Treatment liquid for use in desorption of steroid included in cyclodextrin
A technology of steroids and treatment liquids, applied in the field of treatment liquids, which can solve the problems of signal intensity drop, inability to exclude batch differences, infection risks, etc., and achieve high-precision results
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1-1
[0089] [Example 1-1] Cyclodextrin to the inspection of inclusion effect of aldosterone
[0090] In this example, aldosterone was used as the antigen, and a buffer-based standard solution was used as the standard solution. Specifically, firstly, cyclodextrins (α-cyclodextrin (αCD), β-cyclodextrin (βCD), hydroxypropyl β-cyclodextrin (HPβCD), hydroxypropyl β-cyclodextrin (HPβCD), Aldosterone was added to a diluted solution of γ-cyclodextrin (γCD) to prepare an aldosterone solution (standard solution) at about 500 pg / mL. The matrix composition of the above dilution was set as "50mM MOPS (pH7.2), 150mM NaCl, 0.1%NaN 3 ".
[0091] For each aldosterone solution, anti-aldosterone antibody-conjugated particles (anti-aldosterone antibody-conjugated magnetic particles (antibody-conjugated particles) The assay was carried out by a two-step method of phosphatase-labeled anti-aldosterone antibody (enzyme-labeled antibody). Specifically, it follows the steps (a) to (f) below.
[0092] (...
Embodiment 1-2
[0099] [Example 1-2] Inspection of the Stabilizing Effect of HPβCD on Aldosterone
[0100] In this example, aldosterone was used as the antigen, and the standard solution of the buffer matrix was used as the standard solution. Specifically, aldosterone was first added to a diluted solution to which HPβCD was added at a concentration of 0.1% or 1%, to prepare an approximately 300 pg / mL aldosterone solution (standard solution). The matrix composition of the above dilution is set as "100mM HEPES (pH7.5), 150mM NaCl, 0.5%BSA, 0.1%NaN 3 ".
[0101]The obtained aldosterone solutions (approximately 300 pg / mL aldosterone solution) were stored at -80°C, 4°C, 25°C, and 37°C for 45 days, respectively, and then used for measurement. For each preserved aldosterone solution, anti-aldosterone antibody-conjugated particles (anti-aldosterone antibody-conjugated magnetic particles (antibody-conjugated particles) prepared by a conventional method) and alkaline phosphatase-labeled anti-aldoster...
Embodiment 1-3
[0109] [Example 1-3] Effect of decomplexing aldosterone from HPβCD-1
[0110] In this example, aldosterone was used as the antigen, and a buffer-based standard solution was used as the standard solution. Specifically, first, add aldosterone to the following standard solution dilution (with HPβCD), prepare about 1600pg / mL aldosterone solution (1600pg / mL CD (+) standard solution), and use the following defatted serum as a mock test Aldosterone was added to a sample diluent to prepare an approximately 1600 pg / mL aldosterone solution (1600 pg / mL sample (simulated test sample)). As the above standard solution dilution (with HPβCD), use "50mM MOPS (pH7.2), 150mM NaCl, 1%HPβCD, 0.1%NaN 3 ", as the above-mentioned defatted serum, "DDC Mass Spect Gold (serum free of steroid hormones and cholesterol and TG, manufactured by Veritas Corporation)" was used.
[0111] In addition, 12 kinds of surfactants [sodium deoxycholate (manufactured by Fujifilm Wako Pure Chemical Industries, Ltd.), N...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More