Serum protein marker for early screening and diagnosis of breast cancer, kit and detection method
A technology of serum protein and detection method, applied in the field of biomedicine, can solve the problems of less than 20% sensitivity and low diagnostic value, and achieve the effects of assisting clinical diagnosis, low cost and strong specificity
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[0039] 1 Preparation of serum samples
[0040] 1.1 Serum samples for protein chip experiments
[0041] Patients with primary breast cancer (breast cancer diagnosed by pathology) were collected at Beijing You'an Hospital and the First Affiliated Hospital of Zhengzhou University, with patient consent and approval by the Institutional Review Board and Hospital Ethics Committee. For all samples, 5-10mL of whole blood was collected in red-headed blood collection tubes. After standing at room temperature for 2 hours, centrifuge at 1000g for 15 minutes, take the supernatant, divide each sample into several copies, label them, and store them in a low-temperature refrigerator at -80°C. , avoid repeated freezing and thawing.
[0042] According to the epidemiological analysis, the sera of 50 cases of primary breast cancer and 27 cases of normal control sera from You'an Hospital in the same period of physical examination were finally collected for preliminary chip screening. The 50 pati...
Embodiment
[0105] A serum protein marker for early screening and diagnosis of breast cancer. The serum protein marker is a combination of proteins encoded by p62, PTCH1, BRCA2, HRAS, FUBP1 and GATA3 genes. The protein encoded by the p62 gene has the amino acid sequence shown in SEQ ID NO.3; the protein encoded by the PTCH1 gene has the amino acid sequence shown in SEQ ID NO.4; the protein encoded by the BRCA2 gene has the amino acid sequence shown in SEQ ID NO.4; The amino acid sequence shown in ID NO.5; the protein encoded by the HRAS gene has the amino acid sequence shown in SEQ ID NO.6; the protein encoded by the FUBP1 gene has the amino acid sequence shown in SEQ ID NO.7; The protein encoded by the GATA3 gene has the amino acid sequence shown in SEQ ID NO.8.
[0106] A kit, including serum protein markers for early screening and diagnosis of breast cancer, said serum protein markers are coated on a solid phase carrier, said solid phase carrier is a groove made of polyvinyl chloride ...
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