Unlock instant, AI-driven research and patent intelligence for your innovation.

Shell growth medium and noninvasive conchocelis observation method

A technology of shell filaments and filaments, which is applied in botany equipment and methods, horticulture, agriculture, etc., can solve the problems of filaments not being resistant to water loss, unable to polish shells, and dehydration death of filaments, etc., to achieve It is beneficial to the effect of picking seedlings and cultivating

Active Publication Date: 2020-05-01
JIMEI UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The sanding method is obviously time-consuming, labor-intensive, and expensive, and it is impossible to sand the shells to a particularly thin
[0005] The traditional method of observing shell filaments needs to take the shells out of the seawater medium for observation, and the filaments are not resistant to dehydration, and some filaments will die of dehydration after leaving seawater for 30 minutes, so long-term observation is impossible

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: The method of non-injury observation altar seaweed shell filament

[0037] (1) white scallop shell is selected as the raw material of shell growth base;

[0038] (2) Soak the shell growth medium with 0.5mol / L dilute hydrochloric acid for half an hour, and measure the thickness of the shell edge regularly. Take out the shells whose thickness is reduced to less than 0.15mm, rinse with water 3 times and set aside;

[0039] (3) Soak the shell growth substrate treated with dilute acid in seawater, and boil and sterilize for 10 minutes;

[0040] (4) Spread the sterilized shell growth medium on the bottom of the container, add PES medium, then crush the free filaments of Porphyra alba, add them to the container, stir gently to promote the uniform dispersion of the free filaments.

[0041] (5) Take a small piece of shell regularly, place it in an observation device equipped with seawater culture medium, observe the transparent area of ​​the shell with a microscope...

Embodiment 2

[0043] Embodiment 2: The method of non-injury observation Porphyra zebra shell filaments

[0044] (1) The raw material of the shell growth base is the thin slices (thickness is about 0.5 mm) that are cut from mussel shells;

[0045] (2) Soak the shell growth medium with 0.25mol / L dilute sulfuric acid for half an hour, and measure the shell edge thickness regularly. Take out the shells whose thickness is reduced to less than 0.15mm, rinse with water 3 times and set aside;

[0046] (3) Soak the shell growth base treated with dilute acid in seawater, boil and sterilize for 15 minutes;

[0047] (4) Spread the sterilized shell growth medium on the bottom of the container, add PES medium, then add the Porphyra zebra fruit spore water into the container, stir gently to promote uniform dispersion, and make the seedlings more uniform.

[0048] (5) Take a small piece of shell, scrub the surface with sterile gauze, and then place it in an observation device equipped with seawater cultu...

Embodiment 3

[0049] Embodiment 3: the method for observing red cabbage shell filaments without damage

[0050] (1) white scallop shell is selected as the raw material of shell growth base;

[0051] (2) Soak the shell growth medium with 0.5mol / L dilute hydrochloric acid for half an hour, and measure the thickness of the shell edge regularly. Take out the shells whose thickness is reduced to less than 0.15mm, rinse with water 3 times and set aside;

[0052] (3) Soak the shell growth substrate treated with dilute acid in seawater, and boil and sterilize for 10 minutes;

[0053] (4) Spread the sterilized shell growth base on the bottom of the container, add PES medium, then add the red cabbage fruit spores or crushed free filaments into the container, and gently stir to promote uniform dispersion.

[0054] (5) Take a small piece of shell, scrub the surface with sterile gauze, and then place it in an observation device equipped with seawater culture medium. The transparent area of ​​the shell...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Thicknessaaaaaaaaaa
Thicknessaaaaaaaaaa
Thicknessaaaaaaaaaa
Login to View More

Abstract

The invention relates to a shell growth medium and a noninvasive conchocelis observation method. The shell growth medium is prepared by treating white shells by dilute acid until the thickness does not exceed 0.15 mm, and then conchocelis grows in the shells. Further, according to the noninvasive conchocelis observation method, the shell conchocelis is put into a culture solution, the growth and development of the porphyra shell conchocelis are observed through a microscope; the treatment of the shell conchocelis by a shell dissolving agent with a damage effect is not needed; and the growth condition of the conchocelis can be continuously observed for a long time. By using the method provided by the invention, a large number of transparent shell growth media can be simply, conveniently andfast prepared; the noninvasive in-situ observation of the porphyra shell conchocelis can be realized; the growth and development condition of the porphyra shell conchocelis can be clearly and continuously observed and recorded; and the foundation is laid for developing conchocelis-related production technologies. The method can also be used for algae, such as bangia atropurpurea, with life histories including a shell conchocelis stage.

Description

technical field [0001] The invention relates to the technical field of filamentous seedling cultivation, in particular to a shell growth substrate and a method for non-damaging filamentous observation. Background technique [0002] The life cycle of economic seaweeds such as Porphyra and Rubica sativa includes generations of filaments, such as Porphyra filaments and Rubiza filaments. Taking Porphyra filaments as an example, it belongs to the micro-generations in the life history of Porphyra. The life history of Porphyra is divided into the diploid filament stage and the haploid thallus stage. Porphyra filaments can dissolve calcium, drill into calcium-rich shells, and form shell filaments to survive the high temperature in summer. The shell filaments mature in autumn and then release conchospores. At each stage of the maturation process, there are special needs for light, mild nutrients and salts. In recent years, there has been a demand for "nurture in the south and rear ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01G33/02
CPCA01G33/00
Inventor 许凯谢潮添纪德华徐燕王文磊陈昌生
Owner JIMEI UNIV