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A bacterial sensor chip for rapid enrichment and in situ detection of pathogenic bacteria

A sensor chip, in-situ detection technology, applied in laboratory containers, fluid controllers, material electrochemical variables, etc., can solve the problems of long detection time, low pathogenic bacteria enrichment efficiency, etc. properties, good ability to capture pathogenic bacteria, and the effect of improving efficiency

Active Publication Date: 2022-01-07
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a bacterial sensor chip for rapid enrichment and detection of pathogenic bacteria in environmental space, based on filtration enrichment, microfluidic The principle of control and electrical impedance sensing, combined with electrode surface material modification technology, laser etching and digital engraving processing technology, realizes efficient, in-situ detection and identification of pathogenic bacteria in environmental space

Method used

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  • A bacterial sensor chip for rapid enrichment and in situ detection of pathogenic bacteria
  • A bacterial sensor chip for rapid enrichment and in situ detection of pathogenic bacteria
  • A bacterial sensor chip for rapid enrichment and in situ detection of pathogenic bacteria

Examples

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Effect test

Embodiment 1

[0037] Example 1 Microporous copper mesh electrode surface modification

[0038] 0.5% chitosan aqueous solution was used as electrolyte. The copper mesh (aperture: 200 mesh, size: 10×10mm) was used as the working electrode, the platinum sheet electrode was used as the auxiliary electrode, and Ag / AgCl was used as the reference electrode to modify the surface of the copper mesh. Apply a voltage of -0.9 V, deposit for 20 min, take out the copper grid, deionize and rinse to obtain a Chi hydrogel-modified copper grid, which is cut into 7×7mm. For integration with the designed electrical impedance sensor. Microscopic observation of bare copper mesh and chitosan-modified copper mesh revealed that chitosan was successfully modified to the surface of copper mesh.

Embodiment 2

[0039] Example 2 Fabrication of Bacterial Sensor Chip

[0040] The bacterial sensor chip is a three-layer sandwich structure, including: PMMA shell, microporous copper mesh electrode and L-shaped fluid channel.

[0041] The upper shell 1 is a PMMA plate of 4×4 cm, with a circular hole in the center for gas / liquid inlet a, and the diameter of the circular hole is 8mm. There is a concentric annular groove c on the lower surface of the upper shell for fixing the sealing gasket 5 . The inner diameter of the ring groove is 10mm, the outer diameter is 19mm, and the depth is 1.5mm. There is a 1mm wide post between the circular hole and the concentric ring groove to fix the copper mesh.

[0042] The microporous copper mesh electrode 3 is a disc with a diameter of 8 mm and a pore size of 75 μm, modified with chitosan hydrogel, as in Example 1.

[0043] The lower shell 2 is a 4.6×4.6 cm PMMA plate, and the center of the upper surface is engraved with a circular groove d with a diamet...

Embodiment 3

[0047] Example 3 Capture and detection of Staphylococcus aureus in bacteria-containing aerosol

[0048] The bacterial sensor chips constructed in Example 1 and Example 2 were tested for application. Synthetic Staphylococcus aureus aerosols were prepared using an ultrasonic nebulizer. Put the bacterial sensor chip into the airtight box of the synthetic Staphylococcus aureus aerosol, the outlet of the bacterial sensor chip is connected to the C30L-43H miniature vacuum pump through a PVC hose, turn on the vacuum pump, and realize the filtration and enrichment of Staphylococcus aureus under negative pressure for 5 minutes . After the sampling is completed, inject ultrapure water into the fluid channel, connect the microporous mesh electrode of the bacterial sensor chip to the working electrode of the electrochemical workstation through wires, and connect the flat counter electrode to the counter electrode and reference electrode of the electrochemical workstation , inject the el...

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Abstract

The invention provides a bacteria sensor chip for rapid enrichment and in-situ detection of pathogenic bacteria in environmental space. The bacterial sensor chip involved includes an upper housing with an air inlet / liquid port, a microporous mesh electrode, a lower housing with an air / liquid outlet and a flat counter electrode, an L-shaped fluid channel, and the upper and lower housings pass through the gasket It is sealed to realize the rapid filtration enrichment and in-situ detection of pathogenic bacteria in the environmental space. The microporous mesh electrode, the core component of the bacterial sensor chip, is a mesh metal structure with breathable performance. The surface is modified and modified by composite hydrogel to control the size of the micropores, which can realize the pathogenic bacteria in the environmental space. High-efficiency adsorption, while realizing in-situ electrical detection. The bacterial sensing chip involved has the advantages of no need for sample pretreatment, no need for labeling, quickness, and simplicity, and is suitable for rapid enrichment and in-situ detection of pathogenic bacteria in environmental spaces.

Description

technical field [0001] The invention belongs to the fields of environmental monitoring and biochemical sensing, and in particular relates to a bacterial sensing chip. Background technique [0002] There are many kinds of microorganisms in the environmental space, including bacteria, fungi and viruses, which are easily adsorbed on the surface of air particles and can enter the human lungs, causing serious harm to human health. The content of pathogenic bacteria in the air is one of the important indicators of ambient air monitoring, especially for special environmental spaces such as hospitals, livestock farms, and farms. Therefore, the study of pathogenic bacteria in the air is of great significance to the study and control of environmental safety and the spread of respiratory diseases. At present, the detection of pathogenic bacteria in the air mainly refers to the current standard GB / T 18204.3-2013. The impact method or natural sedimentation method is used to collect the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00G01N27/26
CPCB01L3/502761B01L3/502753G01N27/26B01L2400/0487
Inventor 徐溢苏喜庞义全陈李李顺波王力
Owner CHONGQING UNIV