Supercharge Your Innovation With Domain-Expert AI Agents!

Method for preparing rebaudioside M by enzyme technology

A technology of enzymatic preparation and enzymatic activity, applied in the field of rebaudioside M, can solve the problems of limited feasibility, poor economy, high price, etc.

Inactive Publication Date: 2020-04-28
BINZHOU SANYUAN BIOLOGICAL TECH
View PDF6 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the extremely high selling price of UDP-glucose, the feasibility of industrial preparation of Reb M is almost completely limited, and the economy is poor and lacks market competitiveness.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1 Prepare an aqueous phase reaction system, the initial reaction concentration of stevioside is 20 g / L, the initial reaction concentration of ethyl acetate is 100 g / L, the initial reaction concentration of β-cyclodextrin is 100 g / L, palm The initial reaction concentration of acyltransferase was 57U / mL, the initial reaction concentration of cyclodextrin glycosyltransferase was 28U / mL, and the initial reaction concentration of UDP-glycosyltransferase was 114U / mL. The reaction system was adjusted at pH 6 with phosphate buffer. -8.

Embodiment 2

[0015] Example 2 The aqueous phase reaction system in Example 1 was placed in a constant temperature shaker, the reaction temperature was 30° C., and the reaction time was 24 h. An aqueous solution containing rebaudioside M can be prepared, and the concentration of rebaudioside M is determined to be 7.5 g / L by high performance liquid chromatography.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for preparing rebaudioside M by an enzyme technology. The method is mainly characterized in that the rebaudioside M is generated by taking stevioside as a raw material, taking ethyl acetate and beta-cyclodextrin as glycosyl donors and taking palmitoyl transferase, cyclodextrin glycosyl transferase and UDP-glycosyl transferase as main tool enzymes. Expensive UDP-glucose used in the prior art does not need to be adopted as a glycosyl donor; and compared with an existing rebaudioside M preparation method, the method of the invention has great application value andgood economical efficiency.

Description

technical field [0001] The invention uses stevioside as raw material, ethyl acetate and β-cyclodextrin as glycosyl donors, and enzyme components composed of palmitoyltransferase, cyclodextrin glycosyltransferase and UDP-glycosyltransferase as main components. The tool enzyme generates rebaudioside M, and belongs to the technical field of biocatalytic conversion. Background technique [0002] Stevioside (Steviol glycosides) is a natural sweetener extracted from the leaves of Stevia rebaudiana, a herb of Compositae. It is a mixture of various glycosides, and different glycosides have great differences in taste and quality. Stevioside has high sweetness, low calorie, high stability, and potential curative effects such as anti-hyperglycemia, anti-hypertension, anti-inflammation, anti-tumor, anti-diarrhea, and immune regulation effects. It is used as a sweetener in food and Related research and economics in beverages have received extensive attention. High-purity stevia produc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P19/56
CPCC12P19/56
Inventor 谭明宋诙聂在建
Owner BINZHOU SANYUAN BIOLOGICAL TECH
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com