Unlock instant, AI-driven research and patent intelligence for your innovation.

Compostion for Treating Wound Comprising Gypenoside LXXV

A technology of gypenoside and composition, which is applied in the direction of medical preparations containing active ingredients, drug combinations, drug delivery, etc., and can solve expensive clinical problems, delayed wound healing, damage, etc.

Inactive Publication Date: 2020-04-28
INTELLIGENT SYNTHETIC BIOLOGY CENT
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Wound healing is delayed or disrupted when any of these phases do not function properly or in a timely manner
This can lead to wounds becoming chronic, which is not only important for the individual, but can be an expensive clinical problem

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Compostion for Treating Wound Comprising Gypenoside LXXV
  • Compostion for Treating Wound Comprising Gypenoside LXXV
  • Compostion for Treating Wound Comprising Gypenoside LXXV

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1: Selection of Ginseng Components for Effective Wound Healing

[0056] Experiments were performed to examine the effects of various ginsenosides on the migration and cell proliferation of HaCaT keratinocytes, which are important processes in wound healing.

Embodiment 1-1

[0057] Example 1-1: Proliferation analysis of HaCaT keratinocytes

[0058] HaCaT keratinocytes were treated with major ginsenosides (e.g., Rb1, Rd, Re, and Rg1) and other ginsenosides (e.g., C-K, F1, F2, G17, G75, PPD, PPT, Rg2, Rg3, Rh1, and Rh2), Concentrations ranged from 0.01 μM to 20 μM for 48 hours before MTT analysis ( figure 1 A). DMSO was used as a control group.

[0059] The results confirmed that various ginsenosides showed effects on cell proliferation to some extent. Specifically, among most ginsenosides, the highest cell proliferation effect was shown when the ginsenoside concentration was 10 μM, while the effect was actually reduced when the ginsenoside concentration was 20 μM.

[0060] In addition, most ginsenosides exhibited about 102% to about 120% cell proliferation effect relative to the control group, while G75 exhibited about 136.8% cell proliferation effect.

Embodiment 1-2

[0061] Example 1-2: Migration analysis of HaCaT keratinocytes

[0062] The wound healing effect of G75 selected in Example 1-1 was examined by scratch wound closure assay.

[0063] Specifically, HaCaT keratinocytes were cultured in a 6-well plate, and the plate was scraped using a 200 μL micropipette tip. The residue was washed with PBS, the cells were cultured for 24 hours, treated with 10 μM ginsenoside or DMSO, and the gap area was measured ( figure 1 B).

[0064] The results confirmed that most ginsenosides showed effects to some extent compared with the control group, and cells treated with G75 showed the highest migration effect.

[0065] In conclusion, G75 showed the highest cell proliferation effect on HaCaT keratinocytes compared with other ginsenosides, and showed the most significant cell proliferation effect at a concentration of 10 μM.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a composition for wound healing or skin regeneration comprising gypenoside 75 (gypenoside LXXV) as an active ingredient.

Description

technical field [0001] The present invention relates to a composition for wound healing or skin regeneration containing Gypenoside LXXV (Gypenoside 75) as an active ingredient. Background technique [0002] The skin is the body's first line of defense against disease and acts as the body's protective barrier, with the epidermis acting as a barrier against invading microorganisms. Therefore, the main goal in treating cuts, burns, abrasions and other injuries of the skin (also known as wounds) is rapid closure and wound healing to prevent infection. [0003] Wound healing clinically means complete closure of the skin, and it is a complex process controlled by the interaction of a variety of cells (e.g., keratinocytes, fibroblasts, endothelial cells, macrophages, platelets, etc.) (Sorg et al., European Surgical Research, 2017; Gonzalez et al., An Bras Dermato, 2016). Each process is controlled by a complex signaling network regulated by many growth factors, cytokines and chem...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/704A61K8/63A61P17/02A61Q19/00
CPCA61K31/704A61K8/63A61P17/02A61Q19/00A61K31/7028A61K8/602A61K9/0014A61K9/12A61K9/06
Inventor 金善昌朴成珠高恩秀
Owner INTELLIGENT SYNTHETIC BIOLOGY CENT