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Fully human antibody or antibody fragment against CD19, chimeric antigen receptor and application

A technology of chimeric antigen receptor and fully human antibody, applied in the direction of anti-receptor/cell surface antigen/cell surface determinant immunoglobulin, application, receptor/cell surface antigen/cell surface determinant, etc., can Solve the problems of poor prognosis and high recurrence rate

Active Publication Date: 2021-07-09
CHANGZHOU VELOX PHARMA SCI & TECH CO LTD
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AI Technical Summary

Problems solved by technology

[0002] B-cell malignancies are common hematological malignancies. Although the first-line clinical treatment has a certain effect, the recurrence rate is high and the prognosis is poor. CD19, as a molecular target for immunotherapy of B-cell malignancies, has received great attention

Method used

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  • Fully human antibody or antibody fragment against CD19, chimeric antigen receptor and application
  • Fully human antibody or antibody fragment against CD19, chimeric antigen receptor and application
  • Fully human antibody or antibody fragment against CD19, chimeric antigen receptor and application

Examples

Experimental program
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Effect test

Embodiment

[0091] 1. Construction of directional anti-CD19 fully human antibody sub-library

[0092] 1.1 Using phage display technology, the anti-human CD19 specific antibody sub-library was screened based on the fully human natural antibody library independently constructed by Fellowes.

[0093] (1) Establish a phage antibody library of fully human single-chain antibodies:

[0094] Design primers to amplify the heavy chain variable region and light chain variable region of the antibody, connect the heavy chain variable region and light chain variable region with Linker by overlapping extension PCR, and obtain the full-length PCR product, use SfiI Digest the PCR product and the pCGMT3 phagemid vector, electrotransform the ligated transformation product into XL1-blue competent cells, then add 3 mL of SOC medium to the competent cells, incubate at 30°C for 1 hour, add a final concentration of 50 μg / mL of ampicillin, 20 mL of 10 μg / mL tetracycline solution, and shake at 37°C for 2 hours. ...

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PUM

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Abstract

The invention discloses a fully human antibody or antibody fragment against CD19, a chimeric antigen receptor and applications. The antibody or antibody fragment or nucleic acid encoding the antibody or antibody fragment can be used to prepare host cells, lentiviral plasmids, diagnose tumors, and targeted antitumor drugs. The antibody or antibody fragment of the present invention can bind to human CD19 protein with high specificity.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to fully human antibodies or antibody fragments against CD19 and applications thereof. Background technique [0002] B-cell malignancies are common hematological malignancies. Although the first-line clinical treatment has certain curative effect, the recurrence rate is high and the prognosis is poor. As a molecular target for immunotherapy of B-cell malignancies, CD19 has received great attention. CD19 is a specific surface protein of normal and malignant B lymphocytes, which plays an important role in the development, proliferation and differentiation of B cells as well as malignant transformation. Therefore, the specificity of CD19 expression in B lymphocytes and the widespread expression of malignant tumors make it a potential molecular target for immunotherapy of B lymphocyte malignant tumors. [0003] Various immunotherapy strategies targeting CD19 are being developed in ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/28C07K19/00C12N5/10C12N15/867G01N33/574A61K39/00A61P35/00
CPCC07K16/2803C07K14/7051A61K39/0011A61P35/00C07K2319/02C07K2319/03C07K2319/33C07K2317/622C12N2740/15043
Inventor 杨光
Owner CHANGZHOU VELOX PHARMA SCI & TECH CO LTD
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