Automatic film washing device for Western blot experiment

Abstract

The invention relates to the technical field of Western blot experiments, and discloses an automatic film washing device for a Western blot experiment. The automatic film washing device comprises a shell, a first liquid storage tank, a second liquid storage tank, a third liquid storage tank and a fourth liquid storage tank, wherein the first liquid storage tank, the second liquid storage tank, thethird liquid storage tank and the fourth liquid storage tank are arranged at the top in the shell and used for injecting liquid into a washing device. The washing device is arranged below the liquidstorage devices and comprises a washing disc, liquid leakage discs coaxial with the washing disc are sequentially arranged below the washing disc, and the washing disc and the liquid leakage discs canrotate relatively. Liquid leakage holes are formed in the washing disc and the liquid leakage discs, and the washing disc and the liquid leakage discs can vibrate along with a shaft rod. A first recycling box, a second recycling box, a third recycling box and a fourth recycling box are arranged below the liquid leakage discs from an outer ring to an inner ring. Automation of experiment steps after film rotation can be achieved, and meanwhile liquid can be recycled in a classified mode for repeated utilization.

Description

technical field [0001] The invention relates to the technical field of Western blot experiments, in particular to an automatic membrane washing device for Western blot experiments. Background technique [0002] Western blotting (immunoblotting test) is Western Blot. It is an experimental method commonly used in molecular biology, biochemistry and immunogenetics. It was proposed by Harry Towbin of the Friedrich Miescher Institute in Switzerland in 1979, and was introduced by Neil Burnett. (Neal Burnette) in 1981 "Analytical Biochemistry" (Analytical Biochemistry) was first called WesternBlot. [0003] Western Blot is to transfer the total protein of cells or tissues separated by electrophoresis from the gel to the solid support NC membrane (nitrocellulose membrane) or PVDF membrane (polyvinylidene fluoride membrane), and then use specific It is a protein detection technology that detects a specific antigen with specific antibodies, and has been widely used in many aspects s...

AI Technical Summary

Problems solved by technology

The intervals between these experimental steps are short and frequent, which brings a lot of inconvenience to researchers.

Although there are some automatic membrane washing devices currently on the market, most of these devices can only complete the TBST membrane washing operation, but cannot program the steps after the membrane transfer as a whole, and cannot completely liberate researchers: and most of these devices are complex and bulky. Huge, expensive to manufacture and difficult to transport

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