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Droplet transfer method and device, cleaning method of droplet transportation paths

A transport path and droplet technology, applied in chemical instruments and methods, laboratory containers, laboratory utensils, etc., can solve problems such as poor and cross-contamination cleaning effects, and achieve the effect of solving poor cleaning effects

Active Publication Date: 2020-01-17
INST OF MICROELECTRONICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The embodiment of the present application provides a liquid droplet transmission method and device, and a liquid droplet transportation path cleaning method, to at least solve the problem of cross-contamination in the liquid droplet transmission path in the related art and poor cleaning effect of cleaning cross-contamination points technical problem

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  • Droplet transfer method and device, cleaning method of droplet transportation paths
  • Droplet transfer method and device, cleaning method of droplet transportation paths
  • Droplet transfer method and device, cleaning method of droplet transportation paths

Examples

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B example 1

[0109]B. Case 1: Multiplex In Vitro Diagnostics

[0110] Here the proposed method for bioassays, multiplex in vitro diagnostics on human body fluids, is evaluated. Three human body fluids were sampled: urine, serum, and plasma, and distributed to a digital microfluidic biochip. Glucose and lactate measurements were performed on each body fluid.

[0111] The droplet transport problem for this measurement is decomposed into 11 subproblems. These subproblems are solved in turn by attempting to identify a set of vertex-disjoint or edge-disjoint droplet paths and minimizing the number of cells used by these paths subject to all constraints. For the path length constraint, the length of each droplet path should not exceed 20 electrodes. Therefore, the path length constraint T d Equivalent to 20 electrodes.

[0112] Here, subproblem 3 is used to illustrate the previously proposed method for synchronizing disjoint paths and cleaning operations. Such as Figure 6 As shown in (a)...

C example 2

[0130] C. Case 2: Protein Assay

[0131] The proposed method was then evaluated for a protein assay (already implemented on a digital microfluidic assay chip), whose routing problem was decomposed into 127 subproblems.

[0132] In subproblem 82, there are five 2-pin nets used to deliver functional droplets, Dlt 23 run simultaneously, such as Figure 8 (a). Such as Figure 8 As shown in (b), droplet D 1 Follow the path from DsB 28 The buffer allocator is sent to the chip storage unit S 4 ; droplet D 2 From Dlt 29 The diluent is sent to the chip storage unit S 2 ; droplet D 3 From Dlt 20 The diluter is sent to the chip storage unit S 1 ; droplet D 4 from DsB 30 The buffer allocator is sent to the chip storage unit S 3 ; droplet D 5 from DsB 24 The buffer allocator passed to Dlt 24 diluter. Four functional droplets D 2 -D 5 paths intersect at four cross-contamination points. S i,j is D i and D j The cross-contamination site of the route.

[0133] Figure...

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Abstract

The application discloses a droplet transfer method and device and a cleaning method of droplet transportation paths. The method comprises the following steps of obtaining an undirected graph of a two-dimensional microfluidic array, wherein the undirected graph comprises a vertex set and an edge set, each vertex in the vertex set represents an electrode in the microfluidic array, and an edge between two adjacent vertices represents a path between the two adjacent vertexes; determining mutually disjoint vertexes or mutually disjoint edges in the undirected graph; determining droplet transport paths based on mutually disjoint vertexes or mutually disjoint edges, wherein the droplet transport paths in each group of droplet transport paths are mutually disjoint; and transferring the droplets along a droplet transportation path. Through the transfer method and device and the cleaning method disclosed by the application, the cross contamination problem existent in the droplet transfer path and the bad cleaning effect when cleaning the cross contamination point in the related technology can be solved.

Description

technical field [0001] The present application relates to the field of digital microfluidics, in particular, to a liquid droplet transport method and device, and a liquid droplet transport path cleaning method. Background technique [0002] Digital microfluidics is an emerging technology capable of processing fluids on a chip. This technology advances the automation of biochemical laboratory procedures. The digital microfluidic biochip reduces the consumption speed of samples and reagents, thereby ensuring the continuous availability of sample sampling and analysis, thereby completing real-time biochemical analysis. Electrowetting is used to manipulate nanoliter droplets ("cells") in a "digital" manner under the control of a clock on a two-dimensional array of electrodes. At present, enzyme assays, DNA sequencing, cell experiments, and immunoassays have been successfully implemented on digital microfluidic chips. Digital microfluidic samples used in clinical diagnosis inc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00
CPCB01L3/502784B01L2200/12B01L2200/141
Inventor 赵阳陈岚冯燕
Owner INST OF MICROELECTRONICS CHINESE ACAD OF SCI