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Application of total glycosides of fritillariae cirrhosae bulbus in preparation of asthma treating drug

A technology of Fritillaria glycosides and total glycosides, which is applied in the field of application of total glycosides of Fritillaria chinensis in the preparation of drugs for the treatment of asthma, and achieves the effects of relieving asthma, considerable development and utilization value, and decreasing the proportion of eosinophils

Inactive Publication Date: 2020-01-24
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Ovalbumin (OVA) is the most classic asthma model sensitizer, and the OVA mouse asthma model is also the most mature animal model so far. There is no report on the relief and treatment of Chuanbeimu total glycosides

Method used

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  • Application of total glycosides of fritillariae cirrhosae bulbus in preparation of asthma treating drug
  • Application of total glycosides of fritillariae cirrhosae bulbus in preparation of asthma treating drug
  • Application of total glycosides of fritillariae cirrhosae bulbus in preparation of asthma treating drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Optimization of extraction conditions for the enrichment and extraction of total glycosides of Fritillaria

[0030] 1. Macroporous resin type screening

[0031] The present invention compares the static adsorption and desorption properties of four types of macroporous resins, AB-8, D101 and HPD100, so as to screen the macroporous resin suitable for the enrichment of fritillary glycosides. Take 4ml of the loading solution (total glycoside concentration 0.47mg / ml), add it to the Erlenmeyer flask containing 1.0g of macroporous resin, prepare 3 copies of each type of resin in parallel, weigh it, seal it, and place it on a shaker for full shaking 12h, so that the macroporous resin is fully absorbed. After 12 hours, weigh and make up the weight, pipette the supernatant, centrifuge at 13000rpm for 5min, prepare and detect by vanillin-glacial acetic acid-perchloric acid colorimetric method, and calculate the ratio of various resins to total glycosides of Chuanbeimu ...

example 2

[0036] Example 2: Separation and preparation of fritillary glycosides of the present invention

[0037] The preparation method of Chuanbeimu total glycosides extract (such as image 3 shown):

[0038] Take Fritillaria bulb powder (through a 60-mesh sieve), extract with 70% ethanol (85°C, slightly boil), the ratio of solid to liquid is 1:10, extract for 3 hours, extract twice, filter, combine the filtrates, and concentrate the filtrates into the original 1 / 3 of the volume (steamed until there is no alcohol smell), to prepare the total glycosides of Chuanbeimu. Degrease with petroleum ether 3 times (extraction volume 1:1) to obtain the aqueous phase layer, extract 5-6 times with water-saturated n-butanol (extraction volume 1:1), evaporate the solvent and concentrate to obtain the extract, redissolve in water, freeze Dry to get the extract. The extract was dissolved in single distilled water (dissolved according to the ratio of 1.0 mg raw medicinal material: 1 ml water), and s...

example 3

[0039] Example 3: Preparation of Chuanbeimu total glycosides preparation

[0040] Dissolve 20 mg of fritillary glycosides extract in 10 ml of 0.5% CMC-Na, sonicate for 20 min, vortex to a suspension state, and make an oral solution.

[0041] 20 mg of the total glycosides extract of Fritillaria chinensis is mixed with 50 mg of dextrin and 20 mg of powdered sugar, and 30% ethanol is used as a wetting agent to make a soft material to prepare granules.

[0042] 20 mg of the total glycosides extract of Chuanbeimu, 20 mg of powdered sugar and 5 mg of water are prepared into a syrup by conventional technology, and then 70 mg of starch is added to prepare a pill by conventional technology.

[0043] 20 mg of total glycosides extract of Fritillaria chinensis is mixed with 50 mg of starch and 50 mg of dextrin, and an appropriate amount of 30% ethanol is used as a wetting agent to make a soft material, granulated by a conventional method, and mixed with magnesium stearate to make a tablet. ...

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Abstract

The invention discloses application of total glycosides of fritillariae cirrhosae bulbus in preparation of anasthma treating drug and solves the problems of single way, large side effects and production of drug resistance caused by other single target medication. The total glycoside extract of fritillariae cirrhosae bulbus can provide safer and more effective treatment, has multi-effect and multi-target action mechanisms, can effectively treat asthma according to clinical pathological characteristics, provides a new direction for seeking an anti-asthma drug and has good clinical application prospects.

Description

technical field [0001] The invention relates to the application of natural medicines, in particular to the application of total glycosides of Chuanbeimu in the preparation of medicines for treating asthma. Background technique [0002] Asthma (Asthma) is a recurrent chronic airway inflammatory disease caused by the infiltration of various inflammatory cells, such as eosinophils, mast cells and T lymphocytes, and is clinically characterized by airway hyperresponse , prominently manifested as reversible airway obstruction and dyspnea, the disease is highly heterogeneous, and its causes are wide, such as dust mites, pollen or air pollution, etc., which can all be regarded as the cause of asthma. [0003] Despite wide heterogeneity in the development of allergic responses, Ig-E plays a crucial role in bridging innate and specific immunity in allergic asthma and is involved in all stages of asthma. In the early stage of asthma, antigens are presented to CD4+ T cells by dendritic...

Claims

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Application Information

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IPC IPC(8): A61K36/8966A61P11/06A61P29/00
CPCA61K36/8966A61P11/06A61P29/00A61K2236/333A61K2236/51A61K2236/55
Inventor 辛贵忠杨丽刘丽芳刘云
Owner CHINA PHARM UNIV
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