Method for evaluating ecological risk safety threshold of toxic microalgae
An ecological risk and safety threshold technology, applied in the field of ecological risk assessment of red tide microalgae, can solve the problems of inability to use species sensitivity distribution, low data integrity and universality, and large algae biological variability, and achieve a wide range of applications , accurate evaluation and simple operation
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Embodiment 1
[0023] Ecological risk safety threshold assessment of Protocercia reticulum:
[0024] The specific evaluation steps are:
[0025] Isolation and cultivation of S1 algae: Single microalgae cells are picked from the natural seawater that has exploded and multiplied. Protozoa reticulata are collected from the Changhai sea area of Dalian, and single-celled algae strains are picked by capillary tubes for purification and culture. First use 96-well plate and original seawater (0.45μm membrane filter) for pure culture, then gradually change to 24-well plate and finally transfer to Erlenmeyer flask, and use desiliconized f / 2 culture medium for culture. Other conditions are temperature 15°C, light intensity 4000lux, light-to-dark ratio 12h:12h. An appropriate amount of culture solution was added every other week, and the growth status was observed with an inverted microscope (Olympus IX71, Japan) every day during the culture period.
[0026] Acquisition of S2 toxicity data: Protocyt...
Embodiment 2
[0039] Ecological Risk Safety Threshold Assessment of Alexandrium minutis:
[0040] The specific evaluation steps are:
[0041] Isolation and cultivation of S1 algae: the pure species of Alexandrium minutiae used in this research is purchased and cultivated in our laboratory. After inoculation with sterilized seawater, Alexandrium minutisii was inoculated at 20°C, 4000 lx, and the cycle was 12h:12h Expanded culture in a light incubator.
[0042] Acquisition of S2 toxicity data: the experimental organisms (brine shrimp, seawater medaka, golden algae, chlorella, sclerophyllum, and Thalassiosira pseudodwarf) were obtained as above. Algal cell counts were the same as above. The paralytic microalgae toxins contained in Alexandrium microalgae were extracted and analyzed according to standard specifications. The toxicity test method is the same as above. Calculate the toxicity value LC50 or EC50.
[0043] Construction of the S3 model: the paralytic shellfish toxin obtained from ...
Embodiment 3
[0047] Ecological risk safety threshold assessment of Prorocentrum lima:
[0048] The specific evaluation steps are:
[0049] Isolation and cultivation of S1 algae: The pure species of Prorodinium lima used in this study is purchased, and has been kept in our laboratory for cultivation. After inoculation with sterilized seawater, Prorodinova lima was grown under the conditions of 20°C, 4000 lx, Cycle 12h: expand the culture in a 12h light incubator.
[0050] Acquisition of S2 toxicity data: the experimental organisms (brine shrimp, seawater medaka, golden algae, chlorella, sclerophyllum, and Thalassiosira pseudodwarf) were obtained as above. Algal cell counts were the same as above. The diarrheal shellfish toxin contained in Prorodinium lima was extracted and analyzed according to standard specifications. The toxicity test method is the same as above. Calculate the toxicity value LC50 or EC50.
[0051] Construction of the S3 model: the diarrheal shellfish toxin (converted...
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