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A control fingerprint of virgin olive oil and its construction method and application

A technology for comparing fingerprints and virgin olive oil, applied in the field of detection, can solve the problems of misjudgment, adulteration types and large ranges of adulteration, etc.

Active Publication Date: 2022-03-11
LONGNAN XIANGYU OIL OLIVES DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, some studies have shown that the fatty acid composition of some vegetable oils is relatively similar, so there is a certain risk in identifying and identifying these oils with similar fatty acid composition through fatty acid fingerprints, and the types and amounts of adulteration are also relatively large, so only Possibility of Misjudgment Using Fatty Acid Fingerprints to Identify Adulterated Oils

Method used

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  • A control fingerprint of virgin olive oil and its construction method and application
  • A control fingerprint of virgin olive oil and its construction method and application
  • A control fingerprint of virgin olive oil and its construction method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0091] 1.1 Establishment of virgin olive oil control fingerprint

[0092] 1.1.1. Take 10.0 g of 17 batches of extra virgin olive oil, weigh accurately, put it in a 50 mL polyethylene tube, add 20 mL of methanol precisely, vortex for 1.0 min, and centrifuge at 10,000 r / min, then take 1.5 mL of the supernatant. , filtered with a 0.22 μm organic phase microporous membrane to obtain 17 extra virgin olive oil samples.

[0093] 1.1.2. Perform ultra-high performance liquid chromatography-fluorescence detection on 17 extra virgin olive oil samples to obtain chromatographic information of 17 extra virgin olive oil samples. figure 1 It is an overlay of the chromatographic information of 9 extra virgin olive oil samples among the 17 extra virgin olive oil samples;

[0094] 1.1.3. Calculate the similarity of the chromatographic information of the 17 extra virgin olive oil samples, obtain the virgin olive oil control fingerprint, and set the chromatographic peaks whose peak area is greate...

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Abstract

The invention provides a comparison fingerprint of virgin olive oil and its construction method and application, belonging to the technical field of detection. The invention adopts ultra-high performance liquid chromatography-fluorescence detection to analyze a series of extra virgin olive oils, and obtains the chromatographic information of various components in the extra virgin olive oils, which breaks through the traditional GC-MS method, based on the analysis of edible oil fatty acids The limitations of constructing fingerprints based on the components also solve the problem that traditional fingerprints only use a single component for discrimination and cause false positives. At the same time, the similarity calculation is carried out on the chromatographic information of a series of extra virgin olive oil, and the comparison fingerprint of extra virgin olive oil is established, which strengthens the polymorphism of the fingerprint information, and reflects the relationship between the composition, distribution and content of extra virgin olive oil from multiple angles. The difference between edible oils is conducive to improving the accuracy of identification, and can easily, quickly and accurately identify the quality of virgin olive oil.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a virgin olive oil control fingerprint and a construction method and application thereof. Background technique [0002] Olive oil refers to the oil prepared from the fresh olive fruit (excluding the oil obtained by solvent extraction or heavy esterification), and is not mixed with other types of oil. Virgin olive oil has the highest quality and nutritional value. It is rich in unsaturated fatty acids, squalene, polyphenols, vitamins and other natural active substances. It has anti-oxidation, cholesterol regulation, cancer prevention, beauty and other effects. The price is higher than other edibles. Vegetable oil is much higher. Because olive oil is favored by people because of its unique physical and chemical properties and excellent nutritional and health care effects, in view of this, some businessmen are driven by economic interests, and the problem of olive oil adulteratio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/34G01N30/36G01N30/74G01N30/86G01N21/64
CPCG01N30/02G01N30/34G01N30/36G01N30/74G01N21/64G01N30/8686G01N2030/027G01N33/03G01N2030/8813G01N1/38
Inventor 王波刘玉红李建科金凤周小平杨永青吴龚鹏赵敏王慧珺张欢
Owner LONGNAN XIANGYU OIL OLIVES DEV
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