Method for detecting dibutyl phthalate through fluorescence ratio method based on dual-emission carbon quantum dot marked nucleic acid aptamer
A technology of dibutyl phthalate and nucleic acid aptamer, which is applied in the detection field of dibutyl phthalate and dibutyl phthalate by fluorescence ratio method, and can solve difficult fluorescent labeling and tracking, Problems such as poor photostability, photobleaching and high toxicity, achieve rapid screening, simple preparation method, and improve selectivity and sensitivity
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[0053] Example 1:
[0054] 1. Preparation and activation of dual emission carbon quantum dots:
[0055] (1) Preparation of dual emission carbon quantum dots: 0.8 g of ascorbic acid was added to 13 mL of methanol and 7 mL of water, and after vigorous stirring for 1 min, the solution was placed in a reactor and heated at 160°C for 70 min. After cooling to room temperature, the solution was passed through a 0.22μm filter membrane to remove larger agglomerates, and finally a solution of dual emission carbon quantum dots was obtained and stored in the dark.
[0056] (2) Activation of dual emission carbon quantum dots: Dilute the dual emission carbon quantum dot solution obtained in step (1) with water, and the dilution ratio of the carbon quantum dot solution to water is 1.2:30; take 500 μL of the diluted dual emission carbon quantum dot The dots were mixed with 1 mL of water and 20 mg of EDC uniformly, and reacted for 20 minutes in the dark at room temperature, and then slowly added 30 ...
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