Use of compound in treatment of asthenozoospermia

A compound and sperm technology, applied in the preservation of human or animal body, medical preparations containing active ingredients, and applications, can solve the problems of sperm motility not as good as fresh semen, low fertilization rate, etc.

Active Publication Date: 2020-03-17
上海市生物医药技术研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although semen cryopreservation technology is widely used, the sperm motility of semen after freezing-thawing process is far less than that of fresh semen, and the fertilization rate is often lower than that of natural mating

Method used

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  • Use of compound in treatment of asthenozoospermia
  • Use of compound in treatment of asthenozoospermia
  • Use of compound in treatment of asthenozoospermia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Promoting motility of asthenozoospermia sperm in vitro

[0057] The semen samples were obtained from the sperm of 30 patients with oligoasthenospermia in the outpatient department of the hospital. They were abstinent for 48 hours to 7 days. The sperm was collected by masturbation in a dry and sterile sperm collection cup, and it was completely liquefied within 60 minutes at 37°C. Each semen is placed in a centrifuge tube according to the ratio of semen: medium (BWW) = 1:1, centrifuged at 300g for 15 minutes and then resuspended (final concentration is 5×10^6 individuals / ml), and the resuspension is divided into equal parts For the drug group and the control group, Scriptaid (purchased from Selleck (China, Shanghai Lanmu Chemical Co., Ltd.)) with a final concentration of 10 μM was added to the drug group, and an equal volume of sterilized water was added to the control group. After incubation for 1h, 3h, and 5h at 37°C in a 5% carbon dioxide incubator, sperm parameters (...

Embodiment 2

[0061] Increase the survival time of sperm in culture medium (to the time of motility)

[0062] The sample collection process and grouping were the same as in Example 1, incubated in a 37°C, 5% carbon dioxide incubator, and measured the percentage of motile sperm in the culture with an automatic sperm quality analyzer every 5 hours. A sample was considered no longer viable when there were less than 5% forward motile sperm. The samples were from three different asthenozoospermia patients. See Table 1 for detailed results.

[0063] Table 1

[0064]

[0065] The results showed that the sperm survival time of the drug group was significantly longer than that of the normal group, and Scriptaid can prolong the sperm motility time.

Embodiment 3

[0067] Improve sperm-egg combination and fertilization ability in asthenozoospermia

[0068] Immature female hamsters were intraperitoneally injected with PMSG (60IU / head), and 48 hours later injected with chorionic gonadotropin HCG (100IU / head) to induce ovulation. After 12-15 hours, the female mice were killed to induce ovulation, and the abdominal cavity was opened to take out the fallopian tubes , placed in the balanced BWW, the ampulla of the oviduct was torn apart to allow the cumulus-oocyte complex to flow out, the oviduct and tissue fragments were removed, and hyaluronidase (HY) was added in a proportion of 10%, and the cumulus cells were After falling off, wash 3 times in BWW, transfer to benchtop liquid to remove zona pellucida, wash 3 times in BWW again, and place in 37°C, 5% carbon dioxide incubator for later use. After the male mice were killed, the tail of the epididymis was squeezed with ophthalmic forceps and placed in the BWW to allow the semen to swim out. Th...

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Abstract

The invention discloses an application of a compound in treating asthenozoospermia, which is an application of a compound shown as a structural formula I or pharmaceutically acceptable salt thereof inpreparation of a medicine or a medicine box for promoting sperm motility, prolonging sperm motility time and/or prolonging survival time of unfrozen sperms, and also discloses an application of the compound in preparation of the medicine or the medicine box for assisting reproduction, or in preparation of the medicine or the medicine box for treating asthenozoospermia.

Description

technical field [0001] The invention relates to compounds for promoting sperm motility, prolonging sperm survival time and assisting reproduction and uses thereof. Background technique [0002] At present, there are many assisted reproductive methods for infertility treatment, such as artificial insemination (Artificial Insemination, AI), in vitro fertilization-embryo transfer (IVF) and intracytoplasmic sperm injection (ICSI). These technologies all involve in vitro cultivation and manipulation of sperm. During the process of in vitro cultivation and manipulation, maintaining or even improving the fertilization ability of sperm plays an important role in improving the success rate of assisted reproduction. In internal fertilization, spermatozoa with good motility are required, which can quickly swim up to the fallopian tube to fertilize the egg; in vitro fertilization, it is also essential that sperm with good motility have sufficient power to penetrate the cumulus and zona ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/473A61P15/08A01N1/02C12N5/076
CPCA61K31/473A61P15/08A01N1/0226C12N5/061C12N2500/30
Inventor 于合国杨依婷李玉华刁华
Owner 上海市生物医药技术研究院
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