Anti-amantadine (Anti-AMD) single-chain antibody scFv, and preparation method and application thereof

A single-chain antibody and amantadine technology, which is applied in the field of amantadine AMD single-chain antibody scFv and its preparation, and can solve problems such as unreported, unreported patents, etc.
CN110872355AActive Publication Date: 2020-03-10CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2020-03-10

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Abstract

The invention discloses an anti-amantadine AMD single-chain antibody, and a preparation method and application thereof. According to the invention, a heavy-chain variable region gene and a light-chainvariable region gene of the antibody can be obtained from a hybridoma cell strain 3F2 of a monoclonal antibody secreting amantadine (AMD) through a genetic engineering mean; and after the two genes are recombined, a prokaryotic expression plasmid vector is constructed, and escherichia coli is transformed to express scFv antibody active fragments capable of specifically recognizing the AMD. Experiments prove that the recombinant single-chain antibody scFv prepared by the method has specific recognition and binding activity withamantadine. Residual detection of amantadine can be realized through utilization of recognition and binding activity of the recombinant single-chain antibody on amantadine, and the constructed transformation vector plasmid is easy for storage and mass-produce.
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Description

technical field

[0001] The present invention relates to an amantadine AMD single-chain antibody scFv and a preparation method thereof, in particular to the protein sequence of the single-chain antibody and its coding gene sequence, a vector containing the coding gene and a method for preparing the single-chain antibody , belonging to the field of biological products. Background technique

[0002] Amantadine and other antiviral drugs are used in the prevention and treatment of influenza A virus in animal husbandry, but the abuse of amantadine is likely to cause drug resistance, promote strain mutation and endanger human health, and has a series of neurotoxicity, etc. Side effects, such as causing nervousness, anxiety, nightmares and other symptoms. Announcement No. 560 of the Ministry of Agriculture of my country in 2005 clearly stipulates that antiviral drugs such as amantadine and rimantadine are prohibited from being used in veterinary medicine. Livestock and poultry. Th...

Claims

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