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Application of small molecular citrus pectin in tumor treatment

A small molecule, citrus technology, applied in the field of medicine, can solve the problems of complex development mechanism and achieve the effect of enhancing anti-tumor effect

Pending Publication Date: 2020-03-31
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complex mechanism of tumorigenesis and development, it is necessary to conduct further research on the role of small molecule citrus pectin in tumor-related fields.

Method used

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  • Application of small molecular citrus pectin in tumor treatment
  • Application of small molecular citrus pectin in tumor treatment
  • Application of small molecular citrus pectin in tumor treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] 1. MCP inhibits the differentiation of tumor-associated macrophage TAMs into M2

[0043] (1) Inoculate 1×10 in a 6-well plate 6 For THP-1 cells, add 50nM PMA to make the cells adhere to the wall and differentiate into MO type.

[0044] (2) After 24 hours, add 20ng / ml IL-4 / IL-3 to differentiate the cells into M2 type, and add different concentrations of MCP (0.01-0.1%) at the same time.

[0045] (3) After 72 hours, the M2 cell marker was detected by flow cytometry, and the M2 cell mRNA was detected by PCR.

[0046] It was found that: if figure 1 As shown in (a) and (b), MCP can reduce the expression of markers CD163 and CD68 on the surface of M2 cells, and at the same time inhibit the expression of IL-Ra mRNA and IL-10 mRNA.

[0047] 2. MCP inhibits the survival of TAMs in anoxic environment

[0048] (1) 5000 TAMs cells were inoculated in a 96-well plate, and different concentrations of MCP (0.06%–1%) were added after 24 hours.

[0049] (2) The normoxia group was pl...

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Abstract

The invention provides an application of small molecular citrus pectin in tumor treatment, which belong to the technical field of medicines. Through the research, the condition that small molecular citrus pectin can effectively inhibit differentiation of tumor-associated macrophages (TAMs) into M2 is reported for the first time, meanwhile, the survival of TAMs in an anoxic environment is effectively inhibited, the anti-tumor effect of the bevacizumab can be obviously enhanced by combining with the anti-angiogenesis drug bevacizumab, an experimental foundation is laid for researching the smallmolecular citrus pectin in the field of tumor treatment, a new visual field is provided, and the good practical application value is achieved.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of small molecule citrus pectin in tumor treatment. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Small molecule citrus pectin (Modified Citrus Pectin, MCP) is a natural component extracted from the sponge layer of citrus (such as citrus, lemon, orange and grapefruit, etc.), and modified by enzymes to produce a water-soluble Small molecular weight, unbranched fiber polysaccharides and glycoproteins, uniform chemical composition, can be orally absorbed. [0004] The known small-molecule citrus pectin is used as a health food to regulate gastroint...

Claims

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Application Information

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IPC IPC(8): A61K36/752A61K31/732A61K45/06A61K39/395A61P35/00A61P35/04
CPCA61K31/732A61K36/752A61K39/39558A61K45/06A61P35/00A61P35/04A61K2300/00
Inventor 郭秀丽王磊赵麟金秋阳
Owner SHANDONG UNIV
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