Preparation method and application of gold nano colloid type time temperature indicator
A technology of time temperature and indicators, applied in the field of detection, to achieve the effects of obvious color changes, easy preparation, and irreversible color changes
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Embodiment 1
[0023] (1) Configuration of gelatin solution: Take 116.667g of gelatin, add 1L of pure water, stir at 90°C at a speed of 500r / min for 30 minutes to completely dissolve the gelatin, and then obtain a gelatin solution of 116.67g / L;
[0024] (2) Configuration of chloroauric acid tetrahydrate solution: Take 1.458 g of chloroauric acid tetrahydrate, add 1 L of pure water, and shake well to obtain a solution of chloroauric acid tetrahydrate with a concentration of 1.458 g / L.
[0025] (3) Preparation of gold nanocolloid time-temperature indicator: 50 mL of chloroauric acid tetrahydrate solution was slowly poured into 50 mL of gelatin solution, and the mixture was heated and stirred at 90° C. at a speed of 500 r / min for 10 minutes. Take 1.2mL of the above mixed solution and place it in a 1.5mL disposable micro plastic cuvette to obtain a time and temperature indicator.
[0026] According to the above production method, three identical nano-type time and temperature indicators were pro...
Embodiment 2
[0036] The gold nano-colloid time-temperature indicator prepared in Example 1 is used to indicate the product within 25KJ / mol of its own activation energy difference. The nano-type time and temperature indicator prepared in Example 1 can be used to indicate products that have deteriorated due to lipid oxidation or nutrient loss, such as pork, mutton, butter, etc.
[0037] When the absorbance value of the time-temperature indicator no longer changes, the indicator reaches the end of discoloration, and the time corresponding to the end of discoloration is the color life of the indicator. The color lifetimes of gold nanocolloid time-temperature indicators with different preparation parameters are shown in Table 2.
[0038] Table 2 Color lifetime of gold nanocolloid time temperature indicator with different preparation parameters
[0039]
[0040]
[0041] By fitting the relationship between the gold nanocolloid time-temperature indicator color lifetime and its preparation ...
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