Preparation method of algistat containing gingko endophyte

Abstract

The invention discloses a preparation method of an algistat containing gingko endophyte, and belongs to the technical field of water treatment. Ginkgo biloba endophyte extracted from ginkgo bark and leaves is attached to algistat components; the ginkgo biloba endophyte has good sterilization and bacteriostasis capability; the pollution of the algistat to the environment can also be reduced by utilizing the gingko endophyte; meanwhile, plant fibers, lignin and other components are extracted from the ginkgo barks and leaves; carbonized fibers are generated in the subsequent carbonization process; meanwhile, carbonized micro-particles formed by carbonizing sugar molecules are embedded into the carbon nano-particles; the algae removal efficiency of the algistat is enhanced; the effect is quick; a silicon dioxide component is also introduced into the fiber component; silicon dioxidete components are adsorbed in the plant fibers, silicon dioxide nano-molecules are generated in a carbon fiberstructure again after the pH value is adjusted and high-temperature treatment is conducted, the surface roughness of carbon fibers is improved, meanwhile, the carbon fibers are coated and protected to a certain extent, the growth environment of the gingko endophytes is protected, and the algae removal time efficiency of the algistat is improved.

Description

technical field

[0001] The invention discloses a preparation method of an algastatic agent containing endophytic bacteria of ginkgo, belonging to the technical field of water body treatment. Background technique

[0002] With the development of economy and the increase of human activities, the eutrophication of lakes and reservoirs is becoming more and more serious. The algae bloom caused by eutrophication not only brings serious harm to the ecosystem, but also seriously threatens human health and survival, increases the cost of water treatment and maintenance, makes the water ecological environment unbalanced, and becomes a water resource environment. One of the major challenges facing the field.

[0003] Algae bloom is a disaster phenomenon that frequently occurs in seawater and freshwater waters. It is called red tide in the ocean and algae bloom in freshwater. The algae that can form water blooms are mainly species in Cyanophyta and Chlorophyta. In eutrophic freshwate...

Examples

Embodiment 1

[0031] Preparation of heat treatment reaction solution:

[0032] Ginkgo bark and ginkgo leaves are put into pulverizer according to the mass ratio of 5:1 to make a mixture, put the mixture into an oven, and dry for 2 hours at a temperature of 40°C to obtain a dry material, and mix the dry material with Put absolute ethanol into a beaker at a mass ratio of 1:10, place the beaker in a shaker, oscillate and soak for 3 hours, and after soaking, filter with suction to separate the filter residue and filtrate, and set aside;

[0033] Put the above filter residue into the reaction kettle, soak it with hydrochloric acid with a mass fraction of 3%, seal the reaction kettle, raise the temperature inside the reaction kettle to 60°C, and stir with a stirrer at a speed of 400r / min for 2 hours under constant temperature conditions. After stirring, Dropwise add a mass fraction of 10% sodium hydroxide solution to the beaker to adjust the pH value to 10 to prepare an alkaline mixed solution; ...

Embodiment 2

[0041] Preparation of heat treatment reaction solution:

[0042] Ginkgo bark and ginkgo leaves are put into the pulverizer according to the mass ratio of 5:1 and pulverized to obtain a mixture, the mixture is put into an oven, and dried for 2.5 hours at a temperature of 45°C to obtain a dry material, and the dried material Put it into a beaker with anhydrous ethanol at a mass ratio of 1:10, place the beaker in a shaker, oscillate and soak for 3.5 hours, after soaking, filter and separate by suction to obtain the filter residue and filtrate, and set aside;

[0043] Put the above filter residue into the reaction kettle, soak it with hydrochloric acid with a mass fraction of 5%, seal the reaction kettle, raise the temperature in the reaction kettle to 65°C, stir with a stirrer at a speed of 450r / min for 2.5h under constant temperature conditions, and stir Afterwards, dropwise add a mass fraction of 12% sodium hydroxide solution to the beaker to adjust the pH value to 11 to prepar...

Embodiment 3

[0051] Preparation of heat treatment reaction solution:

[0052] Put the ginkgo bark and ginkgo leaves into the pulverizer at a mass ratio of 5:1 to make a mixture, put the mixture into an oven, and dry it for 3 hours at a temperature of 50°C to obtain a dry material, and mix the dry material with Put absolute ethanol into a beaker at a mass ratio of 1:10, place the beaker in a shaker, oscillate and soak for 4 hours, and after soaking, filter with suction to separate the filter residue and filtrate for later use;

[0053] Put the above filter residue into the reaction kettle, soak it with hydrochloric acid with a mass fraction of 6%, seal the reaction kettle, raise the temperature in the reaction kettle to 70°C, and stir with a stirrer at a speed of 500r / min for 3h under constant temperature conditions. After stirring, Dropwise add a mass fraction of 15% sodium hydroxide solution to the beaker to adjust the pH value to 12 to prepare an alkaline mixed solution;

[0054] Put th...

technical field

[0001] The invention discloses a preparation method of an algastatic agent containing endophytic bacteria of ginkgo, belonging to the technical field of water body treatment. Background technique

[0002] With the development of economy and the increase of human activities, the eutrophication of lakes and reservoirs is becoming more and more serious. The algae bloom caused by eutrophication not only brings serious harm to the ecosystem, but also seriously threatens human health and survival, increases the cost of water treatment and maintenance, makes the water ecological environment unbalanced, and becomes a water resource environment. One of the major challenges facing the field.

[0003] Algae bloom is a disaster phenomenon that frequently occurs in seawater and freshwater waters. It is called red tide in the ocean and algae bloom in freshwater. The algae that can form water blooms are mainly species in Cyanophyta and Chlorophyta. In eutrophic freshwate...

Examples

Embodiment 1

[0031] Preparation of heat treatment reaction solution:

[0032] Ginkgo bark and ginkgo leaves are put into pulverizer according to the mass ratio of 5:1 to make a mixture, put the mixture into an oven, and dry for 2 hours at a temperature of 40°C to obtain a dry material, and mix the dry material with Put absolute ethanol into a beaker at a mass ratio of 1:10, place the beaker in a shaker, oscillate and soak for 3 hours, and after soaking, filter with suction to separate the filter residue and filtrate, and set aside;

[0033] Put the above filter residue into the reaction kettle, soak it with hydrochloric acid with a mass fraction of 3%, seal the reaction kettle, raise the temperature inside the reaction kettle to 60°C, and stir with a stirrer at a speed of 400r / min for 2 hours under constant temperature conditions. After stirring, Dropwise add a mass fraction of 10% sodium hydroxide solution to the beaker to adjust the pH value to 10 to prepare an alkaline mixed solution; ...

Embodiment 2

[0041] Preparation of heat treatment reaction solution:

[0042] Ginkgo bark and ginkgo leaves are put into the pulverizer according to the mass ratio of 5:1 and pulverized to obtain a mixture, the mixture is put into an oven, and dried for 2.5 hours at a temperature of 45°C to obtain a dry material, and the dried material Put it into a beaker with anhydrous ethanol at a mass ratio of 1:10, place the beaker in a shaker, oscillate and soak for 3.5 hours, after soaking, filter and separate by suction to obtain the filter residue and filtrate, and set aside;

[0043] Put the above filter residue into the reaction kettle, soak it with hydrochloric acid with a mass fraction of 5%, seal the reaction kettle, raise the temperature in the reaction kettle to 65°C, stir with a stirrer at a speed of 450r / min for 2.5h under constant temperature conditions, and stir Afterwards, dropwise add a mass fraction of 12% sodium hydroxide solution to the beaker to adjust the pH value to 11 to prepar...

Embodiment 3

[0051] Preparation of heat treatment reaction solution:

[0052] Put the ginkgo bark and ginkgo leaves into the pulverizer at a mass ratio of 5:1 to make a mixture, put the mixture into an oven, and dry it for 3 hours at a temperature of 50°C to obtain a dry material, and mix the dry material with Put absolute ethanol into a beaker at a mass ratio of 1:10, place the beaker in a shaker, oscillate and soak for 4 hours, and after soaking, filter with suction to separate the filter residue and filtrate for later use;

[0053] Put the above filter residue into the reaction kettle, soak it with hydrochloric acid with a mass fraction of 6%, seal the reaction kettle, raise the temperature in the reaction kettle to 70°C, and stir with a stirrer at a speed of 500r / min for 3h under constant temperature conditions. After stirring, Dropwise add a mass fraction of 15% sodium hydroxide solution to the beaker to adjust the pH value to 12 to prepare an alkaline mixed solution;

[0054] Put th...