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Application of protein marker or a combination thereof in prognosis judgment of colorectal cancer

A colorectal cancer and prognosis judgment technology, applied in the field of molecular biology, can solve the problems of inaccurate judgment of prognosis of CRC patients, high risk of recurrence and metastasis, and inability to distinguish

Active Publication Date: 2020-03-06
CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Based on clinical indicators such as TNM stage, the prognosis of CRC patients cannot be accurately judged, and it is impossible to distinguish which stage II patients and stage III patients have a higher risk of recurrence and metastasis, so it is not clear which patients may benefit from adjuvant therapy

Method used

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  • Application of protein marker or a combination thereof in prognosis judgment of colorectal cancer
  • Application of protein marker or a combination thereof in prognosis judgment of colorectal cancer
  • Application of protein marker or a combination thereof in prognosis judgment of colorectal cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0100] Example 1: Study on the correlation between the expression levels of p-mTOR, MAP4 and DNAJB6 and the clinicopathological parameters of colorectal cancer

[0101] (1) For 73 cases of colorectal cancer tissue samples confirmed by pathological detection (most cases have only colon cancer or rectal cancer, but some patients have both colon cancer and rectal cancer), use the above-mentioned IHC method to detect p-mTOR and MAP4 and DNAJB6 protein expression levels, and judge whether the expression level is high expression or low expression. The test results and the clinicopathological parameters of the patients are shown in Table 1 below.

[0102] Table 1: Correlation of expression levels of p-mTOR, MAP4 and DNAJB6 with clinicopathological parameters of colorectal cancer

[0103]

[0104]

[0105] in FIG. 1:

[0106] * indicates Fisher's exact test,

[0107] P values ​​are marked in italics;

[0108] According to the degree of differentiation of colorectal tumor c...

Embodiment 2

[0113] Example 2: Correlation study between the expression levels of p-mTOR, MAP4 and DNAJB6 and the postoperative overall survival of patients

[0114] The protein expression of colorectal cancer and the overall survival time of patients after surgery are shown in Table 2 below and attached Figure 2A -2D.

[0115] Table 2: Colorectal cancer protein expression and postoperative overall survival data

[0116]

[0117]

[0118]

[0119] Combined with the data in Table 1 and the overall survival time data of the patients (Table 2), multivariate Cox regression analysis was performed.

[0120] The specific method of univariate survival analysis of the Kaplan-Meier method: first calculate the probability (survival probability) of patients who have survived for more than a certain period (1 month) and then survive the next period (1 month), and then calculate the survival probability one by one. Multiplying the probabilities gives the survival rate for the corresponding...

Embodiment 3

[0129] Example 3: Correlation between the expression levels of p-mTOR, MAP4 and DNAJB6 and the median survival time of CRC patients

[0130] According to the expression levels of p-mTOR, MAP4 and DNAJB6 detected by IHC method, combined with the postoperative survival time of patients, the relationship between the expression levels of individual markers and marker combinations in CRC patients was analyzed by life table method and weighted regression method. The relationship between bit survival time.

[0131] The median survival time, also known as the median survival time, represents the time at which exactly 50% of the individuals have not yet survived. The longer the median survival time, the better the prognosis of the disease; the shorter the median survival time, the worse the prognosis.

[0132] The specific method of analyzing the median survival time by the life table method: Divide the survival time into many small time periods, calculate the change of the survival...

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Abstract

The invention belongs to the fields of molecular biology and clinical detection, and relates to application of a protein marker or a combination thereof in prognosis judgment of colorectal cancer. Specifically, the invention relates to use of any one item selected from following (1)-(7) items in preparation of medicines for diagnosis, assessment of recurrence risk, prognostic judgment or assistance therapy of colorectal cancer (such as colorectal cancer stage II or stage III), wherein the(1)-(7) items are (1) p-mTOR, (2) p-mTOR, MAP4, (3) p-mTOR, DNAJB6, (4) MAP4, (5) MAP4, DNAJB6, (6) p-mTOR,MAP4, DNAJB6 and (7) reagents, and any one item of the (1)-(6) items can be detected (such as quantitative detection or qualitative detection). The marker or the marker combination can be effectivelyused for judging the prognosis of patients with colorectal cancer, such as the prediction of postoperative survival time and median survival time of the patients.

Description

technical field [0001] The invention belongs to the fields of molecular biology and clinical detection, and relates to the application of protein markers or combinations thereof in the prognosis judgment of colorectal cancer. Background technique [0002] Colorectal cancer (CRC) is one of the most common malignant tumors of the digestive system. About 1.2 million patients worldwide are diagnosed with colorectal cancer every year, and more than 600,000 patients die directly or indirectly from colorectal cancer. In developed countries in Europe and the United States, colorectal cancer is a malignant tumor with the third highest incidence rate and the second highest mortality rate. In my country, the incidence of colorectal cancer has been increasing year by year in recent years. [0003] Currently, surgery is the main treatment for CRC, and postoperative recurrence and metastasis are the main causes of CRC treatment failure and patient death. The prognosis of patients with ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 王明荣张钰郝佳洁张彤彤梁建伟王征徐昕蔡岩朱依青
Owner CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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