49 results about "Tissue optics" patented technology

New devices and methods are provided for noninvasive and noncontact real-time measurements of tissue blood velocity. The invention uses a digital imaging device such as a detector array that allows independent intensity measurements at each pixel to capture images of laser speckle patterns on any surfaces, such as tissue surfaces. The laser speckle is generated by illuminating the surface of interest with an expanded beam from a laser source such as a laser diode or a HeNe laser as long as the detector can detect that particular laser radiation. Digitized speckle images are analyzed using new algorithms for tissue optics and blood optics employing multiple scattering analysis and laser Doppler velocimetry analysis. The resultant two-dimensional images can be displayed on a color monitor and superimposed on images of the tissues.

An energy-based tissue-sealing system and method provide higher sealing quality by measuring and using optical feedback parameters that are directly correlated to structural changes of tissue. The tissue-sealing system includes a sealing energy source, an instrument having a mechanism for grasping and deforming the tissue and for delivering sealing energy to the tissue, a light source, optical sensors, and a controller for controlling parameters of the sealing energy generated by the sealing energy source based upon the optical parameters of the tissue structure sensed by the optical sensors. At the beginning of a sealing procedure, the controller may monitor an initial optical parameter of the tissue and select a target trajectory of tissue optical parameters based on the initial optical parameter. During the sealing procedure, the controller monitors at least one optical parameter of the tissue structure and controls at least one parameter of the sealing energy based on the at least one optical parameter.

An intravenous infiltration detection apparatus for monitoring intravenous failures, which applies an optical method coupled with fiber optics and algorithms for tissue optics to provide a means for noninvasive detection of intravenous infiltration surround the site of IV injection. In the invention, the tissue surrounding the injection site is exposed to a single-wavelength of electromagnetic radiation, and light is collected with only one detector. Changes in the relative intensity of the radiation reflected, scattered, diffused or otherwise emitted provide a means for monitoring infiltration. The invention provides routine, automated, continuous, and real-time monitoring for patients undergoing IV therapy.

Apparatus and methods for spectroscopic analysis of human tissue to classify an individual as diabetic or non-diabetic, or to determine the probability, progression or level of diabetes in an individual. Tissue optical information of an individual, including at least a measurement of at least one wavelength or group of wavelengths indicative of glycosylated collagen content in tissue, is analyzed using multivariate techniques. The multivariate techniques include an algorithm developed from optical information from individuals having a known disease state. At least one factor in the algorithm is dependent on or a function of the measurement of the at least one wavelength or group of wavelengths indicative of glycosylated collagen content in tissue from the optical information of individuals forming the database.

The invention belongs to the field of optical parameter measurement in tissue optics research, and relates to a multi-wavelength tissue optical parameter trans-construction method based on a neural network. The method comprises: using double integrating sphere technology and a high-sensitivity photoelectric detector to collect reflected light and transmitted light on the surface of a tested sample, and using a method of relative measurement to obtain the reflectivity, diffuse transmittance and collimation transmittance of the sample; using a Monte Carlo method to establish a mapping model of a tissue optical parameter (mua, mus and g) to a measurement amount (Rd, Td and Tc); establishing a BP neural network containing two buried layers, and adopting a gradient descent weight learning function to train a BP network; and predicting the optical parameter of the measured sample through the (Rd, Td and Tc) obtained by measurement in real time, wherein an input signal of the neural network is (Rd, Td and Tc), and an output signal is (mua, mus and g). The invention provides a device adopted by the method at the same time. The method provided by the invention can quickly and accurately measure the tissue optical parameters under multi-wavelength.

The invention belongs to the field of optical parameter measurement in tissue optics research, in particular to an optical parameter reconstruction method based on frequency-domain near-infrared photoelasticimetry. The optical parameter reconstruction method comprises the following steps of: positively modeling by utilizing a time-domain Monte Carlo simulated biological tissue model; and extracting frequency-domain information by utilizing a fast formula; acquiring frequency-domain information under any optical parameter by adopting a rapid MC (Multiple-Carrier) module based on a binary polynomial to achieve the aim of accurately reconstructing the optical parameters of tubular tissue by integrating an L-M (Levenberg-Marquardt) optimization algorithm and improve the reconstruction accuracy by introducing a cluster analysis method. The invention has the advantages of simultaneously reconstructing an absorption coefficient and a scattering coefficient of tissue and reconstructing the speed and the accuracy and can be used for accurately predicting the optical parameters of a measured sample in real time by the measured frequency-domain information, i.e. the amplitude and the phase (A, phi).

A quantitative Monte Carlo simulation method for transmission characteristics of light in a biological tissue pertains to the application of spectrum technology; the method firstly describes a spatial structure of the target biological tissue as a three-dimensional digital matrix, the surface characteristics of an incident light source are a set of photons with the set number, the position of the incident light source and the direction of the incident light are given to the initial position and the direction of each photon; the transmission process of each photon is tracked, and then a light absorption matrix and the information of all escaped photons are output. The method quantitatively simulates the light transmission characteristics in any biological tissue with three-dimensional structure by establishing a Monte Carlo model of transmitting the light in the tissue. The method has high precision and good stability, which can obtain the rich characteristics of the light transmission in the real biological tissue. The application of the method can optimize the design of a biological tissue optical detection/imaging system, provide precise information for the dose quantification for light treatment and provide an accurate and reliable tool for the positioning of a detection area and the quantitative analysis of signals in the biological tissue by using the spectrum technology.

Embodiments of the present disclosure provides systems, devices, and methods for non-invasively modifying, maintaining, or controlling local tissue optical properties. Methods and devices of the disclosure may be used for optically clearing tissue, for example, for diagnostic and/or therapeutic purposes. A method of optically clearing a tissue may comprise contacting the tissue with an optical clearing device having a base, an array of pins fixed to one side of the base, a brim fixed to the base, an inlet port in the base, an exit port in the base, and a handpiece interface tab fixed to the side of the base opposite the array of pins, applying a mechanical force to the tissue, and illuminating said tissue with at least one wavelength of light through the optical clearing device. A method may further comprise controlling the temperature of the tissue illuminated.

The invention discloses an analysis method for obtaining stable state/transient state light diffusion characteristic, which belongs to the field of optical detection in biological and medical engineering science. The analysis method of the present invention utilizes easy-obtained optical parameters of each tissue in organism and light source parameters of optical detector, describes matrix of organism tissue structure and Monte Carlo model to calculate diffusion characteristic, employs a light transmission Monte Carlo method which is suitable for multi-voxel biological tissue, can be applied into organism with complicated three dimensional structure, and has higher accuracy and stability. The method sets two parameters of diffusion characteristic sampling frequency and sampling interval, introduces photon movement timing function into a recording module, and has characteristic of intelligently obtaining the stable state/transient state light diffusion characteristic. The invention also discloses a system for obtaining the stable state/transient state light diffusion characteristic, which includes a parameter input module, a tissue model input module, a diffusion characteristic calculation module, a diffusion characteristic recording module and a diffusion characteristic output module.

The invention provides a rapid non-destructive tissue biopsy method and a technique based on a spatial frequency domain-modulated large area resolution microstructure. The method comprises the steps of 1, controlling a light source to output a plurality of spatially modulated light stripe patterns of different frequencies onto a tissue sample; 2, repeatedly collecting the reflective light intensity scattered by the tissue sample by means of a CCD; 3, analyzing and processing the collected data of the reflective light intensity; 4, demodulating the data into an AC component, a DC component and a modulation transfer function through the standard three-phase shift method, the SSMD demodulation method or the like, wherein the AC component and the DC component are different in frequency; 5, obtaining a scattering structure coefficient SSI. Based on the high-spatial-frequency SFDI modulation, not only an absorption coefficient and a scattering coefficient can be obtained, but also the phase function of the scattered light and the scattering structure coefficient SSI can be obtained. The high-spatial-frequency SFDI has important application value in the field of the large-area histological diagnosis. Based on the high-spatial-frequency SFDI, large-area tissue optical properties and the distribution diagram of scattering characteristics can be quantitatively obtained for the objective diagnosis of biological tissues.

A measuring system for estimating skin tissue microvascular depth comprises a biological tissue sample information collection module, a light source module, a speckle imaging module, a precise reflection signal measuring module, a radiation reverse method analysis module and an evaluation and correction module. On the basis of these six modules, skin tissue sample information is collected to acquire a skin tissue model and tissue optical parameters; blood and vessel two-dimensional profile information of a focus microvessel is acquired through a speckle system, and the vascular diameter is acquired through image recognition; a high-accuracy reflection signal of a tissue sample is acquired through reflective signal measuring; the above three kinds of information is taken as input parametersto enter the radiation reverse method analysis module to acquire an estimated value of depth of the focus microvessel, and distribution laws of laser energy in skin tissue is evaluated; recommendation laser thermal therapy parameters are acquired by combining with blood information, and theoretical guidance is provided for improving clinical treatment effect on vascular dermatosis like port-winestain.

The invention discloses a hyperspectral image based in-vivo tissue optical parameter measuring device and method. The device comprises a halogen lamp as a light source, a focusing lens, a light filtering wheel group, a camera and a spectrograph; the halogen lamp has the continuous broad-spectrum characteristic and is suitable for sample measurement in a visible spectrum; the light filtering wheelgroup is used as a light splitting device and comprises optical filters with different center wavelengths; the camera is matched with the focusing lens to realize high-resolution imaging; two-dimensional image information and a diffuse reflection spectrum of a phantom are captured, the acquisition time of the tissue imaging is shortened by collecting images with discrete wavelengths and utilizingWiener fitting principle based spectral reconstruction, and acquisition of multispectral images of different wavebands is realized. The method comprises the following steps of acquiring 8-channel images of a sample through the in-vivo tissue optical parameter measuring device; inputting a reflection value of a narrow-band light filtering channel and a measured diffuse reflection spectrum value, and performing reconstruction with a Wiener matrix to obtain a spectrogram; performing modeling and data fitting by combining a Monte Carlo look-up table method and a forward model to obtain a simulatedreflection spectrum of the sample; and acquiring tissue optical parameters and a phantom concentration by adopting an optimized deconstruction algorithm.

The present invention is directed to a method for real-time characterization of spatially-resolved tissue optical properties using OCT/LCI. Imaging data are acquired, processed, displayed and stored in real-time. The resultant tissue optical properties are then used to determine the diagnostic threshold and to determine the OCT/LCI detection sensitivity and specificity. Color-coded optical property maps are constructed to provide direct visual cues for surgeons to differentiate tumor versus non-tumor tissue. These optical property maps can be overlaid with the structural imaging data and/or Doppler results for efficient data display. Finally, the imaging system can also be integrated with existing systems such as tracking and surgical microscopes. An aiming beam is generally provided for interventional guidance. For intraoperative use, a cap/spacer may also be provided to maintain the working distance of the probe, and also to provide biopsy capabilities. The method is usable for research and clinical diagnosis and/or interventional guidance.

The invention provides an intelligent cerebral function oxyhemoglobin saturation monitoring measurement simulation algorithm. The algorithm comprises the following steps: constructing a measurement simulation space; measuring distribution optical characteristics and spatial distribution, and calculating energy deposition according to the spatial distribution; obtaining an initial overpressure source under a thermal elastic stress constraint condition; when the laser pulse length is less than the thermal relaxation time, the medium is uniform and the sound velocity is constant, obtaining a photo-acoustic velocity wavelength propagation formula; carrying out fitting by adopting a Green function to obtain a positive solution of the photo-acoustic velocity wavelength; modeling a frequency response curve of a transducer as a Gaussian function, and constructing a mapping model between the photo-acoustic velocity with wavelength limitation and the number of detection points to obtain an optical distribution theoretical value model; and solving an optimized value of the minimum value of an error function and a cerebral function oxyhemoglobin saturation monitoring measurement reference standard. According to the invention, an algorithm using the Green function and Monte Carlo radiation flux to simulate the optical parameter distribution of reconstructed tissues is adopted, and an intelligent cerebral function oxyhemoglobin saturation monitoring measurement standard is effectively constructed.

The invention belongs to the technical field of tissue optical measurement and relates to a calibration method for a time domain fluorescence chromatography imaging system. The method is used for calibrating transmission factors of each passage of a system and comprises the following steps that: a calibration device used for replacing an imaging cavity in an imaging system is built; a source optical fiber and a detection optical fiber are respectively inserted onto the imaging cavity; some pieces of lens paper are placed into an insertion groove, and the detection optical fiber is connected with the first passage; a 780nm laser is opened, and the attenuation rate of all neutral filters arranged on a filter wheel corresponding to the passage is calibrated; the 780nm laser is turned off, an830nm laser is turned on, and the transmission rate of 830nm long pass filters arranged on a filter wheel corresponding to the passage is calibrated; and the steps are repeated, and the calibration of the relative attenuation rate of all neutral filters arranged on the filter wheel on each passage and the transmission rate of the 830nm long pass filters is completed. The differences on the systempassage transmission performance can be eliminated.

The invention relates to a fluorescence imaging system for quantitative detection of spatial distribution of a photosensitizer. The fluorescence imaging system is characterized by comprising a first LED light source, a first freeform-surface total reflection lens, a first fly-eye lens, a first integrated lens, a first reflection mirror, a second LED light source, a second freeform-surface total reflection lens, a second fly-eye lens, a second integrated lens, a second reflection mirror, a first semi-transparent semi-reflective mirror, a second semi-transparent semi-reflective mirror, a laser light source, a beam expander, a third fly-eye lens, a digital micromirror device, a projection lens, a condensing lens, a CMOS (complementary metal oxide semiconductor) camera and a computer. The fluorescence imaging system has the advantages that diffuse reflection images are adopted to quantitatively calibrate the effect of tissue optical properties on fluorescence images, quantitative fluorescence imaging of the spatial distribution of the photosensitizer is realized, and the spatial distribution and variation conditions of the concentration of the photosensitizer in diseased tissue are obtained.

A device, system, and method for identifying an area of ablated tissue. Specifically, the device, system, and method may include a device having one or more fiber sensors and a processing unit for receiving emitted and/or reflected light from tissue and making one or more determinations based on the received light regarding ablated tissue, tissue contact, and/or occlusion. Each of the fiber sensors may include at least one multimode waveguide segment and at least one singlemode waveguide segment, or each of the fiber sensors may be singlemode waveguide. Further, each fiber sensor may have a core with long-period grating for optically coupling light from tissue to the fiber sensor. The processing unit may include a beam processing apparatus and a hyperspectral imaging and analysis apparatus, and may optionally include a light source.

The invention discloses a method and a device for measuring optical parameters of red jujube tissues by polarized scattering, relate to the field of methods for measuring optical parameters of agricultural product quality, and solve the problems that the influence of surface thin-layer textures and refractive index distribution characteristics on the spectral polarization state is neglected in the non-destructive detection of red jujube quality, so that high detection accuracy cannot be obtained. The method mainly measures the optical parameters of red jujube tissues by polarized scattering,and comprises the steps: performing theoretical simulation on an equivalent scattering model of different structural characteristics of red jujube to obtain theoretical control characteristic parameters; measuring physical and chemical indicators of red jujube slices in different periods, and marking a corresponding red jujubepolarized scattering diagram to obtain a correspondence between opticaltissue parameters and micropore scale characteristic parameters; performing multi-angle polarized reflection and polarized transmission detection on the slices to obtain actual optical tissue parameters; and correcting the deviation between the theoretical control parameters and the actual optical tissue parametersto obtain an optical parameter model of the red jujube tissues for judging the quality of red jujube in actual test.

Disclosed are compositions, methods, and kits for clearing tissue that preserve cellular morphology, reporter fluorescence, and epitope labeling which allow for quantitative phenotypic analysis of intact organs. The compositions include, for example, a compound of formula R¹—C(X)—NR²R³, wherein R¹ is alkyl, haloalkyl, hydroxyalkyl, amino, or alkylamino, X is O or S, and R² and R³ are independently H, alkyl, or hydroxyalkyl, a salt thereof, or a combination thereof, and at least one non-ionic density gradient medium. Also disclosed are methods for clearing tissue comprising positioning a tissue in a tissue clearing composition and allowing a tissue clearing composition to permeate the tissue. Further disclosed are methods for visualizing tissue characteristics which involve fixing a tissue, staining the tissue, positioning the tissue in the tissue clearing composition and allowing the tissue clearing composition to permeate the tissue, and imaging the tissue utilizing a microscope or tissue scanning device.

The invention discloses a preparation method of a second near-infrared window nano photosensitizer based on NDI molecules. The method comprises the following steps: A, reacting 1,4,5,8-naphthalene tetracarboxylic anhydride with dibromoisocyanuric acid under the catalysis of fuming sulfuric acid to generate tetrabromo-substituted naphthalene tetracarboxylic anhydride; B, performing an alkylation reaction on the tetrabromo-substituted naphthalene tetracarboxylic anhydride and dodecylamine, and performing cyclization with 1,5-diaminonaphthalene to obtain an NDI photosensitizer; and C, dissolvingthe NDI photosensitizer prepared in the step A into tetrahydrofuran, then slowly dropwise adding into rapidly stirred pure water, carrying out rotary evaporation on tetrahydrofuran, and then centrifuging to obtain the multifunctional NDI nano photosensitizer. According to the invention, the defects in the prior art can be overcome, the biological tissue penetrating power can be effectively enhanced, the fluorescence imaging signal-to-noise ratio is reduced, and high-resolution and high-contrast oral squamous cell carcinoma tissue optical imaging real-time monitoring is achieved.

The invention discloses a quick biological tissue optical characteristic parameter measurement method based on short-pulse laser reflection signal peak inversion and belongs to the technical field of biological tissue optical characteristic parameter measurement. The problem that the response time for the conventional biological tissue optical characteristic parameter measurement is long is solved. The method comprises the steps of measuring a peak value Rmax of a time domain semispherical reflection signal on the surface of a sample block to be measured, changing a pulse width tp of a laser device to obtain n groups of semispherical reflection signal peak values Rmax,i according to the former measuring method, setting the scattering albedo Omega of a sample to be measured and an asymmetric factor g of the sample to be measured, and obtaining undetermined coefficients a and b through an experience formula; obtaining estimated values Rmax,i of the n groups of semispherical reflection signal peak values, and performing least-square on estimated values Rmax,i of time domain semispherical reflection signal peak values and time domain semispherical reflection signals Rmax,i corresponding to the estimated values Rmax,i of the signal peak values to obtain a difference value; judging whether the least-square difference value is smaller than a threshold value Epsilon, if the least-square difference value is smaller than the threshold value Epsilon, implementing quick measurement on the biological tissue optical characteristic parameter based on the short-pulse laser reflection signal peak inversion, otherwise, continuing performing measurement. The quick biological tissue optical characteristic parameter measurement method is suitable for biological tissue optical characteristic parameter measurement.