131 results about "EDARADD" patented technology

The invention provides methods and materials that use observation of DNA characteristics to obtain information relating to the age of individuals. The instant disclosure identifies 88 sites in or near 80 genes for which the degree of cytosine methylation in epithelial and / or white blood cells is significantly correlated with age. In illustrative embodiments of the invention, cytosine methylation patterns the promoters of the EDARADD, TOMILI, and NPTX2 genes are used to predict the age of an individual with a high degree of accuracy.

The invention discloses a mutant human plasminogen kringle5 gene mK5 and an amplification primer of the mK5 gene, the mutant human plasminogen kringle5 gene modified by adding glutathione-S-transferase before the gene mK5, and a method for preparing protein mK5 recombinant protein of two gene codes, glutathioneS transferase (GST)-mK5 fusion protein and two proteins, wherein the mK5 gene and the GST-mK 5 gene can be applied to preparing medicaments for treating angiogenesis diseases. By the invention, the number of exogenous amino acid in the recombinant K5 protein molecules obtained by a gene engineering method is remarkably reduced, the K5 bioactivity of the obtained mK5 is improved, while the mK5 activity of the GST-mK5 fusion protein is maintained, the stability and water solubility of the protein are improved, the purification steps of an expressed product are simplified, and the purity of the product is improved.

The invention discloses preparation of novel duck reovirus recombinant sigma B protein antigen, and application of the novel duck reovirus recombinant sigma B protein antigen in detection of a novel duck reovirus antibody. With RNA (ribonucleic acid) of the novel duck reovirus (NDRV) as a template, a nucleotide complete sequence of an NDRV S3 gene coding region can be amplified through a reverse-translation polymerase-chain reaction, is directionally subcloned to a fusion expression vector of the prokaryotic expression vector pET-30a-C(+) of escherichia coli, and transformed into the host cell of the Escherichia coli BL21 (DE3), and is subjected to inducible expression with IPTG (isopropyl-beta-d-thiogalactoside), purification of Ni-NTA Agarose, inclusion body refolding to obtain the expressed NDRV alpha B recombinant protein antigen. The expression mode of the protein is fusion protein (His-alpha B) with molecular weight being about 44kU; and immunoblotting assays prove that the protein has immunoreactivity similar to natural protein..

The invention relates to the method of gene fixed-point mutation which is a modified overlap extension PCR method and the mutated gene. The process includes the fragment composition, the double mixing, the prepared extension, the whole DNA composition and after extension. The method is detected by the synzyme gene sam1 from the SAM of the Saccharomyces cerevisiae and gets the mutated gene modified by the rare coden. The SAM synzyme encoding by the gene can express in the yeast and improves the yield of the intracellular SAM. The invention can reach the many fixed-point mutations in a same reaction process.

The invention discloses a goldfish autonomous transposon gene gfTol2, which has a nucleotide sequence shown as SEQ ID No:1. The invention also discloses a transposase coded by the gene gfTol2, which has an amino acid sequence shown as SEQ ID No:2. The invention also discloses use of the gene gfTol2 as a gene medicament or a gene reagent for goldfish; and the rate of graded product of the goldfish is improved and offspring mutation is induced by adjusting the concentration of a transcription product of the gene.

The invention discloses a serum protein marker for early screening and diagnosis of breast cancer, which belongs to the technical field of biomedicine. The serum protein marker for early screening anddiagnosis of the breast cancer is any one or a combination of two or more of the proteins encoded by the CEBPA, RalA, p62, PTCH1, BRCA2, HRAS, FUBP1, GATA3, FGFR3, ALK, HISTIH3B or p53 genes. The invention also discloses a kit and a detection method including the serum protein marker for early screening and diagnosis of the breast cancer. Based on the role played by cancer driver genes in the genesis and development of the tumor, the invention customizes a human protein chip encoded by 138 cancer driver genes, which contains 180 human-derived recombinant proteins. Firstly, an early detectionserum marker of the breast cancer is first screened through the protein chip; then verification is performed by using the ELISA indirect method; and finally, a group of breast cancer serum protein marker that can be used for early screening and diagnosis of the breast cancer is screened out. The area under the ROC curve of the combined diagnosis of breast cancer reaches 0.886; the 95% CI is 0.847to 0.925; and when the specificity is ensured to be 92.2%, the sensitivity is 67.9%, and the consistency rate reaches 80.1%.

The invention belongs to the technical field of aquatic breeding, and particularly relates to a growth-related molecular marker in a C-type scavenger receptor gene (LvSRC) of Litopenaeusvannamei and application thereof to genetic breeding of the Litopenaeusvannamei. A batch of markers related to shrimp growth traits are positioned in the genomic level through genome-wide association analysis, a single nucleotide polymorphism (SNP) marker (LvSRC-211-G / T) positioned in a coding region of the LvSRC gene is further screened through gene annotation and by a candidate gene association analysis method, and the genotype of the marker is significantly correlated to the shrimp growth traits (P is smaller than 10<-6>). The marker is positioned at a 211bp position on the LvSRC gene and belongs to G / Ttype mutation, and the GT genotype of the marker is a distinct growth-dominant genotype. The marker provided by the invention can be used as the shrimp breeding molecular marker for assisting in genetic selection of the shrimp growth traits.

The invention relates to isolation, cloning, functional verification and application of an arabidopsis powdery mildew resistance negative control gene EDR6. The invention further relates to a mutant gene of the EDR6 gene, protein coded by the EDR6 gene, application of the protein coded by the EDR6 gene, as well as application of other autophagy related protein comprising ATG5, ATG7, ATG10 and thelike and serving as powdery mildew resistance negative control factors to disease resistant process.

A gene or protein set includes at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, and possibly 40, 45, 50, 55, 60, 65 genes or proteins, antibodies or hypervariable portion thereof directed against the proteins encoded by these genes.

The invention discloses a serum protein marker for gastric cancer early screening and diagnosis, belonging to the technical field of biomedicine. The serum protein marker is any one of or combinationof more than two of proteins coded by TP53, GNAS, PBRM1, SRSF2, SMARCB1, GNA11, ACVR1B, PIK3CA and COPB1. Meanwhile, a kit and a detection method of the serum protein marker are also provided. According to the serum protein marker, the kit and the detection method provided by the invention, based on function of a cancer driving gene in occurrence and development of tumors, a human protein chips coded by 138 cancer driving gene is customized, totally, 180 humanized recombinant proteins are contained, firstly, an early detection serum marker for gastric cancer is screened out primarily through the protein chip, then, verification is executed through an ELISA indirect experiment, a group of gastric cancer serum markers available for gastric cancer early screening and diagnosis is screened out, and the gastric cancer serum markers are combination of proteins coded by the TP53, the SRSF2, the SMARCB1 and the GNA11 genes, and combined gastric cancer diagnosis area under the RCO curve of thegastric cancer serum markers is as high as 0.950, 95% CI is 0.925-0.976, when specificity is guaranteed to be 90.70%, sensitivity is 86.83%, and consistency rate is as high as 80.3%.

The invention discloses a litchi disease-resistant gene LcLTP as well as an encoding protein and application thereof. A litchi disease resistance related gene LcLTP is separated from litchis, the full-length cDNA of the gene is 342bp, and the nucleotide sequence of the gene is as shown in SEQ ID NO: 1; the protein coded by the gene is a lipid transfer protein with the full length consisting of 113 amino acids, and the amino acid sequence is shown as SEQ ID NO: 2. Transcription pattern analysis and plant expression analysis find that the LcLTP gene can improve the resistance of plants to phytophthora, so that the litchi disease-resistant gene LcLTP can be applied to the aspect of improving the disease resistance of the plants or breeding disease-resistant plants.

The invention discloses a garrupa MHC (Major Histocompatibility Complex) IIB gene as well as a cloning method and application thereof. The nucleotide sequence of the garrupa MHC IIB gene is shown as SEQ ID NO:1, and the amino acid sequence of protein encoded by the gene is shown as SEQ ID NO:2. In the invention, by using a homologous cloning method, the MHC IIB gene is separated from garrupa, 12 different MHC IIB gene types are identified, the correlation between the polymorphism and the disease resistance of the MHC IIB gene are analyzed, and 6 molecular marks of the MHC IIB gene relevant to the disease resistance of the garrupa are screened; and the cloning method is suitable for screening the marks relevant to the disease resistance of the garrupa and breeding disease-resistant species.

The present invention relates to an infectious particle having a surface displaying a ligand binding to a CD4 receptor for selectively infecting dividing CD4+ cells, said particle comprising: (a) one or more structural proteins, and (b) a vector containing a gene of interest functional in a CD4+ cell, said gene of interest encoding a Forkhead box protein 3 or a protein inducing expression of Forkhead box protein 3 in the CD4+ cell.

The invention discloses an associated protein for regulating and controlling round-leaf characteristics of cucumbers as well as a coding gene thereof and application thereof. An amino acid sequence ofthe protein is as shown in SEQ ID NO:2; protein kinase, which belongs to an AGC kinase family, of serine / threonine is coded by a PID gene; a coding gene of the protein is a base sequence as shown ina sequence table SEQ ID NO:1; guanine (G), at a site 1092bp, of the base sequence is substituted by adenine (A), Val (valine) at a site 346 of a coded amino acid residue sequence is mutated to be Met(methionine); the protein inhibits polar translocation of auxin, causes polar defect of auxin transport, has a blade edge smooth plant phenotype, has unapparent saw-tooth bugles, and has nearly roundleaves; a main root is not developed, and a main lateral root does not have obvious difference; male and female flowers are free of calyxes, and female flowers are not developed and the like. The geneand the protein can provide a basis for improving cucumber leaf type, increasing the yield, cultivating ideal plants and researching an auxin signal channel molecule regulation mechanism.

The invention describes HLA class II binding peptides encoded by the SSX-2 tumor associated gene, as well as nucleic acids encoding such peptides and antibodies relating thereto. The peptides stimulate the activity and proliferation of CD4+ T lymphocytes. Methods and products also are provided for diagnosing and treating conditions characterized by expression of the SSX-2 gene.

The invention belongs to the technical field of genes and especially relates to application of a gene Loc_Os01g12810. The gene Loc_Os01g12810 can be applied to rice chloroplast development regulationmechanism researches; a gene method is utilized to regulate an expression level of the gene Loc_Os01g12810 to research rice chloroplast development regulation mechanisms; a nucleotide sequence of thegene Loc_Os01g12810 is shown in SEQ ID NO:1 in a sequence table; an amino acid sequence of encoding protein of the gene Loc_Os01g12810 is shown in SEQ ID NO:2 in the sequence table. According to the application of the gene Loc_Os01g12810 disclosed by the invention, that the rice gene Loc_Os01g12810 is a gene related with rice chloroplast development is proved for the first time. The Loc_Os01g12810is a PPR gene. Cloning and biological function verification of the gene have important reference significance in rice chloroplast development molecule mechanism researches and researches on effects of PPR family gene in rice development.

The present invention relates to methods and compositions of a novel gene and the peptide encoded by the gene. Mutations in the gene, named atlastin, are factors in the disease Hereditary Spastic Paraplegia and related disorders. The present invention will be used for the in the research, diagnosis and treatment of these disabling diseases.

The invention relates to a rice receptor kinase gene OsRLCK21, a protein encoded by the rice receptor kinase gene OsRLCK21 and application. A nucleotide sequence of the rice receptor kinase gene OsRLCK21 is shown as SEQ ID NO.2, and a protein sequence encoded by the gene is shown as SEQ ID NO.1. The OsRLCK21 gene in rice is knocked out through CRISPR / Cpf1 technology in a fixed-point mode to perform a function study of the gene, a result shows that the rice knocked out of the OsRLCK21 gene has significantly improved resistance to Xanthomonas oryzae IV and GD1358, IV lesion length is shortened by 26.4%-48.8%, C5 lesion length is shortened by 43%-52.3%, rice gene mutations can be caused by inserting or deleting a 503 position to a 508 position in a sequence shown SEQ ID NO.2, and a trait of increased resistance to Xanthomonas oryzae is shown.

This invention relates to the androgen-regulated gene, PMEPA1, and proteins encoded by this gene, including variants and analogs thereof. Also provided are other androgen-regulated nucleic acids, a polynucleotide array containing these androgen-regulated nucleic acids, and methods of using the polynucleotide array in the diagnosis and prognosis of prostate cancer.

An object of the present invention is to clone a gene which is involved in synthesis of fatty acid having trans-11-, cis-13-conjugated double bonds from fatty acid having a double bone at position Δ12. The present invention provides a gene having any one of the following nucleotide sequences:(A) a nucleotide sequence encoding an amino acid sequence shown in SEQ ID NO: 1 or 12;(B) a nucleotide sequence encoding an amino acid sequence comprising a deletion, addition or substitution of one or several amino acids with respect to the amino acid sequence shown in SEQ NO: 1 or 12, and having an ability of synthesizing fatty acid having trans-11-, cis-13-conjugated double bonds from fatty acid having a double bond at position Δ12;(C) a nucleotide sequence shown in SEQ ID NO: 2 or 13;(D) a nucleotide sequence comprising a deletion, addition or substitution of one or several nucleotides with respect to the nucleotide sequence shown in SEQ ID NO: 2 or 13, and encoding a protein having an ability of synthesizing fatty acid having trans-11-, cis-13-conjugated double bonds from fatty acid having a double bond at position Δ12; and(E) a nucleotide sequence hybridizing with the nucleotide sequence shown in SEQ ID NO: 2 or 13 or a complementary sequence thereof under stringent conditions, and encoding a protein having an ability of synthesizing fatty acid having trans-11-, cis-13-conjugated double bonds from fatty acid having a double bond at position Δ12.

The present invention belongs to the field of biomedicine preparing technology, and is especially enterohemorrhagic E. coli (EHEC) O157 shiga-like toxin II resisting subunit Stx2A monoclonal antibody 5F3 light and heavy chain variable region gene and the gene coded polypeptide and their application in preparing medicine for diagnosing and treating EHEC O157 infection and its complication. The present inventor adopts common facultative primer to culture one hybridoma cell strain of Stx2A specific monoclonal antibody 5F3 and clones its corresponding light and heavy chain variable region gene. Based on the light and heavy chain variable region gene, several small molecular gene engineering antibodies, such as ScFv antibody, chimeric antibody, antibody fusing protein, etc are constituted and expressed. The present invention provides new way for diagnosing and treating EHEC O157 infection and its complication.

The invention provides a gene, which is a deoxyribonucleic acid (DNA) molecule with the sequence of nucleotide illustrated as the SEQ ID NO:1. A fusion protein of the gene code and a preparation method and application thereof are further provided. The fusion protein mm CTLA4Ig can prevent a CD 28 molecule from being combined with a B7 molecule, further suppresses activation of a T cell, has an obvious immunosuppression function, can be used for preparing an immunosuppressive agent, and is particularly suitable for the immunosuppressive agent for rhesus monkeys.

The invention belongs to the field of molecular biology, and particularly discloses a group of gRNA targeting a mouse Atp7b gene and a method for constructing a Wilson disease mouse model. Three gRNAs specifically targeted to the Atp7b gene are designed, Exon2-Exon20 of the Atp7b gene is knocked out by utilizing cas9 protein, the sequence of a knocked region is not 3 times, frameshift mutation is caused, and the knocked-out region contains 91.91% of a coding region of the gene, so that the aim of knocking out the gene is fulfilled; and the method for constructing the Atp7b gene knockout mouse model by using the CRISPR / Cas9 system is simple and easy to implement, short in period and high in probability of obtaining positive mice, the pathogenesis of the disease can be fully researched by utilizing the mouse model, and service is provided for further developing a treatment mode aiming at the disease.

The invention discloses a method for improving the expression level of human coagulation factor IX. The method comprises the following steps: optimizing human coagulation factor IX gene; replacing Kozak sequence; and constructing AAV (adeno-associated virus) expression plasmid by the optimized human FIX gene and Kozak sequence, and packaging the recombinant AAV. The expression level of the human coagulation factor FIX gene is remarkably improved by the optimized gene coding sequence and Kozak sequence. The optimized human FIX gene and Kozak sequence can be used for efficiently producing FIX protein for treating hemophilia B, and the production cost of the FIX protein is reduced; and the optimized human FIX gene and Kozak sequence can also be used in AAV-mediated gene replacement therapy totreat hemophilia B, the expression level of the FIX gene in human bodies is increased, so that the administration dosage is reduced, and the therapeutic effect is improved.

A light chain variable region gene resisting the CD 20 monoclonal antibody HI47 mutation, the polypeptide coded by said gene, and the application of the fusion protein containing said gene and polypeptide in preparing the medicines for diagnosing and treating tumor are disclosed. The purified expression product of said mutant has the activity to combine with Raji cells expressing CD20 antigen. Its expression product and activity are increased.

Provided is a gene related to astaxanthin accumulation in haematococcus pluvialis and a screening method of the gene. The gene is HpHDR, the sequence length of the gene is 1421bp, and the gene sequence is as shown in SEQ NO.1; HpHDR protein coded through the gene HpHDR is composed of 434 amino acid residues, the molecular formula is C2129H3363N601O656S24, the molecular weight is about 48.64 KD, the theoretical isoelectric point is 6.53, a spatial structure of the protein comprises 15 alpha-helical structures and 12 beta-folded structures, and the amino acid sequence is as shown in SEQ NO.2. The invention provides the screening method of the gene HpHDR, the screening method is high in sensitivity, can detect more samples and is high in screening speed and easy to operate.

Provided are a HNF-1 α gene including a novel single-nucleotide polymorphism and a protein encoded by the HNF-1 α gene, a polynucleotide associated with MODY3 diabetes based on the HNF-1 α gene, a microarray and a diagnostic kit including the polynucleotide, and a method for diagnosis of MODY3 diabetes.