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Method for detecting dibutyl phthalate through fluorescence ratio method based on dual-emission carbon quantum dot marked nucleic acid aptamer

A technology of dibutyl phthalate and nucleic acid aptamer, which is applied in the detection field of dibutyl phthalate and dibutyl phthalate by fluorescence ratio method, and can solve difficult fluorescent labeling and tracking, Problems such as poor photostability, photobleaching and high toxicity, achieve rapid screening, simple preparation method, and improve selectivity and sensitivity

Active Publication Date: 2020-02-28
SHANDONG AGRICULTURAL UNIVERSITY
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Problems solved by technology

Nucleic acid aptamers are often used in combination with fluorescent labeling materials in practical operations to achieve sensitive and intuitive detection purposes. However, traditional organic fluorescent dyes and fluorescent proteins have disadvantages such as poor photostability, photobleaching and high toxicity, making them difficult to use. For long-term fluorescent labeling and tracking

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  • Method for detecting dibutyl phthalate through fluorescence ratio method based on dual-emission carbon quantum dot marked nucleic acid aptamer
  • Method for detecting dibutyl phthalate through fluorescence ratio method based on dual-emission carbon quantum dot marked nucleic acid aptamer

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Embodiment 1

[0054] 1. Preparation and activation of dual emission carbon quantum dots:

[0055] (1) Preparation of double emission carbon quantum dots: add 0.8 g of ascorbic acid to 13 mL of methanol and 7 mL of water, stir vigorously for 1 min, put the solution in a reaction kettle, and heat at 160° C. for 70 min. After cooling to room temperature, the solution was passed through a 0.22 μm filter membrane to remove larger aggregates, and finally a double-emission carbon quantum dot solution was obtained, which was stored away from light.

[0056] (2) Activation of double-emission carbon quantum dots: Dilute the double-emission carbon quantum dot solution obtained in step (1) with water, and the dilution ratio of carbon quantum dot solution and water is 1.2:30; take 500 μ L of diluted double-emission carbon quantum dots Mix the dots with 1mL water and 20mgEDC evenly, and react in the dark for 20min at room temperature, then slowly add 30mg NHS and gently mix and incubate for 10min to obta...

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Abstract

The invention discloses a method for detecting dibutyl phthalate through a fluorescence ratio method based on dual-emission carbon quantum dot marked nucleic acid aptamer. Sensitive detection of dibutyl phthalate is realized by taking dual-emission carbon quantum dots as a marker and combining with an aminated nucleic acid aptamer specifically recognizing dibutyl phthalate; the method is low in detection limit, high in specificity, stable in fluorescent performance and suitable for ultrasensitive quick detection of dibutyl phthalate in food.

Description

technical field [0001] The invention relates to a detection method of dibutyl phthalate, in particular to a method for detecting dibutyl phthalate by a fluorescence ratio method based on double-emitting carbon quantum dot-labeled nucleic acid aptamers, which belongs to food safety detection technology field. Background technique [0002] Dibutyl phthalate is an important chemical plasticizer, widely used in plastic products, cosmetics and food packaging materials and other materials. The plasticizer dibutyl phthalate can greatly increase the plasticity and strength of plastics, and its content can sometimes reach 50% of the product. Therefore, dibutyl phthalate is ubiquitous in packaging materials. There are a lot of contacts. However, dibutyl phthalate has been identified as the fourth type of toxic chemical substance, which can enter the human body through the respiratory tract, digestive tract and skin, and because the ortho-phthalate plasticizer is only physically comb...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6486
Inventor 徐志祥王汐沫陈晨孔非凡
Owner SHANDONG AGRICULTURAL UNIVERSITY
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