Male-promoting gene MmS for populus deltoides and application thereof
A gene and gene carrier technology, which is applied to the male-promoting gene MmS of P. nigra and its application field, can solve the problems of a large number of pollen, environmental pollution, and the fact that the sex-determining gene has not been cloned and determined, so as to promote the development of male flowers and solve the problem of pollen pollution. Effect
Active Publication Date: 2020-02-11
NANJING FORESTRY UNIV
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Problems solved by technology
And the poplar male plant will produce a large amount of pollen after reaching the age of sexual maturity, causing serious environmental pollution
Therefore, the important influence of sex should be fully considered when using poplar for afforestation or greening, but the sex-determining genes of poplar have not been cloned and determined
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Embodiment 1
[0030] (1) Acquisition of the male-specific MmS gene of Populus nigra
[0031] The sex-determining genes of Populus nigra were mapped using the full-sib population of P. americana, and the sex-determining genes were located at the end of the nineteenth chromosome. Sex linkage analysis found that the sex-determining genes of poplar were heterozygous for males, indicating that the sex determination system of Populus nigra was XY. Referring to the genome sequence of Populus americana male strain, we designed SSR markers in the sex-determining region, and then carried out fine mapping of the local region of the sex-determining gene region, and performed haplotype construction on the sex-determining gene region. There is a Y-specific hemizygous segment on the body. Whole-genome resequencing was carried out using the collected germplasm materials of natural populations of Populus americana. Using resequencing data, we carried out an association analysis between genome-wide Read co...
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The invention discloses a male-promoting gene MmS for populus deltoides and application thereof, and belongs to the technical field of plant genetic engineering. The specific male-promoting gene MmS of the populus deltoides disclosed by the invention does not encode a protein, but rather as a miRNA sponge for adsorbing miRNA; and through reducing concentration of the adsorbed free miRNA, an expression level of a down-stream target gene is up-regulated. The MmS gene exists in a male plant of the populus deltoides in a form of hemizygote, and only exists on a Y chromatid; and an X chromatid doesnot contain the gene. The male-promoting gene makes a male flower of the male plant of the populus deltoides to be developed, and the male plant of the populus deltoides does not contain the gene, and therefore, a male flower organ of the male plant of the populus deltoides is not developed. A large amount of pollen can be produced after the male plant of the populus deltoides reaches sexual maturity, so that severe environmental pollution is caused; and the MmS gene is knocked out in the male plant of the populus deltoides, so that a new variety of the populus deltoides which cannot producethe pollen can be cultured, the gene also can be imported into the plant, the development of male flowers is promoted, and thus, the gene has an important application value in seed cultivation of thepopulus deltoides.
Description
technical field [0001] The invention belongs to the technical field of plant genetic engineering, and more specifically relates to a male-promoting gene MmS of black poplar (Poplus deltoides) and its application. Background technique [0002] The hypothesis of the origin of plant sex chromosomes holds that sex chromosomes originate from a pair of autosomes. The evolution of the sex chromosomes begins with a male or female sterile mutation on one chromosome of the pair of autosomes. The hypothesis of the origin of plant sex chromosomes holds that sex chromosomes originate from a pair of autosomes. The evolution of sex chromosomes begins with a male or female sterile mutation on one chromosome of the pair of autosomes. Due to some unknown reason, the recombination of the mutation site is inhibited, and the non-recombination region gradually expands to form a heterozygous sex chromosome. Dioecious plants are evolutionarily shorter than closely related monoecious plants, from ...
Claims
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Login to View More IPC IPC(8): C12N15/29C12N15/82A01H5/02C12Q1/6895
CPCC07K14/415C12N15/827C12Q1/6895C12Q2600/13C12Q2600/158
Inventor 尹佟明吴怀通薛良交陈赢男李小平侯静戴晓港陆静
Owner NANJING FORESTRY UNIV