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A kind of preparation method of red maple artificial seeds

A technology of artificial seeds and production methods, applied in horticultural methods, botanical equipment and methods, planting substrates, etc., can solve problems such as unfavorable management, uneven emergence, large variability, etc., and achieve novel resources, simple production, and stability Good results

Active Publication Date: 2022-08-05
DALIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The biggest feature of Chinese red maple is that the color changes from normal green to red in autumn, "October frost leaves are red like fire", "frost leaves are red like February flowers", but most of China red maple are sown seedlings, and the variability is relatively large. Large, uneven emergence, large differences in growth of offspring, not conducive to management, and affect its ornamental and medicinal value

Method used

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  • A kind of preparation method of red maple artificial seeds
  • A kind of preparation method of red maple artificial seeds
  • A kind of preparation method of red maple artificial seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Screening of artificial endosperm

[0017] Taking MS, 1 / 2MS, 1 / 4MS, and 1 / 8MS as the basic nutrients of artificial endosperm, adding IAA, ZT, GA and maltose as auxiliary nutrients to promote the germination and growth of somatic embryos. The concentration of IAA is set as: 0.5-4.0 mg / L; the concentration of ZT is: 0.1-2.0 mg / L; the concentration of GA is: 0.5-2.0 mg / L; the concentration of maltose is: 1.0-7.0%. An orthogonal design with 5 factors and 4 levels was used to screen the artificial endosperm formula by orthogonal test without considering the interaction. The screening index was based on the rate of somatic embryonic rooting (see Table 1-1).

[0018] Experimental design: In artificial endosperm, the basic medium is MS(A1), 1 / 2MS(A2), 1 / 4MS(A3), 1 / 8MS(A4); IAA concentration is designed as 0.5mg / L(B1), 1.0 mg / L(B2), 2.0mg / L(B3) and 4.0mg / L(B4); ZT concentrations are designed to be 0.1mg / L(C1), 0.5mg / L(C2), 1.0mg / L(C3) and 2.0mg / L (C4); GA concentrat...

Embodiment 2

[0043] Example 2 Screening of artificial seed coats

[0044] Taking Chinese red maple somatic embryos as propagules, 1 / 4MS+IAA2.0mg / L+ZT0.1mg / L+GA1.0mg / L+maltose 7% as artificial endosperm, suitable artificial seed coats were screened. The artificial seed coat is made of sodium alginate with a concentration of 1% to 5% and CaCl with a concentration of 1% to 3%. 2 , The cellulose with a concentration of 1% to 5% and the hemicellulose solution with a concentration of 1% to 5% are matched with each other. A 4-factor and 3-level orthogonal design was used to screen artificial seed coats without considering the interaction. The germination rate of artificial seeds on 1 / 4MS+7% maltose medium was used as the screening index (see Table 2-1).

[0045] Experimental design: In artificial seed coat, the concentration of sodium alginate is 1% (A1), 3% (A2), 5% (A3); CaCl 2 Concentrations are 1% (B1), 2% (B2), 3% (B3); cellulose concentrations are 1% (C1), 3% (C2), 5% (C3); hemicellulose...

Embodiment 3

[0063] Example 3 Screening of transplanted substrates

[0064] Three kinds of substrates, including wood chips, humus and perlite, are used in different proportions (1-5:1-5:1-5). Carry out transplanting and hardening, keep the substrate moist, the air humidity is 70% to 90%, spray water 4 to 5 times a day, and carry out indoor hardening and transplanting.

[0065] Experimental design: In the transplanting matrix, three ratios of chipped wood chips, humus soil and perlite were used, namely chipped wood chips: humus soil: perlite = 5:1:1, chipped wood chips: humus soil : perlite = 1:4:1 and sawdust:humus:perlite = 1:1:5. When examining the above three proportions, the survival rate of artificial seeds (see Table 3-1). 10 seeds per pot were transplanted for each treatment and 5 replicates were set up.

[0066] Table 3-1 Transplanting survival rate (%) on substrates with different ratios

[0067]

[0068] The variance analysis of Table 3-2 to Table 3-1

[0069] ...

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Abstract

The invention discloses a method for making artificial seeds of red maple, belonging to the technical field of artificial seeds. The production method involved in the present invention firstly puts the sterile Chinese red maple somatic embryos into the artificial endosperm on the ultra-clean workbench, mixes them evenly, and then sucks one propagule at a time with a straw and drops it into the artificial seed coat After ion exchange reaction for 15min, rinse with sterile water for 20min, terminate the reaction, pick up and dry to get red maple artificial seeds, and finally, use the transplanting substrate to transplant and refine the seedlings, keep the substrate moist, and the air humidity is 70% to 90% , spray water 4 to 5 times a day, and carry out indoor hardening and transplanting. The red maple artificial seed system prepared by the invention has good stability, is relatively simple to manufacture, and has a high seedling emergence rate. At the same time, Chinese red maple somatic embryos are used as propagules, and the resources used are novel.

Description

technical field [0001] The invention belongs to the technical field of artificial seeds, and particularly relates to a preparation method of red maple seeds. Background technique [0002] Plant artificial seeds (Artificialseeds) or synthetic seeds (Syntheticseeds) are artificial seeds. Artificial seed technology is a new technology developed on the basis of plant in vitro culture technology. Artificial seeds are artificial capsules containing propagules such as plant embryoid bodies or buds, nutrients, hormones and other components. The concept of artificial seeds was first proposed by Murashige at the 4th International Congress of Plant Tissue and Cell Culture in 1978. Murashige believes that with the continuous development of plant tissue culture technology, a large number of embryoid bodies can be simultaneously cultivated with a small number of explants. These embryoid bodies are embedded in a certain capsule to make them function as seeds, which can be directly used ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G31/00A01G24/23A01G24/28A01G24/15
CPCA01H4/006A01H4/001A01G31/00A01G24/23A01G24/28A01G24/15
Inventor 侯义龙蔡军
Owner DALIAN UNIV
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