102 results about "EDARADD" patented technology

The invention belongs to the field of plant genetic engineering, and discloses a key gene OsHPCA1 for positively regulating salt stress and oxidative stress tolerance of rice. The gene (with the login number of Os05g40770) encodes a receptor protein capable of specifically sensing hydrogen peroxide, and belongs to a leucine repeat receptor-like protein kinase (LRR-RLKs) family, wherein the family is the largest subfamily in the receptor-like protein kinase (RLKs). The OsHPCA1 responds to salt and oxidative stress signals, and the salt tolerance and oxidative stress resistance of the rice are positively regulated and responded. The OsHPCA1 overexpression rice strain shows very strong salt tolerance; and the OsHPCA1 knockout strain oshpca1 is more sensitive to salt stress. The OsHPCA1 protein encoded by the OsHPCA1 gene and rich in leucine repeat receptor-like protein kinase can positively regulate and control the activity of catalase C (CatC) in rice and enhance the removal efficiency of H2O2 under salt stress, so that the salt tolerance of rice is improved. When the OsHPCA1 is overexpressed, the activity of CatC in the overexpressed strain OsHPCA1 is obviously increased, the H2O2 removal capability is enhanced, and the salt tolerance and oxidative stress tolerance of the rice are improved.

The invention belongs to the technical field of biological medicine, and particularly relates to a regulatory element or a combination thereof. The regulatory element comprises a DNA regulatory element and / or an RNA regulatory element; the DNA regulation element is specifically combined with a protein coded by a target gene so as to inhibit transcription of the target gene; a transcription product of the RNA regulation element is specifically combined with a protein coded by a target gene so as to inhibit translation of the target gene, and when the content of the protein coded by the target gene is high, the expression quantity of the protein can be reduced; when the content of the protein coded by the target gene is low, the expression quantity of the protein can be up-regulated, and the decline level of the protein can be reduced, so that the expression level of the target gene is regulated along with the content of the protein, feedback type self-regulation is formed, and the occurrence of overexpression can be reduced under the condition that a carrier containing the target gene and a regulation element is excessively transfected; therefore, the gene is used for preventing and treating diseases caused by insufficient expression of the target gene, and side effects of overexpression of the target gene are reduced while disease symptoms are improved.

The invention belongs to the technical field of microbial genetic engineering, and particularly relates to an alpha-2, 6-sialyltransferase mutant and application thereof. The a-2, 6-sialyltransferase mutants M133L, E140D, D228H, N289D and G322Q are obtained by carrying out site-specific mutagenesis on five amino acids in an a-2, 6-sialyltransferase amino acid sequence coded by a gene derived from Photobacterium sp.JT-ISH-224, and compared with wild type alpha-2, 6-sialyltransferase, the yield of the single-point mutant 6 '-SL of the E140D and the yield of the single-point mutant 6'-SL of the G322Q are increased by 87.80% and 78.01% respectively. The yield of the optimal recombinant microbial strain 6 '-SL-7 (E140D / G322Q) obtained by mutation site combination can reach 1.471 g / L, the bottleneck problem of low catalytic efficiency of sialyltransferase is effectively solved, and an enzyme element with excellent performance is provided for high-efficiency and low-cost biological manufacturing of 6'-SL.

The invention discloses an NCgl1764 gene mutant and an application of the NCgl1764 gene mutant in preparation of L-lysine. The invention provides an NCgl1764 protein mutant. The NCgl1764 protein mutant comprises any one of the following proteins: a protein obtained by replacing the 64th site of NCgl1764 protein as shown in SEQ ID No.2 with other amino acids from F; or a protein which is derived from bacteria, has more than 95% of identity with the protein and is related to the production of L-amino acid by the bacteria; or a protein which is obtained by substituting and / or deleting and / or adding one or more amino acid residues of the protein and is related to production of L-amino acid by bacteria; or a fusion protein obtained by connecting a label to the N end and / or C end of the protein. The invention proves that the yield of L-lysine can be obviously increased by performing point mutation on the NCgl1764T191C in the coding region of the NCgl1764 gene in corynebacterium glutamicum. The invention has important significance on the yield of L-lysine.

The application belongs to the technical field of biology, and specifically discloses a truncated mutant of ANKRD11 and application thereof. The ANKRD11 gene mutant is any one of the following: a nucleic acid, wherein the nucleic acid has a target fragment, and the target fragment is compared with a wild-type ANKRD11 gene with a sequence of SEQ ID NO. 1, nucleotides from No. 1910 to No. 1913 are deleted; a polypeptide, wherein the polypeptide has a p.K637Tfs*15 mutation compared with a protein encoded by a wild-type ANKRD11 gene with a sequence of SEQ ID NO. 2. The application also relates to application of a reagent for detecting the aforementioned ANKRD11 gene mutant in screening of a KBG syndrome risk population. In the present disclosure, the pathogenic gene spectrum of the KBG syndrome is widened, the understanding of the disease is strengthened, experience is provided for clinical screening and diagnosis of the disease, and a basis is provided for prenatal diagnosis.

The invention relates to a CRISPR-Cpf1-based single plasmid gene editing system and application thereof, and belongs to the technical field of gene editing. According to the invention, a plasmid pcr19NM is used as a carrier, a crRNA sequence insertion region, a gene for coding Cpf1 protein, a gene for coding NgAgo protein and a replicon pE194ts are connected to the pcr19NM, and expression of the Cpf1 protein and the NgAgo protein is regulated and controlled through a promoter Pm1, so that a recombinant plasmid pWLBE-Pm1Cpf1Ng is obtained. The crRNA of a target gene is introduced into a crRNA sequence insertion region, and a homologous arm is connected to a recombinant plasmid pWLBE-Pm1Cpf1Ng, so that gene knockout, base modification or integration can be realized. Based on the single plasmid gene editing system provided by the invention, accurate gene editing can be efficiently completed by transforming a single plasmid, including complete knockout of two genes, base modification of six genes or integration of one gene. According to the single plasmid gene editing system disclosed by the invention, the complexity of operation is reduced, the gene editing period is shortened, and the method for applying the bacillus subtilis to genetic manipulation is expanded.

Described herein are compounds that modulate splicing of a pre-mRNA, encoded by genes, and methods of treating diseases and conditions associated with gene expression or activity of proteins encoded by genes.

This invention belongs to the field of microbial genetic engineering technology, specifically relating to an α-2,6-sialic acid transferase mutant and its application. This invention utilizes a mutant derived from *Lithocarpus lucida* (…). Photobacterium Five amino acids in the amino acid sequence of α-2,6-sialyl transferase encoded by the gene of sp. JT-ISH-224 were mutated at specific sites to obtain α-2,6-sialyl transferase mutants M133L, E140D, D228H, N289D, and G322Q. Among them, the E140D and G322Q single-site mutants of α-2,6-sialyl transferase showed increases in 6'-SL yield of 87.80% and 78.01%, respectively, compared with wild-type α-2,6-sialyl transferase. The optimal recombinant microbial strain 6'-SL-7 (E140D / G322Q) obtained by combining the mutation sites achieved a yield of 1.471 g / L, effectively solving the bottleneck problem of low catalytic efficiency of sialyl transferase and providing a high-performance enzyme element for the efficient and low-cost biomanufacturing of 6'-SL.

The application belongs to the field of plant genetic engineering, and discloses a key gene for positively regulating salt stress and oxidation stress tolerance of rice OsHPCA1 . The gene (accession number: Os05g40770) encodes a receptor protein specifically sensing hydrogen peroxide, which is classified into the leucine repeat receptor-like kinase (LRR-RLKs) family, which is the largest subfamily in the receptor-like kinase (RLKs) family. OsHPCA1 In response to salt and oxidation stress signals, the rice salt and oxidation stress tolerance is positively regulated. OsHPCA1 The overexpression rice strain shows strong salt tolerance; and OsHPCA1 the knockout strain oshpca1 is more sensitive to salt stress. OsHPCA1 The leucine repeat receptor protein kinase OsHPCA1 encoded by the gene can positively regulate the activity of catalase C (CatC) in the rice body, enhance the H2O2 clearance efficiency under salt stress, and thus improve the salt tolerance of the rice. When OsHPCA1 overexpressed, the activity of CatC in the overexpression strain OsHPCA1 is significantly increased, the H2O2 clearance capacity is enhanced, and the salt tolerance and oxidation stress tolerance of the rice are improved.

The invention relates to a CllaF3 '5' H gene and application thereof. The CllaF3 '5' H gene comprises a nucleotide sequence as shown in SEQ ID NO: 1. The CllaF3 '5' H protein comprises a protein coded by the CllaF3 '5' H gene. According to the invention, a CllaF3 '5' H gene is screened and cloned from clematis lanceolata, and the correlation between the CllaF3 '5' H gene and the content of flavonoid metabolites such as delphinidin is found through research; stable germplasm with blue flower potential can be formed by utilizing the gene.

The present invention discloses an AfGF14i gene of Aechmea fasciata, a cloning method, an expression vector and applications thereof, belonging to the field of biotechnology. Specifically, total RNA of Aechmea fasciata is extracted, reverse transcribed into cDNA, and taking this as a template, specific primers are designed, and the full-length CDS of the AfGF14i gene is obtained by PCR method, ligated with a vector, and transformed into Escherichia coli Trans-T1 competent cells, and positive clones are picked to obtain the AfGF14i gene of Aechmea fasciata. The full-length CDS of this gene is 729 bp, encoding a protein containing 243 amino acid residues. The present invention uses transient expression and fluorescence microscopy to detect that the subcellular localization of the protein encoded by the AfGF14i gene is the cell membrane. Through transgenic technology, it is found that the overexpression of the AfGF14i gene promotes the early flowering of Arabidopsis thaliana. The acquisition of the AfGF14i gene lays a foundation for studying the flowering mechanism of Aechmea fasciata, provides a theoretical reference for overcoming the high-temperature obstacle of ethylene-induced flowering of pineapple in production, and has important theoretical and practical significance.

The invention provides a mutant of a YciE gene coding protein and application of the mutant in preparation of NMN, belongs to the technical field of molecular biology, and particularly provides the mutant of the YciE gene coding protein, the amino acid mutation site is that the 140th methionine of the amino acid sequence SEQ ID NO.3 of the YciE gene coding protein is mutated into isoleucine; the coding gene of the mutant is as shown in SEQ ID NO. 2; a recombinant bacterium containing the coding gene of the mutant; according to the invention, it is found for the first time that methionine at the 140th site in a protein amino acid sequence coded by a YciE gene of escherichia coli is mutated into isoleucine; the synthesis level of NMN can be effectively improved, and generation of by-products is reduced.

The application discloses an immunoregulatory peptide BugaCATH and application thereof, and belongs to the field of biomedicine. Buga The CATH is a cyclic polypeptide with a pair of intramolecular disulfide bond composed of a sixth cysteine and a thirteenth cysteine, and has a molecular weight of 3156.67 Dalton and an isoelectric point of 8.76, wherein the amino acid sequence is shown as SEQ ID NO: 2. Buga The gene (GenBank accession: OQ870533) of the CATH precursor is composed of 663 nucleotide sequences, and the nucleotide sequence is shown as SEQ ID NO: 1; wherein the nucleotide at the positions of 322-448 is the immunoregulatory peptide Buga The encoding gene of the CATH. The immunoregulatory peptide Buga The application of the CATH in the preparation of a treatment drug for promoting skin wound repair.

The application discloses a Phyllostachys edulis-derived PeDHN2 gene and application of a coded protein thereof, and belongs to the technical field of plant biological breeding. A nucleotide sequence of the PeDHN2 gene is shown as SEQ ID NO. 11; and a sequence of the coded protein of the PeDHN2 gene is shown as SEQ ID NO. 12. The PeDHN2 gene is cloned from the Phyllostachys edulis, and experiments show that the gene is significantly up-regulated under salt stress, drought and abscisic acid (ABA) stress, and after being introduced into rice through a transgenic technology, the germination rate and root growth ability of the rice under salt stress are significantly improved. The application provides a key gene resource and technical support for molecular design breeding of salt-tolerant crops.

The application relates to the technical field of molecular markers, and discloses an SNP site associated with turbot albinism, a molecular marker and application, wherein the SNP mutation site is a base T / A mutation at the 50th bp of a coding region of a gene KIT proto-oncogene, receptor tyrosine kinase a (kita); the SNP mutation site is a base A / T mutation at the 137th bp of a coding region of a gene cytochrome P450 3A40-like (LOC118311907); the SNP mutation site is a base G / T mutation at the 371st bp of a coding region of a gene kit ligand a (kitlga); and the SNP mutation site is a base G / A mutation at the 581st bp of a coding region of a gene frizzled class receptor 10 (fzd10). The SNP site associated with turbot albinism, the molecular marker and the application provide a precise screening method for turbot breeding. In the breeding process, the SNP molecular markers are used to determine whether the genotype is albinism or normal, to establish an excellent family with normal body color, to reduce the incidence of abnormal body color, and to improve the overall quality and economic benefits of turbot culture.

This invention belongs to the field of biotechnology, specifically disclosing novel mutations of ANKRD11 and their applications. The ANKRD11 gene mutation can be any of the following: a nucleic acid having a target fragment, wherein the target fragment has a G repeat at position 4708 compared to the wild-type ANKRD11 gene with sequence SEQ ID NO.1; or a polypeptide having the p.E1570Gfs*71 mutation compared to the wild-type protein encoded by the ANKRD11 gene with sequence SEQ ID NO.2. The invention also relates to the application of reagents for detecting the aforementioned ANKRD11 gene mutation in screening individuals at risk for KBG syndrome. This disclosure broadens the pathogenic gene spectrum of KBG syndrome, enhances the understanding of the disease, provides experience for clinical screening and diagnosis of the disease, and also provides a basis for prenatal diagnosis.

Described herein are compounds that modulate splicing of a pre-mRNA, encoded by genes, and methods of treating diseases and conditions associated with gene expression or activity of proteins encoded by genes.

The present invention relates to: a composition for diagnosing colorectal cancer, comprising a substance capable of measuring the relative expression levels of CCR1, CD177, DLG5, EPCAM, ERBB2, FCGR1A, FOXA2, GK, KRT19, KRT73, MKI67, MPO, MUC1, NME1, NPTN, NQO2, SH2D1B, SNAI2, TERT, TUG1, and VIM genes or proteins encoded by the genes; a kit for diagnosing colorectal cancer, comprising the composition; and a method for diagnosing colorectal cancer by using the kit.

Described herein are compounds that modulate splicing of a pre-mRNA, encoded by genes, and methods of treating diseases and conditions associated with gene expression or activity of proteins encoded by genes.

The invention relates to pegRNA for specifically targeting pig PKD2 gene encoding protein 658-site leucine, a pilot editing system and application, and belongs to the technical field of molecular biology and the technical field of gene editing. Wherein the pegRNA comprises an sgRNA Spacer, an RT (Reverse Transcription) template and PBS (Phosphate Buffer Solution); wherein the sequence of the sgRNA Spacer is as shown in SEQ ID NO. 6, the sequence of the RT template is as shown in SEQ ID NO. 7, and the sequence of the PBS is as shown in SEQ ID NO. 8. According to the system, leucine at the 658 site of a PKD2 gene encoding protein is mutated into tryptophan (c.1973 Tgt; G, p.L658W), so that ADPKD is induced, and technical support is provided for follow-up research on pathogenesis of the disease and drug screening of verified treatment targets.

The invention belongs to the technical field of gene engineering, biomedicine and molecular diagnosis, and discloses a hearing disorder related HSD17B7 gene mutant and application thereof, the HSD17B7 gene mutant is an autosomal dominant mutant, and the mutation site of the HSD17B7 gene mutant is that the 544th nucleotide G of an HSD17B7 gene coding region on a human 10th chromosome is mutated into T (c.544Ggt; and the 182th amino acid in the protein sequence is mutated from glutamic acid E to a termination codon (p.E182 *). Through exon group sequencing analysis and animal experiment verification on members of the deafness disease family, the invention determines that the mutation has obvious correlation with deafness for the first time, reveals the key effect of the mutation as deafness causing mutation, and constructs a diagnostic kit capable of early identifying the mutation according to the key effect. The method can be widely applied to screening and molecular diagnosis of deafness high-risk groups, and has important scientific research value and clinical application prospect.

The invention discloses an NCgl0762 gene mutant and an application of the NCgl0762 gene mutant in preparation of L-lysine. The invention provides an NCgl0762 protein mutant. The NCgl0762 protein mutant comprises any one of the following proteins: a protein obtained by replacing L at the 21st site of an NCgl0762 protein as shown in SEQ ID No.2 with other amino acids; or a protein which is derived from bacteria, has more than 95% of identity with the protein and is related to the production of L-amino acid by the bacteria; or a protein which is obtained by substituting and / or deleting and / or adding one or more amino acid residues of the protein and is related to production of L-amino acid by bacteria; or a fusion protein obtained by connecting a label to the N end and / or C end of the protein. The invention proves that NCgl0762C61A is subjected to point mutation in the coding region of the NCgl0762 gene in corynebacterium glutamicum, so that the yield of L-lysine can be obviously increased. The invention has important significance on the yield of L-lysine.

The application belongs to the field of plant genetic engineering, and particularly relates to application of a receptor kinase OsCRK17 gene in regulating salt tolerance of rice. An OsCRK17 gene capable of regulating salt tolerance of rice is screened. The nucleotide sequence of the gene is shown as SEQ ID NO:1; the protein sequence encoded by the gene is shown as SEQ ID NO:2. By using Agrobacterium-mediated transformation, a pU1301-OsCRK17 overexpression strain and a CRISPR / Cas9-OsCRK17 gene editing strain are obtained. It is found by salt stress identification of the transgenic materials that the salt tolerance of the OsCRK17 gene overexpression strain is enhanced, indicating that OsCRK17 is a positive regulation factor for regulating salt tolerance of rice, plays a positive regulation role in response of rice to salt stress, and overexpression of the OsCRK17 gene can significantly improve the salt tolerance of rice.

Described herein are compounds that modulate splicing of a pre-mRNA, encoded by genes, and methods of treating diseases and conditions associated with gene expression or activity of proteins encoded by genes.

The invention relates to the field of gene engineering, and discloses a CRISPRi (clustered regularly interspaced short palindromic repeats i) system, a recombinant strain containing the CRISPRi system, a construction method and application of the recombinant strain, and a method for producing gibberellin. The CRISPRi system comprises a coding gene of a dCas9 mutant protein without cleavage activity, sgRNA and an induction regulation component, wherein the induction regulation component is used for regulating the expression of the dCas9 mutant protein coding gene; the sgRNA guides the dCas9 mutant protein to be combined to a target gene, so that the expression of the target gene is inhibited; the dCas9 mutant protein is obtained by mutation on the basis of a Cas9 protein, and mutation sites are D10A and H840A of the dCas9 protein; the target genes comprise squalene synthase encoding genes, fatty acid synthase encoding genes and the like, the CRISPRi system can accurately and efficiently regulate and control gibberellin gene expression, the gibberellin yield is remarkably increased, the influence on strain growth is small, regulation and control are reversible, specificity is high, and the CRISPRi system is matched with gibberellin and has good industrial prospects.

Described herein are compounds that modulate splicing of a pre-mRNA, encoded by genes, and methods of treating diseases and conditions associated with gene expression or activity of proteins encoded by genes.

The invention belongs to the field of gene engineering, and relates to research on drought resistance of cotton, in particular to application and a method of a GhDRP4 gene or an encoding protein thereof in improving the drought resistance of the cotton. According to the invention, differential expression genes of drought-tolerant (ZY007) and sensitive (ZY168) cotton under drought stress are analyzed through RNA-seq, and GhDRP4 (SEQ ID No.1) is found to be significantly down-regulated under the drought condition. Functional verification of the GhDRP4 gene (SEQ ID No.1) screened based on transcriptome data shows that the gene encodes U-box type E3 ubiquitin ligase and negatively regulates the drought resistance of cotton. GhDRP4 or CRISPR / Cas9 is silenced through a VIGS technology to knock out the gene (ghdrp4 mutant), so that the drought resistance and survival rate of the cotton are remarkably improved, and a new target is provided for drought-resistant breeding.

The present invention discloses a Bacillus subtilis autoinducing gene expression system based on a two-component quorum sensing element, belonging to the field of genetic engineering technology. A long-lasting and high-expression autoinducing gene expression system is developed. The present invention provides an autoinducing gene expression system, wherein the expression element comprises a constitutive promoter and a target promoter, the RBS sequence of the agrA gene and / or the RBS sequence of the agrD gene, the gene agrA encoding a response regulator protein, the gene agrD encoding the precursor of the signal molecule AIP, the gene agrB encoding a signal processing protein, and the gene agrC encoding a sensor protein that binds to the signal molecule and initiates signal transduction. The present invention provides a new approach and method for designing a recombinant protein expression system based on Bacillus subtilis.

The present application relates to the field of genetic engineering, in particular to a sgRNA group targeting DYA gene, a primer pair for amplifying target sequence, a plasmid group and application thereof. The sgRNA group designed by the present application edits the sheep DYA gene, and after gene editing, large fragment deletion occurs and causes frame shift mutation, which causes great damage to the protein structure and function encoded by the DYA gene. The experimental results show that different fragment lengths of gene editing can be caused in the target region, the overall editing efficiency of the first exon reaches 76.4%, and the overall editing efficiency of the second exon reaches 84.09%. Editing occurs on both sets of chromosomes in the genome (i.e. homozygote): the homozygote editing efficiency of the first exon is 30.9%, and the homozygote editing efficiency of the second exon is 9.1%. The sgRNA group provided by the present application has important significance in the study of ruminant evolution and related immune mechanisms.

The invention belongs to the technical field of enzyme engineering, and relates to degradation of aflatoxin B1 and zearalenone, in particular to a coding gene of an aflatoxin B1 and zearalenone degrading enzyme and application of the coding gene. The coding gene has a similarity of 90% or more with a nucleotide sequence shown as SEQ ID No.2. The coding gene has a nucleotide sequence shown as SEQ ID No.2. The amino acid coded by the gene has 70% or more similarity with the amino acid sequence shown in SEQ ID No.1. The aflatoxin B1 and zearalenone degrading enzyme disclosed by the invention is used for degrading AFB1 and ZEN, the optimum temperature for degrading the AFB1 is 40 DEG C, and the pH is 7.0; the optimal temperature for ZEN is 60 DEG C, and the pH value is 8.0; the degradation rate of the enzyme to AFB1 is 98.82%, the time is prolonged to 36 h, and the highest degradation rate is 99.87%; the degradation rate of ZEN is 96.1%, the time is prolonged to 36 h, and the highest degradation rate is 99.33%.