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Application of sanguinarine in inhibiting and removing multi-drug-resistant serratia marcescens biofilm

A technology of Serratia organisms and Serratia marcescens, which is applied in the field of medical applications, can solve the problems such as the inhibition and scavenging effects of sanguinarine that have not been reported in the literature, achieves wide application value, solves infection problems, and reduces mortality. Effect

Inactive Publication Date: 2020-04-07
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, no literature has reported the inhibitory and clearing effects of sanguinarine on the biofilm of multidrug-resistant Serratia marcescens

Method used

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  • Application of sanguinarine in inhibiting and removing multi-drug-resistant serratia marcescens biofilm
  • Application of sanguinarine in inhibiting and removing multi-drug-resistant serratia marcescens biofilm
  • Application of sanguinarine in inhibiting and removing multi-drug-resistant serratia marcescens biofilm

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Drug susceptibility test of Serratia marcescens;

[0031] Taking 5 strains of Serratia marcescens as starting strains, AMP, SAM, CFZ, CTT, CRO, CAZ, TOB, TZP, CIP, LEV, AMK, SXT, FEP, ETP, IPM, F, ATM, CN and other 18 strains were selected. A test of commonly used antibiotics.

[0032] Pick and culture the pure colonies for 24 hours and dissolve them evenly in 5mL TSB, and adjust their turbidity to be equal to that of a 0.5 McFarland turbidimetric tube. Antibiotics, bacterial liquid and TSB liquid medium were added to 96-well culture plates by double dilution method for overnight culture, and the minimum inhibitory concentration (MIC) of antibiotics against Serratia marcescens was determined by microplate reader. There are three parallel groups of liquid medicines with different concentrations to ensure the reliability of the experimental data. The bacteriostasis results were judged according to the standards of the American Clinical Laboratory National Standardizatio...

Embodiment 2

[0036] Inhibitory effect of sanguinarine on biofilm of multi-drug resistant strains;

[0037] In the present invention, a single active ingredient sanguinarine is used as the research object, and a standard bacterial strain is used as a control to conduct research on the inhibition of biofilm. The pure colonies picked and cultured for 24 hours were evenly dissolved in 5 mL of TSB liquid medium, and the OD600 value was determined to be 0.5 by a microplate reader. Sanguinarine with a concentration of 500 mg / mL was prepared with dimethyl sulfoxide as a drug solution, and sterile glass slides were added to a 24-well plate, and the drug solution, bacterial solution and TSB liquid medium were added to 24 Cultivate overnight in a well culture plate, so that the final concentration of the drug solution is 125 mg / mL, 62.5 mg / mL, 31.25 mg / mL, 15.63 mg / mL, 15.61 mg / mL, 3.90 mg / mL. Afterwards, wash three times with 0.1M PBS, add 200 µL crystal violet dye for 20 min, wash away excess dye,...

Embodiment 3

[0039] Scavenging effect of sanguinarine on mature biofilm of multi-drug resistant strains

[0040] The invention takes sanguinarine, a single active ingredient, as the research object, and uses standard bacterial strains as a control to carry out research on the removal of mature biofilms. Pick the pure colonies cultured for 24 hours and dissolve them evenly in 5mL TSB liquid medium, adjust its turbidity to 0.5 McFarland turbidity, and measure its OD600 value with a microplate reader. Prepare sanguinarine with a concentration of 1000 mg / mL in dimethyl sulfoxide as a drug solution, add sterile glass slides and 1 mL of bacterial solution to a 24-well plate and incubate for 24 h to form a mature film, and then add drug to each well. The solution was cultured for 4 h, so that the final concentration of the drug solution was 62.5 mg / mL, 125 mg / mL, 250 mg / mL, 500 mg / mL, 1000 mg / mL. Afterwards, wash three times with 0.1M PBS, add 200 µL crystal violet dye for 20 min, wash away exce...

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Abstract

The invention discloses application of a compound sanguinarine in inhibition and removal of a multi-drug-resistant serratia marcescens biofilm. According to the fact that sanguinarine has good biofilminhibiting and removing effects on serratia marcescens, formation of a serratia marcescens biofilm can be inhibited, and the lowest inhibition concentration of the biofilm is 15.6 micrograms / mL. In addition, sanguinarine can effectively remove a mature serratia marcescens biofilm, and the lowest removal concentration of the biofilm is 250 micrograms / mL. According to the invention, the natural product sanguinarine is adopted to inhibit and remove the multi-drug-resistant serratia marcescens biofilm, so that the infection treatment difficulty caused by the serratia marcescens biofilm can be effectively relieve or solved, the cure rate is improved, and a new lead compound is provided for inhibiting the formation of serratia marcescens biofilms and removing mature biofilms. The invention hasimportant practical significance.

Description

technical field [0001] The invention belongs to the technical field of medical application, and in particular relates to the application of sanguinarine in inhibiting and clearing multidrug-resistant Serratia marcescens biofilm. Background technique [0002] Serratia marcescens is a Gram-negative bacillus belonging to the family Enterobacteriaceae. It is an opportunistic pathogen that is widely distributed in hospital environments and can cause nosocomial infections. separated to. In recent years, with the widespread application of antibacterial drugs, the detection rate and drug resistance rate of the bacteria have been increasing, and it has become one of the main pathogens of hospital infection. Biofilm is a kind of bacterial aggregation film, which is formed by bacteria attaching to the surface of an object and secreting a large number of exopolysaccharide-protein complexes to wrap themselves. After the biofilm is formed, the bacteria are wrapped in the biofilm, which ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4741A61P31/04
CPCA61K31/4741A61P31/04
Inventor 钱卫东付玉婷刘淼杨敏王文静李康帆何维维高悦
Owner SHAANXI UNIV OF SCI & TECH
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