Docked aptamer eab biosensors

A body-sensing and complex technology, applied in the direction of biomaterial analysis, biochemical equipment and methods, instruments, etc., can solve the problems of inability to produce aptamers, time-consuming, etc.
CN110998326AInactive Publication Date: 2020-04-10ECCRINE SYST
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ECCRINE SYST
Publication Date
2020-04-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention describes electrochemical aptamer-based biosensing devices and methods that are configured to produce a detectible signal upon target analyte interaction, with reduced reliance on a conformational change by the aptamer. The disclosure includes docked aptamer EAB sensors for measuring the presence of a target analyte in a biofluid sample. The sensors include an electrode capable of sensing redox events, and aptamer sensing elements with aptamers selected to interact with a target analyte. Each aptamer sensing element includes a molecular docking structure attached to the electrode, and an analyte capture complex that includes an aptamer releasably bound to the docking structure, and an electroactive redox moiety. Upon aptamer binding with a target analyte, the analyte capturecomplex separates from the docking structure. The separation of the analyte capture complex from the docking structure produces a positional change in the redox moiety that is detectable by the sensing device on interrogation of the electrode.
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Description

[0001] Cross References to Related Applications

[0002] This application claims priority to U.S. Provisional Application No. 62 / 523,835, filed June 23, 2017, and the specification of this application is based on PCT / US17 / 233399, filed March 21, 2017, the disclosures of which are incorporated by reference Incorporated into this article as a whole. Background technique

[0003] Although perspiration (sweat) has many ergonomic advantages compared to other biofluids (especially in "wearable" devices), compared with established biofluids (blood, urine, and saliva), Sweat remains an underutilized source of biomarker analytes. When compared more closely with other non-invasive biofluids, its advantages extend even beyond ergonomics: sweat can provide superior analyte information. Historically, a number of difficulties have kept sweat from gaining a place among the clinical biofluids of choice. These challenges include very small sample volumes (nL to μL), unknown concentrations d...

Claims

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