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Fingerprint spectrum detection method of traditional Chinese medicine radix ophiopogonis
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A technology of fingerprint spectrum and detection method, which is applied in the detection field of traditional Chinese medicine, and can solve the problems of fingerprint spectrum research without multi-index components of Ophiopogon japonicus.
Active Publication Date: 2020-04-17
常熟雷允上制药有限公司
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[0003] At present, the determination of total saponins, total flavonoids, and total polysaccharides of Ophiopogon japonicus is usually determined
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Embodiment 1
[0091] The screening of embodiment 1 chromatographic conditions
[0092] 1. Screening of mobile phase types
[0093] Due to the complexity of the active components of Ophiopogon japonicus, the present invention screened mobile phases such as methanol-water, acetonitrile-water, acetonitrile-0.01% glacial acetic acid water, and acetonitrile-methanol system through a large number of experiments, and due to the presence of saponins and flavonoids in Ophiopogon japonicus Therefore, different mobile phases have different separation efficiencies, and the acidic structure of phenolic hydroxyl groups of flavonoids, adding glacial acetic acid to the mobile phase is conducive to improving the peak shape, so a certain amount of acetic acid is added to the mobile phase system, and finally by comparing the different The mobile phase shows the number of peaks, resolution and peak shape, etc., such as figure 1 , the chromatograms eluted by different mobile phases have large differences. The...
Embodiment 2
[0102] 1, the fingerprint detection method of Chinese medicine Ophiopogon japonicus, it comprises the following steps:
[0103] (1) Preparation of Ophiopogon japonicus test solution:
[0104] Take 8 batches of Ophiopogon japonicus, crush them separately, pass through No. 3 sieve in turn, take 1g precisely, add 20ml of methanol, soak overnight, treat with ultrasonic for 20min, filter, add 20ml of methanol to the filter residue, treat with ultrasonic for 20min, filter, combine twice The filtrate was evaporated to dryness, and the residue was dissolved by adding 2ml of water and slightly heated, added to the activated Agilent C18 column, eluted with 8ml of water, discarded the water, followed by elution with 8ml of 20% methanol, and discarded the eluent , and finally eluted with 3ml of methanol, collected the eluate, evaporated to dryness, dissolved in methanol to a volume of 1ml, and filtered through a 0.22μm microporous membrane to obtain the product;
[0105] (2) Preparation ...
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Abstract
The invention discloses a fingerprint spectrum detection method of traditional Chinese medicine radix ophiopogonis. The method comprises the steps of preparation of a test solution, preparation of a radix ophiopogonis reference medicinal material solution, preparation of a reference substance, establishment of a reference fingerprint spectrum and determination of the fingerprint spectrum of the test solution. According to the structural property characteristic that active ingredients contained in the traditional Chinese medicine radix ophiopogonis comprise saponins, flavonoids and polysaccharide compounds, optimal analysis conditions such as mobile phase composition, gradient elution procedure, flow velocity, detection wavelength, chromatographic column, column temperature and the like arescreened out through a large number of experiments. Multiple experimental verifications show that the fingerprint spectrum detection method of the traditional Chinese medicine radix ophiopogonis cancomprehensively, objectively and accurately detect and evaluate the quality of the radix ophiopogonis, and has important significance for guaranteeing clinical curative effects.
Description
technical field [0001] The invention relates to a detection method of a traditional Chinese medicine, in particular to a detection method of a fingerprint of Radix Ophiopogon japonicus. Background technique [0002] Ophiopogon japonicus (Thunb.) Ker-Gawl is the dried tuber root of Liliaceae Ophiopogon japonicus (Thunb.) Ker-Gawl. It is a commonly used traditional Chinese medicine for nourishing yin. It was first recorded in "Shen Nong's Materia Medica". The efficacy of this medicine is mainly used to treat diseases such as dryness of the lungs, dry cough, and thirst for fluid wounds. Its main components are steroidal saponins, polysaccharides, high isoflavones, amino acids and lipids, etc. Among them, Ophiopogon japonicus saponins, flavonoids and polysaccharides are the main medicinal active ingredients of Ophiopogon japonicus, which have anti-myocardial ischemia, anti-hypoxia, immune activity, Pharmacological effects such as lowering blood sugar, relieving asthma and anti-...
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