A kind of hydrolysis method of zearalenone and derivatives thereof

A technology for zearalenone and zearalenol is applied in the field of hydrolysis of zearalenone and its derivatives, and can solve problems such as low relative activity
CN110819608BActive Publication Date: 2022-03-15HUBEI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HUBEI UNIV
Publication Date
2022-03-15

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Abstract

The invention relates to a method for hydrolyzing zearalenone and its derivatives, using zearalenone or its derivatives as a substrate, and using a zearalenone degrading enzyme to hydrolyze the substrate, the The amino acid sequence of zearalenone degrading enzyme is shown in SEQ ID NO: 1 or SEQ ID NO: 2. The zearalenone degrading enzyme of the present invention has high enzyme activity, good temperature and pH tolerance, etc. Advantages, can be widely used in the enzymatic hydrolysis of zearalenone and several derivatives, with a wide range of substrates, among which it has high activity on zearalenone, α-zearalenol and β-zearalenol , SEQ ID NO: 2 mutates the 158th T to H in the amino acid sequence shown in SEQ ID NO: 1, and after the mutation, its enzyme activity for the substrate α-zearalenol is increased by 40%.
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Description

technical field

[0001] The invention relates to the field of biological technology, in particular to the application of a zearalenone degrading enzyme in hydrolyzing zearalenone and its derivatives and a method for hydrolyzing zearalenone and its derivatives. Background technique

[0002] Zearalenone, first isolated from corn, is a non-steroidal estrogenic mycotoxin that can be produced by many Fusarium species, both before and after harvest. Zearalenone is consistently found in many crop and cereal by-products including corn, barley, wheat, etc., especially in environments that are suitable for fungal growth.

[0003] There are many derivatives of zearalenone, such as zearalenol, which will enter the food chain through contaminated crops and accumulate in human and animal bodies, causing damage to organisms. The chemical structure of zearalenone and its derivatives is similar to natural estrogen, so they can competitively bind to estrogen receptors, causing external and in...

Claims

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