Porous polymer ultrasound contrast agent and preparation method thereof

A technology of ultrasonic contrast agent and polymer, which is applied in echo/ultrasonic imaging agents, pharmaceutical formulations, preparations for in vivo tests, etc. It can solve the problems of easy breakage of contrast agents, poor target loading capacity, and wide particle size. The effects of long contrast duration, long storage time, high pressure resistance and stability

Pending Publication Date: 2020-02-11
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the above problems, the present invention provides a method for preparing a porous polymer ultrasonic contrast agent, which mainly solves the problems that the contrast agent prepared in the prior art is easy to break, has a wide particle size, and has poor target loading ability.

Method used

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  • Porous polymer ultrasound contrast agent and preparation method thereof
  • Porous polymer ultrasound contrast agent and preparation method thereof
  • Porous polymer ultrasound contrast agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] A platelet-coated macromolecule-targeted ultrasound contrast agent is prepared by weighing 500mg of PLGA and dissolving it in 10mL of dichloromethane, and completely dissolving it;

[0054] Take 50ml of 4% PVA in 250ml beaker A, take 96ml of double distilled water in beaker B, add 4ml of isopropanol to beaker B;

[0055] Take by weighing 50mg camphor and add in the dichloromethane that is dissolved with PLGA;

[0056] Weigh 20mg of sodium bicarbonate and dissolve it in 2ml of water to prepare 1% ammonium bicarbonate solution;

[0057] Primary emulsification: Take 2ml of 1% ammonium bicarbonate solution and add it to the solution of the above 3 steps, and process it with a cell disruptor for 1min (power 150W on for 2s / off for 1s), and the emulsion with good colostification should be a uniform milky white liquid;

[0058] Double emulsification: Pour the liquid after primary emulsification into the above-mentioned 4% PVA beaker A, and process it with a homogenizer for 3 m...

Embodiment 2

[0083] Weigh 500mg PLGA and dissolve it in dichloromethane, the ratio of the two is 1:26, and dissolve completely;

[0084] Take 50ml of 4% PVA in 250ml beaker A, take 96ml of double distilled water in beaker B, add 4ml of isopropanol to beaker B;

[0085] Weigh 50 mg of camphor and add it to dichloromethane dissolved in PLGA, the mass ratio of camphor and PLGA is 1:8;

[0086] Weigh 20mg of sodium bicarbonate and dissolve it in 2ml of water to prepare 1% ammonium bicarbonate solution;

[0087] Primary emulsification: Take 2ml of 1% ammonium bicarbonate solution and add it to the solution of the above 3 steps, and process it with a cell disruptor for 1min (power 150W on for 2s / off for 1s), and the emulsion with good colostification should be a uniform milky white liquid;

[0088] Double emulsification: Pour the liquid after primary emulsification into the above-mentioned 4% PVA beaker A, and process it with a homogenizer for 3 minutes, 10000rmp / min;

[0089] Pour the 4% isop...

Embodiment 3

[0095] Weigh 500mg PLGA and dissolve it in dichloromethane, the ratio of the two is 1:27, and dissolve completely;

[0096] Take 50ml of 4% PVA in 250ml beaker A, take 96ml of double distilled water in beaker B, add 4ml of isopropanol to beaker B;

[0097] Weigh 50 mg of camphor and add it to dichloromethane dissolved in PLGA, the mass ratio of camphor and PLGA is 1:8;

[0098] Weigh 20mg of sodium bicarbonate and dissolve it in 2ml of water to prepare 1% ammonium bicarbonate solution;

[0099] Primary emulsification: Take 2ml of 1% ammonium bicarbonate solution and add it to the solution of the above 3 steps, and process it with a cell disruptor for 1min (power 150W on for 2s / off for 1s), and the emulsion with good colostification should be a uniform milky white liquid;

[0100] Double emulsification: Pour the liquid after primary emulsification into the above-mentioned 4% PVA beaker A, and process it with a homogenizer for 3 minutes, 8000rmp / min;

[0101] Pour the 4% isopr...

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Abstract

The invention relates to a porous polymer ultrasound contrast agent and a preparation method thereof. The preparation method comprises the following steps: S1: dissolving the polylactic-co-glycolic acid in dichloromethane; S2: taking camphor and adding camphor into the dichloromethane solution dissolving the polylactic-co-glycolic acid; S3: performing primary emulsion, specifically adding ammoniumbicarbonate solution into the solution obtained by S2, and performing crushing treatment; S4: performing secondary emulsion, mutually dissolving the solution obtained in S3 and polyvinyl alcohol solution and performing homogenizing; S5: mutually dissolving the solution obtained in S4 and isopropanol solution and performing homogenizing; S6: centrifugally collecting samples and freezing the samples; and S7: performing cell membrane coating, specifically mutually mixing a cell membrane suspension and a finished product obtained in S6 and performing processing by an ultrasonic cleaning machine to obtain a finished product. The beneficial effects are: the contrast agent is stronger, has high compression resistance and stability. Under the mechanical index of conventional ultrasound examination, the contrast agent is not relatively liable to be destroyed, has a long duration of radiography, and the cell membrane coating makes the contrast agent targeted, so the contrast agent can be used as an ultrasound molecular imaging contrast agent.

Description

technical field [0001] The invention relates to the technical field of contrast agents, in particular to a preparation method of a porous polymer ultrasonic contrast agent. Background technique [0002] In recent years, among various imaging examination methods, ultrasound imaging has been widely used clinically because of its safety, timely, non-invasive, fast, and low cost. Therefore, ultrasound molecular imaging has also received extensive attention. [0003] Ultrasound image generation relies on the reception, analysis, and display of reflected and scattered ultrasound signals. As a kind of medical imaging, it provides important functional and anatomical information of internal organs, and is a wide range of non-invasive detection methods. Ultrasonic Doppler technology evaluates the direction and velocity of blood flow, which improves the diagnostic ability of ultrasound. In order to better obtain accurate information of specific tissues, the imaging method of ultrasoun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/22
CPCA61K49/223
Inventor 谢明星陈逸寒杨亚利徐玲玲
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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