Application of SYNPO gene methylation to diagnostic agent of asthenozoospermia and kit

A diagnostic kit and methylation technology, applied in recombinant DNA technology, microbial determination/examination, biochemical equipment and methods, etc., can solve the problems of less methylated regions and accurate diagnosis of subjects.

Inactive Publication Date: 2020-01-17
深圳市龙华区人民医院
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Problems solved by technology

[0004] However, there are very few reported methylated regions that can be used as molecular markers of asthenospermia, and it is difficult to accurately diagnose whether the subject has asthenospermia. Therefore, it is necessary to identify more methylated regions related to asthenospermia. basement region, thus providing more basis for the application of subsequent diagnosis and treatment

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  • Application of SYNPO gene methylation to diagnostic agent of asthenozoospermia and kit
  • Application of SYNPO gene methylation to diagnostic agent of asthenozoospermia and kit
  • Application of SYNPO gene methylation to diagnostic agent of asthenozoospermia and kit

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Embodiment Construction

[0015] The principles and features of the present invention will be described below with reference to the accompanying drawings. The examples cited are only used to explain the present invention, and are not used to limit the scope of the present invention.

[0016] 1. Separation of sperm

[0017] Seven volunteers with asthenospermia (group A) and eight healthy volunteers (group N) were recruited. Sperm samples were obtained from the volunteers to conduct genome-wide methylation level sequencing research. Use Sperm Class Analyzer to perform computer-aided sperm analysis (CASA), and perform Diff-Quik rapid staining method to evaluate sperm morphology. Separate sperm cells with different viability by the following method: Centrifuge at 300×g for 20 minutes to prepare non-continuous density gradient fractions (divided into 47.5, 57, 76 and 95% concentration layers), from 95% concentration layers (fractions) a) Collect high viability sperm cells, collect low viability sperm cells from...

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Abstract

The invention relates to application of the methylation level of a human SYNPO gene promoter region to preparation of a detection agent for detecting sperm motility. The human SYNPO gene promoter region is located in a region corresponding to sites 149978442-149981142 of the human fifth chromosome. Analysis and studies based on the results of methylation sequencing of the whole genome are performed, through verification, it is found that whether all methylation sites of the human SYNPO gene promoter region are methylated or not between patients with asthenozoospermia and healthy personnel is significantly different and can be used as a molecular marker to diagnose whether a subject has asthenozoospermia or not. When not all methylation sites in the region are methylated, the subject is more likely to have asthenozoospermia. The molecular marker can provide auxiliary evaluation information for the diagnosis of asthenozoospermia, and identify whether the subject has asthenozoospermia ornot combined with other diagnostic indicators.

Description

Technical field [0001] The invention relates to the field of human reproductive health, and more particularly, to the application of the methylation level of the promoter region of the human SYNPO gene in the preparation of a diagnostic agent for asthenospermia, and a diagnostic kit for asthenospermia. Background technique [0002] More than 40% of infertile men have been tested for asthenospermia. The etiology of asthenospermia is multifactorial. For example, it may be closely related to endocrine disorders, environmental factors, life experience, and even getting older. Studies on gene knockout and mutation in mice have shown that some gene mutations are related to changes in sperm motility, but these genes have been shown to have nothing to do with sperm motility in human studies. Although nearly 50% of infertility cases are considered to be caused by genetic defects, in some cases of male infertility, genetic factors do not seem to be the main factor. A growing body of evid...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/154
Inventor 杜野薛兴奎古艳丽陈晓玲马晓瑞
Owner 深圳市龙华区人民医院
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