Cudrania tricuspidata breeding method using hard branch cuttage

A technology of hard branch cutting and zhe wood, applied in the breeding field of zhe wood hard branch cutting, can solve problems such as limited resources and the like

Inactive Publication Date: 2020-02-25
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The main chemical components in Zheshu medicinal materials are flavonoids and ketones, but their resources are limited

Method used

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  • Cudrania tricuspidata breeding method using hard branch cuttage
  • Cudrania tricuspidata breeding method using hard branch cuttage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Material: annual Zhe wood branches;

[0028] Treatment: Soak with 200mg / L IBA solution (i.e. indole butyric acid solution) for 8 hours;

[0029] Protocol: Three biological replicates, 50 cuttings per replicate;

[0030] Specific operation:

[0031] 1. Take the vigorous annual Zhe wood branches, remove all the leaves on the branches, and cut the branches into 8 long sections;

[0032] 2. Bundle 50 stems cut into small sections and tie them with rubber bands;

[0033] 3. Prepare 1L of 200mg / L IBA solution;

[0034] 4. Soak the pruned branches in 200mg / L IBA solution for 8 hours;

[0035] 5. Prepare the matrix for cuttings, mix perlite: vermiculite: peat according to the volume ratio of 1:1:1, and then put it into a 50-hole seedling tray.

[0036] 6. Insert the soaked branches in step 4 into the prepared seedling trays in step 5, and insert the 2 cm of the base of the branches into the matrix of the trays.

[0037] 7. After the cuttings are placed in a solar greenhou...

Embodiment 2

[0046] Material: annual Zhe wood branches;

[0047] Treatment: Soak in 200mg / L IBA for 8h;

[0048] Protocol: Three biological replicates, 50 cuttings per replicate;

[0049] Cutting substrate: The substrate block was purchased from Pinshi Top Gardening (Shanghai) Co., Ltd. The substrate block is a cylinder with a diameter of 5 cm and a height of 4 cm. The components are peat and coconut peat with a volume ratio of 3:1;

[0050] Specific operation:

[0051] 1. Take the robust annual Zhe wood branches, remove all the leaves on the branches, and cut the branches into 8cm long sections with branch clippers;

[0052] 2. Bundle 50 stems cut into small sections and tie them with rubber bands;

[0053] 3. Prepare 1 liter of 200mg / L IBA solution;

[0054] 4. Soak the pruned branches in 200mg / L IBA solution for 8 hours;

[0055] 5. Put the matrix block into a 50-hole tray;

[0056] 6. Insert the soaked branches in 4 into the prepared seedling trays in 5, and insert the base of th...

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Abstract

The invention provides a Cudrania tricuspidata breeding method using hard branch cuttage and belongs to the technical field of asexual breeding. The method includes: soaking Cudrania tricuspidata branch bases into an indolebutyric acid solution to obtain soaked branches, and culturing the soaked branches in a culture medium blocks in a cuttage manner, wherein the concentration of the indolebutyricacid solution is 200mg/L, and the culture medium blocks comprise turf and coconut coir. According to the embodiments of the invention, by the method, rooting rate can reach 85.66%, adventitious rootnumber can reach 24.33/branch, and the survival rate of seedlings transplanted into a field can reach 99.02%.

Description

technical field [0001] The invention belongs to the technical field of vegetative propagation, in particular to a method for propagating hardwood cuttings of eucalyptus. Background technique [0002] Zhe wood is a plant of the genus Zhe in the family Moraceae, also known as Zhe tree, thorn mulberry, Zhe mulberry, yellow mulberry, slave Zhe, oak tree and so on. Deciduous shrubs or small trees, which like to grow in sunny barren mountains, slopes, hills and beside water streams, are widely distributed in East China, Central South, Southwest to southern Hebei. The roots, bark or root bark, stems and leaves, and fruits of the tree can be used as medicine. It has the effects of dispelling wind and dampness, promoting blood circulation and dredging menstruation, relieving cough and reducing phlegm, and can treat rheumatic joint pain, jaundice, stranguria, amenorrhea, and traumatic injuries Injuries and boils and carbuncles have been used in the treatment of digestive system tumor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G2/10A01G24/28A01G24/25
CPCA01G2/10A01G24/25A01G24/28
Inventor 徐晓召苏升辉程晨霞
Owner QINGDAO AGRI UNIV
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