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Aspergillus niger SICU-33 capable of promoting plant growth and application thereof

A technology for promoting plant growth and Aspergillus niger, applied in plant growth regulators, plant growth regulators, applications, etc., can solve the problems of isolation, identification and screening of highly efficient fungal strains, and achieve good market prospects and promote growth. Effect

Active Publication Date: 2020-03-06
SICHUAN AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on PGPR strains is mainly focused on the study of PGPR bacteria and actinomycetes, but the isolation, identification and screening of highly efficient fungal strains with PGPR function are still rare.

Method used

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  • Aspergillus niger SICU-33 capable of promoting plant growth and application thereof
  • Aspergillus niger SICU-33 capable of promoting plant growth and application thereof
  • Aspergillus niger SICU-33 capable of promoting plant growth and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The isolation and identification of embodiment 1 Aspergillus niger SICU-33

[0020] 1.1 Source of strain

[0021] The soil in this example was taken from Yeba Village, Longzi Town, Longzi County, Tibet Autonomous Region. The rhizosphere soil of highland barley was randomly collected, put into sterilized sealed plastic bags, labeled, brought back to the laboratory in time, and stored in a -20°C refrigerator.

[0022] 1.2 Isolation of soil fungi

[0023] After the thawed soil samples were serially diluted 10 times, they were inoculated on the Martin’s medium plate, cultured upside down at 28°C for 7 days, single colonies were picked and purified according to the colony’s size, shape, color and other appearance characteristics, and obtained by separation 90 strains with different colony morphology.

[0024] 1.3 Identification of Aspergillus niger SICU-33

[0025] The fungal DNA was extracted with a fungal genome extraction kit, diluted 50 times as a template for PCR re...

Embodiment 2

[0027] Embodiment 2 produces indole acetic acid (IAA) and measures

[0028] Inoculate the 90 strains obtained above into King's Medium and culture them to the logarithmic phase, shake and culture at 140r / min at 28°C for 3 days, take 50μl on a cleaned white porcelain plate, add 50μL of chromogen, and put them at 28°C Protect from light for 30 minutes, if pink appears, it indicates that IAA is produced, and King's medium without inoculation is used as a control.

[0029] Centrifuge the strains cultured in King's Medium at 8000r / min for 5min, take 5ml of the supernatant and add 5ml of Salkowski chromogen, and measure the absorbance at 530nm after 30min at 28°C in the dark. The blank medium was used as a control, and the optical density corresponding to the standard sample of IAA was used as a standard curve to calculate the output (mg / L) of IAA. The IAA production of different strains is shown in Table 1.

[0030] Table 1 The ability of fungi to produce IAA in the rhizosphere of...

Embodiment 3

[0034] Embodiment 3 antibacterial activity assay

[0035] The antibacterial effects of the isolated strains on three indicator bacteria, Fusarium wilt of wheat, Fusarium wilt of watermelon and Magnaporthe grisea were determined by plate confrontation method. The diameter of the inhibition zone (HD) and the diameter of the colony (CD) were measured, and the strains with antibacterial effect were screened out by calculating the ratio of HD / CD, as shown in Table 2.

[0036] Table 2 Antibacterial ability of cultivable fungi in the rhizosphere soil of highland barley

[0037]

[0038]

[0039] Among them, the indicator strains: 1: Fusarium rubella wheat; 2: Fusarium wilt of watermelon; 3: Magnaporthe grisea. +++: HD / CD value is between 3-4; ++: HD / CD value is between 2-3 ;+: HD / CD value between 1–2; -: HD / CD value less than 1.

[0040] It can be seen from Table 2 that 10 strains have antagonistic effects on wheat head blight, 24 strains have antagonistic effects on watermel...

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Abstract

The invention discloses Aspergillus niger SICU-33 capable of promoting plant growth and application thereof. The preservation number of the Aspergillus niger SICU-33 disclosed by the invention is CCTCC NO: M2019778, and the ITS sequence of the Aspergillus niger SICU-33 is SEQ NO.1. The strain not only can obviously inhibit the growth of plant pathogenic fungi, but also can promote the growth of plants, increases the leaf number, stem diameter, maximum leaf width, maximum leaf length, fresh leaf weight, fresh root weight, root length and leaf area of corn, and has a good application prospect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to an Aspergillus niger strain, in particular to Aspergillus niger SICU-33 for promoting plant growth and an application thereof. Background technique [0002] Highland barley is a barley crop of the Gramineae family, a type of naked barley. Tibet is the main area where highland barley is grown. The pH of Tibetan highland barley planting soil is relatively high. The pH value of the soil in most areas is between 7.6-8.5, which is higher than the optimal growth of highland barley. pH needs; in addition, the sunshine time in Tibet is long, the water evaporates quickly, and the soil quality and fertility are generally poor. 67.89% of the farmland soil is sandy loam. Therefore, fertilizing the soil fertility of highland barley planting soil in Tibetan areas is of great significance for improving the yield and quality of highland barley, meeting the food needs of Tibetan people, and further imp...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/34A01P21/00A01P3/00A01G7/06A01G13/00C12R1/685
CPCA01G7/06A01G13/00C12N1/145C12R2001/685
Inventor 辜运富王文仙尼玛扎西高雪普布贵吉向泉桔赵珂余秀梅陈强廖德聪
Owner SICHUAN AGRI UNIV
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