Low-cost electrotransfection instrument capacitor charging and discharging circuit and device and cell electrotransfection instrument
A technology of capacitor charging and discharging and charging circuit, which is applied in the field of low-cost electroporator capacitor charging and discharging circuit and cell electroporator, can solve problems such as waste of resources, and achieve the effects of avoiding waste of resources, reducing pressure requirements and reducing production costs.
Inactive Publication Date: 2020-02-04
田志刚
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Problems solved by technology
[0004] However, only using high-voltage IGBTs does not fully guarantee that the cell electroporator is suitable for all cells. Similarly, when dealing with some cells that require little electric field strength, using a cell electroporator with a high-voltage IGBT will also cause unnecessary waste of resources
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[0029] see figure 1 , figure 1 It is a schematic diagram of the structural connection relationship of a low-cost electrotransfer device capacitor charging and discharging circuit provided by an embodiment of the present application.
[0030] see figure 2 , figure 2 It is a schematic circuit structure diagram of a low-cost electrotransfer device capacitor charging and discharging circuit provided by an embodiment of the present application.
[0031] Such as figure 1 and figure 2 As shown, this embodiment provides a low-cost electrotransfer capacitor charging and discharging circuit, which is applied to a low-cost electrotransfer capacitor charging and discharging device. The low-cost electrotransfer capacitor charging and discharging circuit 1 includes a charging circuit 2 and a discharging circuit 3; wherein,
[0032] The charging circuit 2 includes a first relay A1, a first terminal A2, and at least two first switches A3; the first end of the first relay A1 is connect...
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The invention relates to a low-cost electrotransfection instrument capacitor charging and discharging circuit and device and a cell electrotransfection instrument. The low-cost electrotransfection instrument capacitor charging and discharging circuit comprises a charging circuit and a discharging circuit, wherein the charging circuit comprises a first relay, a first terminal and at least two firstswitches; the discharging circuit comprises a second relay, a second terminal and at least two second switches; when the first relay and the first switches are on, and the second relay and the secondswitches are not on, the low-cost electrotransfection instrument capacitor charging and discharging circuit charges a capacitor; and when the first relay and the first switches are not on and the second relay and the second switches are on, low-cost electrotransfection instrument capacitor charging and discharging circuit discharges the capacitor. A method of the switches in series is adopted toperform voltage dividing on charging and discharging voltage, the pressure bearing requirements on the required switches is required, the restriction on the switches due to high voltage requirements is got rid, waste of resources is eliminated, and production cost is reduced.
Description
technical field [0001] The application relates to the technical field of biomedical instruments, in particular to a low-cost electrotransfer instrument capacitor charging and discharging circuit, device and cell electrotransfer instrument. Background technique [0002] Cell electroporation (electrotransfection), also called cell electroporation, is to temporarily form small holes or openings on the cell membrane after an electric field acts on the cell for a few microseconds to a few milliseconds, and macromolecules such as DNA, RNA, siRNA, protein, etc. As well as the technology of introducing some small molecules into cells and finally entering the nucleus, the cells electroporated are different, and the requirements for electric field strength are also different. [0003] In related technologies, the most commonly used instrument for implementing cell electroporation technology is a cell electroporation instrument. The cell electrotransfer instrument completes the cell e...
Claims
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IPC IPC(8): H02J7/00C12M1/42
CPCC12M1/42C12M35/02H02J7/0068H02J7/345
Inventor 田志刚
Owner 田志刚