mixed with the above-mentioned octreotide acetate solution and silver colloid solution in a volume ratio of 1:1:3, placed in a digital display double-layer air bath constant temperature oscillator, at 200rpm, 20 Incubate at ℃ for 20 h. NaBH with a concentration of 80mM 4 (Beij...

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Abstract

The invention discloses a degradable anti-tumor nano-medicine based on limotherapy, as well as a preparation method and application thereof, and belongs to the technical field of nano-medicines. The preparation method comprises the following steps: dissolving dopamine hydrochloride in a Tris-HCl buffer solution, adding glucose oxidase into the mixture, and performing quick stirring; filtering themixture through a 0.45[mu]m water filter membrane, continuing to quickly stir the solution after filtering, and filtering the mixture through the 0.45[mu]m water filter membrane; centrifuging the secondary filtered liquid with a silver cubic solution prepared isometrically, performing precipitating and mixing, performing ultrasonic and standing treatment on the mixed solution, and performing centrifuging to obtain precipitation; resuspending the precipitation in a PBS buffer solution to obtain a glucose oxidase-loaded silver nano-cube. The particle glucose oxidase has a loading capacity of 10-25 percent and an average particle size of 100-150nm, is acidic in a tumor part and can be degraded in a hydrogen peroxide environment, and can achieve the EPR effect and a quick internal cleaning function; and an excellent tumor treatment effect is showed by combining the limotherapy of consuming glucose in the tumor part by the glucose oxidase and a photothermal therapy of the nano-silver cube.

Description

technical field [0001] The invention relates to a degradable anti-tumor nano drug based on starvation therapy, a preparation method and application thereof, and belongs to the technical field of nano drugs. Background technique [0002] Compared with other diseases, cancer has a high incidence rate and low cure rate, and now it has surpassed cardiovascular disease to become the biggest threat to human health in the world. At present, there are about 14.1 million new cancer cases (excluding skin cancers other than melanoma) in the world on average every year, resulting in about 8.8 million deaths, accounting for nearly 15.7% of the total annual global deaths, and the number of new cases is expected to increase in 2035 to 24 million. Although traditional treatment modes such as chemotherapy and radiotherapy can achieve certain therapeutic effects, they often have side effects such as hair loss and low immunity. [0003] Since the 21st century, with the development of nanosci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K9/51A61K38/08A61K38/44A61K47/34A61P35/00
CPCA61K9/5146A61K38/08A61K38/443A61K41/0052A61P35/00C12Y101/03004A61K2300/00
Inventor 高大威程欣杨梦雪赫雅倩
Owner YANSHAN UNIV
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