A device and method for measuring optical parameters of in vivo tissue based on hyperspectral images

Abstract

The invention discloses a device and method for measuring optical parameters of in vivo tissue based on hyperspectral images. The device includes: a halogen lamp as a light source, which has continuous wide spectrum characteristics and is suitable for sample measurement in the visible spectrum; a filter wheel group as a spectroscopic The device includes filters with different central wavelengths; the camera is matched with a focusing lens to achieve high-resolution imaging; the two-dimensional image information and diffuse reflectance spectrum of the phantom are captured, and the spectral reconstruction based on the Wiener fitting principle is used to collect images of discrete wavelengths. The structure shortens the acquisition time of tissue imaging and realizes the acquisition of multi-spectral images of different bands. The method includes: obtaining an 8-channel image of the sample through an in-body tissue optical parameter measurement device; inputting the reflectance value of the narrow-band filter channel and the measured diffuse reflectance spectrum value, and using the Wiener matrix to reconstruct the spectrogram; combining the Monte Carlo look-up table method with Modeling and data fitting are performed on the forward model to obtain the simulated reflectance spectrum of the sample; the optical parameters of the tissue and the concentration of the phantom are obtained by using the optimal inversion algorithm.

Description

technical field [0001] The invention relates to the field of in-body tissue optical imaging and detection, in particular to an in-body tissue optical parameter measurement device and method based on hyperspectral images. Background technique [0002] Most biological tissues are composed of proteins, nucleic acids, carbohydrates, lipids, water, vitamins and trace elements. Due to the strong scattering effect of macromolecular substances on photons in the tissue, when the tissue structure and the content of various components in the tissue change, the propagation path of light in the tissue will change accordingly, resulting in the occurrence of optical properties in the tissue. Variety. In biomedical photonics, the interaction between light and biological tissue is mainly in the form of absorption and scattering. Parameters such as absorption coefficient, scattering coefficient, refractive index and anisotropy factor are often used to quantitatively describe the optical prop...

AI Technical Summary

Problems solved by technology

However, the current method for extracting tissue optical parameters based on hyperspectral measurement is limited in terms of spectral imaging speed and requires multiple continuous spectral measurements. The system's spectral method is relatively complicated, and it is difficult to achieve fast imaging

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