Docked aptamer eab biosensors

A body-sensing and complex technology, applied in the direction of biomaterial analysis, biochemical equipment and methods, instruments, etc., can solve the problems of inability to produce aptamers, time-consuming, etc.

Inactive Publication Date: 2020-04-10
ECCRINE SYST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Not only is this process time consuming, it may ultimately not yield suitable aptamers

Method used

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  • Docked aptamer eab biosensors
  • Docked aptamer eab biosensors
  • Docked aptamer eab biosensors

Examples

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Embodiment Construction

[0035] The embodiments described herein address the shortcomings of the SELEX process in biofluid sensor development by using docking aptamer EAB sensors. The disclosed sensors reduce the requirement to identify aptamers that not only preferentially bind the target analyte, but also exhibit a conformational change sufficient to generate an analyte capture signal that is distinct from an unbound signal. Specifically, docked aptamer sensors detect analyte capture using redox moiety position changes caused by the dissociation of bound aptamers from the docking structure. As described herein, a docking aptamer EAB sensor includes an aptamer initially bound to a docking structure. When bound to the docking structure, the redox moiety associated with the aptamer produces a first redox signal that can be measured by the sensing device. After interacting with the analyte, the aptamer changes shape to bind the analyte, causing the aptamer to disengage from the docked structure. Separ...

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Abstract

The invention describes electrochemical aptamer-based biosensing devices and methods that are configured to produce a detectible signal upon target analyte interaction, with reduced reliance on a conformational change by the aptamer. The disclosure includes docked aptamer EAB sensors for measuring the presence of a target analyte in a biofluid sample. The sensors include an electrode capable of sensing redox events, and aptamer sensing elements with aptamers selected to interact with a target analyte. Each aptamer sensing element includes a molecular docking structure attached to the electrode, and an analyte capture complex that includes an aptamer releasably bound to the docking structure, and an electroactive redox moiety. Upon aptamer binding with a target analyte, the analyte capturecomplex separates from the docking structure. The separation of the analyte capture complex from the docking structure produces a positional change in the redox moiety that is detectable by the sensing device on interrogation of the electrode.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to U.S. Provisional Application No. 62 / 523,835, filed June 23, 2017, and the specification of this application is based on PCT / US17 / 233399, filed March 21, 2017, the disclosures of which are incorporated by reference Incorporated into this article as a whole. Background technique [0003] Although perspiration (sweat) has many ergonomic advantages compared to other biofluids (especially in "wearable" devices), compared with established biofluids (blood, urine, and saliva), Sweat remains an underutilized source of biomarker analytes. When compared more closely with other non-invasive biofluids, its advantages extend even beyond ergonomics: sweat can provide superior analyte information. Historically, a number of difficulties have kept sweat from gaining a place among the clinical biofluids of choice. These challenges include very small sample volumes (nL to μL), unknown concentrations d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543
CPCC12N2310/16G01N33/54386G01N2458/30G01N27/3277G01N33/5308G01N33/5438
Inventor 罗伯特·比奇米克尔·拉森雅各布·A·伯特兰加维·格特鲁普
Owner ECCRINE SYST
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