The invention relates to a preparation process of a moisture absorption and sweat releasing fabric, and belongs to the technical field of fabric preparation processes. The preparation process specifically comprises the following process steps: S1, spinning, namely, spinning a fiber A into a first yarn, and spinning a fiber B into a second yarn; S2, weaving, namely, by taking the first yarn obtained in the step S1 as a raw material of inner-layer cloth and the second yarn obtained in the step S1 as a raw material of outer-layer cloth, carrying out weaving to obtain double-layer gray fabric; andS3, dyeing and finishing, specifically comprising alkali deweighting, dyeing, soaping and shaping, so that the finished product fabric is obtained, wherein the fiber A is a hydrophilic fiber, and thefiber B is a hydrophobic fiber. The moisture absorption and sweat releasing fabric has the effect of being higher in moisture absorption and sweat releasing capacity.