A top-immersion microsphere lens group capable of realizing super-resolution imaging in a large field of view in different environmental media

Abstract

The invention discloses an upper-immersion microsphere lens group capable of realizing super-resolution imaging in a large field of view in different environmental media, including high-refractive-index transparent microspheres immersed in a low-refractive-index transparent medium on the transparent medium. An upper superimposed microlens is provided, and the upper superimposed microlens is provided with a bottom plane, and the optical axis of the transparent microsphere is consistent with the upper superimposed microlens; the lower surface of the transparent microsphere can be truncated into a solid immersion mirror, In this way, the focus of the upper immersion microsphere lens group is close to the interface of the truncated part, and when super-resolution images are realized, the image distortion is small, and the more truncated parts, the smaller the image distortion. The upper immersion microsphere lens group of the present invention is mainly used in a system and method for realizing super-resolution optical microscopic imaging under a large field of view.

Description

technical field [0001] The invention relates to an optical imaging system and method, in particular to a method and system for realizing super-resolution optical microscopic imaging under a large field of view by an upper-immersion microsphere lens group. Background technique [0002] At the end of the 19th century, the German scientist Ernst Abbe defined the resolution of the optical microscope, thinking that the resolution limit of the optical microscope is half of the wavelength of light, that is, about 0.2 microns, which is the famous optical diffraction limit. According to the Rayleigh criterion, the resolution of an optical microscope can be determined by the following formula: r=0.61λ / NA; λ is the wavelength of the light wave; NA is the numerical aperture of the objective lens. For most of the 20th century, scientists believed that optical microscopy would never break beyond the diffraction limit. To achieve higher resolution, scientists have had to rely on more comp...

AI Technical Summary

Problems solved by technology

This method uses larger-sized microspheres to increase the field of view, but it must be used in liquid or solid media, which is very inconvenient, and this method can only be imaged at a fixed position, and does not have the ability to image at any position and in a large range

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