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Serum protein marker, kit and detection method for gastric cancer early screening and diagnosis

A serum protein and detection method technology, applied in the field of biomedicine, can solve the problems of lack of differential ability in tumor diagnosis, low sensitivity and specificity of monoclonal antibody TAA antibody, etc., and achieve the effect of low cost, high sensitivity and simple operation

Active Publication Date: 2020-01-21
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In general, due to the heterogeneity of tumors, the sensitivity and specificity of monoclonal antibody TAA autoantibodies are low, and the diagnosis of specific tumors lacks discrimination ability

Method used

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  • Serum protein marker, kit and detection method for gastric cancer early screening and diagnosis
  • Serum protein marker, kit and detection method for gastric cancer early screening and diagnosis
  • Serum protein marker, kit and detection method for gastric cancer early screening and diagnosis

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Experimental program
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Effect test

experiment example

[0038] 1 Preparation of serum samples

[0039] 1.1 Serum samples for protein chip experiments

[0040]Patients with primary gastric cancer (gastric cancer diagnosed by pathology) were collected at Beijing You'an Hospital and the First Affiliated Hospital of Zhengzhou University, with patient consent and approval by the Institutional Review Board and the Hospital Ethics Committee. All samples were collected with red-headed blood collection tubes to collect 5-10mL of whole blood from the research subjects. After standing at room temperature for 2 hours, centrifuge at 1000g for 15 minutes, take the supernatant, divide each sample into several parts and label them, and store them in a low-temperature refrigerator at -80°C. Save and avoid repeated freezing and thawing.

[0041] According to the epidemiological analysis, the sera of 100 cases of primary gastric cancer and 50 cases of normal control sera from You'an Hospital in the same period of physical examination were finally co...

Embodiment

[0106] A serum protein marker for early screening and diagnosis of gastric cancer, the serum protein marker is a combination of proteins encoded by TP53, SRSF2, SMARCB1 and GNA11 genes. The protein encoded by the TP53 gene has the amino acid sequence shown in SEQ ID NO.1; the protein encoded by the SRSF2 gene has the amino acid sequence shown in SEQ ID NO.3; the protein encoded by the GNA11 gene has the amino acid sequence shown in SEQ ID NO. The amino acid sequence shown in NO.5; the protein encoded by the SMARCB1 gene has the amino acid sequence shown in SEQ ID NO.10.

[0107] A kit comprising the aforementioned serum protein markers for early screening and diagnosis of gastric cancer, the serum protein markers are coated on a solid-phase carrier, and the solid-phase carrier is made of polyvinyl chloride Concave plate. The kit also includes positive control serum, negative control serum, blocking solution, serum diluent, secondary antibody, secondary antibody diluent, washi...

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Abstract

The invention discloses a serum protein marker for gastric cancer early screening and diagnosis, belonging to the technical field of biomedicine. The serum protein marker is any one of or combinationof more than two of proteins coded by TP53, GNAS, PBRM1, SRSF2, SMARCB1, GNA11, ACVR1B, PIK3CA and COPB1. Meanwhile, a kit and a detection method of the serum protein marker are also provided. According to the serum protein marker, the kit and the detection method provided by the invention, based on function of a cancer driving gene in occurrence and development of tumors, a human protein chips coded by 138 cancer driving gene is customized, totally, 180 humanized recombinant proteins are contained, firstly, an early detection serum marker for gastric cancer is screened out primarily through the protein chip, then, verification is executed through an ELISA indirect experiment, a group of gastric cancer serum markers available for gastric cancer early screening and diagnosis is screened out, and the gastric cancer serum markers are combination of proteins coded by the TP53, the SRSF2, the SMARCB1 and the GNA11 genes, and combined gastric cancer diagnosis area under the RCO curve of thegastric cancer serum markers is as high as 0.950, 95% CI is 0.925-0.976, when specificity is guaranteed to be 90.70%, sensitivity is 86.83%, and consistency rate is as high as 80.3%.

Description

technical field [0001] The invention belongs to the technical field of biomedicine. Background technique [0002] Gastric cancer is a common and highly lethal malignant tumor, and its mortality rate ranks second among cancers. The early diagnosis rate and 5-year survival rate of advanced gastric cancer are both <20%. Currently, the detection methods widely used in gastric cancer include non-invasive examinations (such as B-ultrasound, CT, etc.) and invasive examinations (gastroscopy, barium meal, etc.), but there is a lack of compliance and the possibility of popularization. Therefore, new biomarkers are needed to detect gastric cancer in time, especially serum molecular markers, so that gastric cancer patients can be detected, diagnosed and treated in a timely and effective manner, which is the key to improving the survival rate and reducing mortality of gastric cancer patients. Although some tumor markers, such as CA125 (cancer antigen 125), CA19-9 (cancer antigen 19-9...

Claims

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Application Information

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IPC IPC(8): G01N33/536G01N33/574G01N21/31
CPCG01N33/536G01N33/57446G01N33/57484G01N21/31
Inventor 张建营王鹏王晓叶华史健翔代丽萍杨倩秦洁洁王科妍江秉华
Owner ZHENGZHOU UNIV
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