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Liquid changing device for multi-cell co-culture simulated weightlessness experiment

A technique for simulating weightlessness and cell culture, applied in tissue cell/virus culture devices, biochemical instruments, biochemical equipment and methods, etc.

Pending Publication Date: 2020-04-21
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the deficiencies in the prior art, the object of the present invention is to provide a liquid exchange device for multi-cell co-culture simulated weightlessness experiments, which solves the two problems of liquid exchange in normal gravity and simulated microgravity co-culture

Method used

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  • Liquid changing device for multi-cell co-culture simulated weightlessness experiment
  • Liquid changing device for multi-cell co-culture simulated weightlessness experiment
  • Liquid changing device for multi-cell co-culture simulated weightlessness experiment

Examples

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Embodiment 1

[0034] Such as figure 1 and Figure 4 As shown, this embodiment discloses a liquid exchange device for a multi-cell co-culture simulated weightlessness experiment, the liquid exchange device includes a gear 401, a gear rod 402, a spring pull wire 403, a spring 404, a piston 405 and two liquid exchange devices driven The second transmission mechanism 9 that moves synchronously. The gear rod 402 is supported on the cell culture device 3 that needs to be exchanged, specifically: the two ends of the gear rod 402 are anchored on the cylinder cover 303 of the cell culture device 3 in the above embodiment through the anchor sleeve 406 . The gear 401 is connected with the gear rod 402, the piston 405 and the spring 404 are arranged in the first cavity 306, one end of the spring pull wire 403 is wound on the gear rod 402, and the other end is connected with the piston 405 through the spring 404, the piston 405 can move along the inner tube 302 slides axially without any clearance, an...

Embodiment 2

[0040] This embodiment discloses a cell culture device, such as image 3 and Figure 5 As shown, it includes an outer cylinder 301, an inner cylinder 302, a cylinder cover 303, a partition plate 304, and a glass slide slot arranged on the partition plate 304 for clamping a cell slide 305; one end of the outer cylinder 301 is closed, and the other end Open; one end of the inner cylinder 302 is open, the other end is sealed with the cylinder cover 303, and the diameter of the cylinder cover 303 is larger than that of the outer cylinder 301; the inner cylinder 302 is sleeved inside the outer cylinder 301, and the inner cavity of the inner cylinder 302 forms a first cavity Cavity 306, the cavity between the inner cylinder 302 and the outer cylinder 301 forms a second cavity 307, the length of the inner cylinder 302 along its axial direction is smaller than the length of the outer cylinder 301 along its axial direction, so that the distance between the outer cylinder 301 and the in...

Embodiment 3

[0043] Such as figure 1 As shown, this embodiment discloses a liquid-changing multi-cell co-culture simulated weightlessness experimental device, which includes a rotary simulated weightlessness unit 1 capable of rotating around a horizontal axis, a normal gravity unit 2 rotating around a vertical axis, and a cell The incubator 3, the liquid changing device 4, and the first power unit 5 that drives the rotary simulated weightlessness unit 1 and the normal gravity unit 2 to rotate; wherein, the rotary simulated weightlessness unit 1 produces a simulated weightlessness effect, and the normal gravity unit 2 does not change the gravity on the cells In this case, the first power unit 5 is a motor.

[0044] Both the rotary simulated weightlessness unit 1 and the normal gravity unit 2 are provided with a cell culture device 3 , specifically, the cell culture device 3 is pluggably installed in the rotary simulated weightlessness unit 1 and the normal gravity unit 2 . The cell culture...

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Abstract

The invention discloses a liquid changing device for multi-cell co-culture simulation weightlessness experiment. The liquid changing device comprises a gear, a gear rod, a spring pull wire, a spring,a piston, a second transmission mechanism and a second power unit; wherein the second transmission mechanism is used for driving the pistons in two liquid changing devices to move synchronously; the second power unit is used for providing power for rotation of the second transmission mechanism and the gear; the gear is connected with the gear rod; the pistons are arranged in an inner cavity of a cell culture device needing liquid replacement; one end of the spring is pressed against the piston, and the other end of the spring is fixed in the inner cavity of the cell culture device needing liquid replacement; one end of the spring pull wire is wound on the gear rod, and the other end is connected with the piston after passing through the spring. The problem of circulation of a culture solution and cell secreta during co-culture of cells growing in different environments and the problem that rotary simulated weightlessness is difficult to carry out for a long time due to nutrient consumption of a culture medium and accumulation of cell excretion wastes in a rotary simulated weightlessness experiment are solved.

Description

technical field [0001] The invention belongs to the field of biomechanical experiment equipment, in particular to a liquid exchange device for multi-cell co-cultivation simulated weightlessness experiment. Background technique [0002] The microgravity environment generated during spaceflight will cause a series of changes in the human cardiovascular system and skeletal muscle system, which will seriously endanger the health of astronauts. Therefore, it is of great significance to study the characteristics and laws of human physiological changes under microgravity, especially to deeply study the mechanism of its occurrence at the cellular and molecular levels. In view of spaceflight opportunities and cost constraints, gyrators are widely used at home and abroad to simulate microgravity effects at the cell level on the ground. On the gyrator, the biological sample remains in the gravitational field, subject to a constant gravitational vector. However, since the gyrator rota...

Claims

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Application Information

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IPC IPC(8): C12M3/00
CPCC12M23/34C12M29/18
Inventor 胡泽兵张舒曹新生石菲
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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