Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Serum protein marker for early screening and diagnosis of breast cancer, kit and detection method

A technology of serum protein and detection method, applied in the field of biomedicine, can solve the problems of less than 20% sensitivity and low diagnostic value, and achieve the effects of assisting clinical diagnosis, low cost and strong specificity

Active Publication Date: 2020-01-21
ZHENGZHOU UNIV
View PDF11 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Subsequently, the anti-p62 autoantibodies were detected in the serum of patients with liver cancer, liver cirrhosis, chronic hepatitis and normal people in small samples. The results showed that the specificity of this autoantibody was very high, but the sensitivity could not reach 20%.
Obviously, if the anti-p62 autoantibody is used alone as a marker for the diagnosis of liver cancer, the diagnostic value is low

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Serum protein marker for early screening and diagnosis of breast cancer, kit and detection method
  • Serum protein marker for early screening and diagnosis of breast cancer, kit and detection method
  • Serum protein marker for early screening and diagnosis of breast cancer, kit and detection method

Examples

Experimental program
Comparison scheme
Effect test

experiment example

[0039] 1 Preparation of serum samples

[0040] 1.1 Serum samples for protein chip experiments

[0041] Patients with primary breast cancer (breast cancer diagnosed by pathology) were collected at Beijing You'an Hospital and the First Affiliated Hospital of Zhengzhou University, with patient consent and approval by the Institutional Review Board and Hospital Ethics Committee. For all samples, 5-10mL of whole blood was collected in red-headed blood collection tubes. After standing at room temperature for 2 hours, centrifuge at 1000g for 15 minutes, take the supernatant, divide each sample into several copies, label them, and store them in a low-temperature refrigerator at -80°C. , avoid repeated freezing and thawing.

[0042] According to the epidemiological analysis, the sera of 50 cases of primary breast cancer and 27 cases of normal control sera from You'an Hospital in the same period of physical examination were finally collected for preliminary chip screening. The 50 pati...

Embodiment

[0105] A serum protein marker for early screening and diagnosis of breast cancer. The serum protein marker is a combination of proteins encoded by p62, PTCH1, BRCA2, HRAS, FUBP1 and GATA3 genes. The protein encoded by the p62 gene has the amino acid sequence shown in SEQ ID NO.3; the protein encoded by the PTCH1 gene has the amino acid sequence shown in SEQ ID NO.4; the protein encoded by the BRCA2 gene has the amino acid sequence shown in SEQ ID NO.4; The amino acid sequence shown in ID NO.5; the protein encoded by the HRAS gene has the amino acid sequence shown in SEQ ID NO.6; the protein encoded by the FUBP1 gene has the amino acid sequence shown in SEQ ID NO.7; The protein encoded by the GATA3 gene has the amino acid sequence shown in SEQ ID NO.8.

[0106] A kit, including serum protein markers for early screening and diagnosis of breast cancer, said serum protein markers are coated on a solid phase carrier, said solid phase carrier is a groove made of polyvinyl chloride ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Sensitivityaaaaaaaaaa
Sensitivityaaaaaaaaaa
Sensitivityaaaaaaaaaa
Login to View More

Abstract

The invention discloses a serum protein marker for early screening and diagnosis of breast cancer, which belongs to the technical field of biomedicine. The serum protein marker for early screening anddiagnosis of the breast cancer is any one or a combination of two or more of the proteins encoded by the CEBPA, RalA, p62, PTCH1, BRCA2, HRAS, FUBP1, GATA3, FGFR3, ALK, HISTIH3B or p53 genes. The invention also discloses a kit and a detection method including the serum protein marker for early screening and diagnosis of the breast cancer. Based on the role played by cancer driver genes in the genesis and development of the tumor, the invention customizes a human protein chip encoded by 138 cancer driver genes, which contains 180 human-derived recombinant proteins. Firstly, an early detectionserum marker of the breast cancer is first screened through the protein chip; then verification is performed by using the ELISA indirect method; and finally, a group of breast cancer serum protein marker that can be used for early screening and diagnosis of the breast cancer is screened out. The area under the ROC curve of the combined diagnosis of breast cancer reaches 0.886; the 95% CI is 0.847to 0.925; and when the specificity is ensured to be 92.2%, the sensitivity is 67.9%, and the consistency rate reaches 80.1%.

Description

technical field [0001] The invention belongs to the technical field of biomedicine. Background technique [0002] Breast cancer is one of the most common malignant tumors in the world. In most Asian countries including China, it ranks first in female cancer mortality. Its incidence rate accounts for about 11.6% of other malignant tumors in the whole body. The increase in incidence rate is obviously higher than that in western countries, which seriously threatens people's life and health. The onset of breast cancer is hidden, and most patients have already entered the advanced stage of breast cancer when they are first diagnosed, thus losing the opportunity for treatment. The main reason is that breast cancer is mostly a painless mass in the early stage, which is not easy to attract attention and does not have typical symptoms and signs in the early stage, or the tumor is small and difficult to detect by imaging examination. At present, the latest early detection methods fo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/536G01N33/574G01N21/31
CPCG01N33/536G01N33/57415G01N21/31
Inventor 张建营叶华王鹏王晓史健翔代丽萍邱翠鹏黎天东
Owner ZHENGZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com