A method for simultaneous detection of six components in Xiaoer Xuanfei Zhike Granules

A technology of Xuanfei Zhike and granules, which is applied in the direction of measuring devices, material separation, and analysis of materials, can solve problems such as the inability to objectively reflect and evaluate the quality consistency of traditional Chinese medicine, the inability to fully control the internal quality of products, and the need for large manpower and material resources and testing equipment. , to achieve the effect of improving accuracy and sensitivity, large peak area, and fewer instruments

Active Publication Date: 2022-04-05
JIANMIN PHARMA GRP CO LTD
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  • Description
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Problems solved by technology

The above quality control methods have the following defects: (1) the product needs to be extracted and tested several times respectively, resulting in a long testing cycle, requiring a lot of manpower, material resources and testing equipment; (2) only quantitative testing of baicalin is carried out, and other components It can only be qualitative, cannot fully control the internal quality of the product, and cannot objectively reflect and evaluate the consistency of the quality of traditional Chinese medicine

Method used

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  • A method for simultaneous detection of six components in Xiaoer Xuanfei Zhike Granules
  • A method for simultaneous detection of six components in Xiaoer Xuanfei Zhike Granules
  • A method for simultaneous detection of six components in Xiaoer Xuanfei Zhike Granules

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Embodiment 1

[0035] The preparation of embodiment 1 need testing solution

[0036] Before the HPLC content determination, the drug to be tested needs to be extracted. The purpose of the extraction is to reduce the detected impurities in the preparation, including the extracted components in the traditional Chinese medicine that are not related to the detected components, as well as the excipients in the preparation, so as to reduce the impurities as much as possible. The interference of the peak shape of the characteristic peak of the measured object makes the characteristic peak have a better resolution; the second is to extract the component to be tested from the drug as much as possible, thereby increasing the peak area of ​​the characteristic peak and ensuring the accuracy of the detection. According to the prescription and preparation characteristics of Xiaoer Xuanfei Zhike Granules, combined with the solubility, polarity, and stability of the drug ingredients, we respectively investig...

Embodiment 2

[0045] The selection of embodiment 2 detection wavelength

[0046] Detection wavelength: 210nm, 230nm, 250nm, 280nm

[0047] Other conditions are identical with embodiment 1.

[0048] Result: from figure 2 , 5 , 6, 7, it can be seen that when the wavelength is 230nm ( Figure 5 ), except that the chromatographic peak area of ​​amygdalin and baicalin is equivalent to the peak area of ​​210nm, the peak area of ​​several other components is obviously less than the peak area of ​​210nm; when the wavelength is 250nm ( Figure 6 ), the peak areas of the six components were significantly less than the peak area of ​​210nm; when the wavelength was 280nm ( Figure 7 ), the chromatographic peaks of ephedrine hydrochloride at 22.8min were not completely separated, and the peak areas of astragaloside IV at 30.5min and liquiritin at 39.3min were significantly smaller. Considering comprehensively, 210nm is preferred as the optimum wavelength, and the peak separation of the six compone...

Embodiment 3

[0049] The selection of embodiment 3 mobile phases

[0050] (1) A is 0.1% sodium dodecylbenzene sulfonate in acetonitrile-B is 0.1% formic acid solution

[0051] (2) A is 0.01% acetonitrile of sodium dodecylbenzenesulfonate-B is 0.1% formic acid solution

[0052] (3) A is 0.5% sodium dodecylbenzenesulfonate in acetonitrile-B is 0.1% formic acid solution

[0053] (4) A is acetonitrile-B is 0.1% formic acid solution

[0054] (5) A is acetonitrile-B is 0.05mol / L potassium dihydrogen phosphate

[0055] Detection wavelength: 210nm

[0056] Other conditions are identical with embodiment 1.

[0057] Table 2 is the six kinds of component chromatographic peak separation degree calculated according to the HPLC collection of illustrative plates of mobile phase (1)-(4), the HPLC collection of collection of collection of mobile phase (5) sees Figure 8 .

[0058] Table 2 Six components chromatographic peak resolution

[0059]

[0060] As can be seen from the results in Table 2, w...

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Abstract

The invention discloses a method for simultaneously detecting the contents of six components of Xiaoer Xuanfei Zhike Granules, the six components are ephedrine hydrochloride, baicalin, amygdalin, astragaloside IV, liquiritin and acteoside The detection method includes the steps of extracting the Xiaoer Xuanfei Zhike Granules with an extraction solvent, and then applying the extract to a high-performance liquid chromatograph for detection. Compared with the prior art, the invention has the advantages of less time-consuming detection, high speed, low cost and less instruments, greatly improves the detection efficiency, and can more comprehensively control the internal quality of the traditional Chinese medicine.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine detection, and relates to a method for the content of six components of Xiaoer Xuanfei Zhike Granules. Background technique [0002] CN 102284029A discloses a Xiaoer Xuanfeizhike granule composition and its preparation method and quality control method. Chinese yam, hawthorn, and licorice are prepared with twelve traditional Chinese medicines. It has the effects of clearing the lungs and relieving the exterior, clearing away heat and resolving phlegm. It is mainly used for children with cough due to exogenous pathogenic factors. Coughing up phlegm. [0003] Xiaoer Xuanfei Zhike Granules is a commonly used drug for children with exogenous cough. It has definite clinical curative effect and a large market demand, but its quality standard is simple. TLC qualitative identification was carried out, and the content of baicalin was determined by HPLC method. The above quality control method...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/34G01N30/36G01N30/74
CPCG01N30/02G01N30/34G01N30/36G01N30/74G01N2030/027
Inventor 吴木琴李霞赵刚陈鹏向阳熊登科朱立彬陈骞杨颃高凯李丽娟
Owner JIANMIN PHARMA GRP CO LTD
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